Effect of long‐distance transportation on serum metabolic profiles of steer calves

Takemoto, Satoshi; Tomonaga, Shozo; Funaba, Masayuki; Matsui, Tohru

doi:10.1111/asj.12870

Cited by 15 publications

(13 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Serum metabolomics analysis was performed using GC/MS as described previously ( Takemoto et al., 2017 ) with some modifications. A sample of 50 μL of serum was mixed with 5 μL of 1 mg/mL 2-isopropylmalic acid (Sigma-Aldrich, Tokyo, Japan) in distilled water as an internal standard, and 250 μL of a methanol–chloroform–water (2.5:1:1) mixture.…”

Section: Methodsmentioning

confidence: 99%

The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature

Usuda

Nagaoka

et al. 2020

Poultry Science

View full text Add to dashboard Cite

Reproductive failure associated with heat stress is a well-known phenomenon in poultry. High temperatures also induce various metabolic disturbances in many animals. Because the liver plays a central role in metabolism, the present study aimed to clarify the relationship between liver and reproduction in Japanese quails exposed to high temperatures. In the consecutive 20-D experimental period, quails were treated with 25°C (control) or 34°C (heat) from 12:00 to 16:00. Eggs were collected for hatching. On completion of the experimental period, quails were humanely euthanized for hormone analyses (e.g., serum and ovarian follicles). Serum metabolites were analyzed using GC/MS. Liver and ovary samples were collected for mRNA levels, histomorphology, and metabolic analysis. Ovary and oviduct weights significantly decreased after daily heat exposure. The number and weight of hierarchical follicles also decreased. Consequently, egg weight decreased. Although there was no difference in fertilization rate, chick birth weight significantly decreased in the heated group. Corticosterone and 17β-estradiol in the serum significantly increased in the heated group. Yolk corticosterone and 17β-estradiol concentration and content were higher in the heated group. Ovary sterologenic enzymes gene P450scc and estrogen receptor expression level increased. The FSH receptor decreased in heat-stressed quails. MetaboAnalyst analysis indicated that high temperature affects propanoate metabolism, beta-alanine metabolism, aspartate metabolism, and histidine metabolism. Triglyceride and cholesterol levels in the liver increased in the heated group. The heated group also had an increased mRNA expression of AGPAT5 , apoptosis gene caspase3 , and the immunocytokine genes IL-6 and TLR4 . However, NF-κB gene expression decreased. These results suggest that high temperatures affect lipid metabolism and apoptosis and cause inflammation in the liver. High temperature induced ovarian dysfunction, which resulted in the decline of hierarchical follicle number and weight, egg weight, and chick birth weight. The increased level of 17β-estradiol suggests liver damage. Protecting liver function from damage may assist quails cope in summer.

show abstract

Section: Methodsmentioning

confidence: 99%

The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature

Usuda

Nagaoka

et al. 2020

Poultry Science

View full text Add to dashboard Cite

show abstract

“…On the other hand, niacin supplementation did not affect niacin concentration in blood immediately after transportation; blood niacin concentration started to increase 3 days after the initiation of its supplementation. Although we reported that serum nicotinamide concentration decreased for 3 days after transportation with feed and water deprivation in steer calves (Takemoto et al., ), the reduction in blood niacin concentration was not observed in the TRANS group. Niacin in erythrocyte is its major form in blood (McDowell, ).…”

Section: Discussionmentioning

confidence: 59%

“…Transportation with feed and water deprivation continuously decreased serum GLU for 30 days after the transportation but serum GLU concentration temporarily decreased in the REST group just after the period of feed and water deprivation. Our previous experiment also indicated that transportation with deprivation of feed and water decreased serum GLU concentration for 7 days in steer calves (Takemoto et al., ). Ishiwata et al.…”

Section: Discussionmentioning

confidence: 70%

“…Because niacin in feedstuff has poor stability in the rumen, the nutritional status of niacin does not depend on its intake, but on its synthesis in the rumen and the liver of cattle (Hungate, ; Scott, Nesheim, & Young, ). Previously, we found that the transportation with feed and water deprivation decreased serum nicotinamide concentration in steer calves for 3 days after transportation (Takemoto, Tomonaga, Funaba, & Matsui, ). Therefore, transportation with feed and water deprivation was considered to induce the shortage of niacin.…”

Section: Introductionmentioning

confidence: 88%

See 1 more Smart Citation

Effect of niacin supplementation in long‐distance transported steer calves

Takemoto

Funaba

Matsui

2018

Animal Science Journal

Self Cite

View full text Add to dashboard Cite

Long-distance transportation has negative impacts on production and health in cattle. Feed and water are routinely deprived from cattle during transportation. We investigated whether niacin supplementation could improve niacin nutrition and mitigate the adverse effect of transportation with feed and water deprivation in steer calves. We also studied the adverse effect of feed and water deprivation in nontransported steer calves. Twelve calves were assigned to feed and water deprivation for 2 days, or full access to feed and water in experiment 1. Ten calves were assigned to 2-day transportation with feed and water deprivation, or the transportation with feed and water deprivation, but with supplementation of rumen-protected niacin at 100 g/day per head in experiment 2. Bodyweight was measured and blood was collected for 32 days in each experiment. Feed and water deprivation temporarily decreased serum glucose concentrations and bodyweight gain. Transportation with deprivation of feed and water caused a temporal decrease in bodyweight gain and serum albumin concentration, and a continuous decrease in serum glucose and total cholesterol concentrations, which was suppressed by niacin supplementation. Niacin supplementation increased blood niacin concentration. These results suggest that niacin supplementation mitigates adverse effects of transportation with feed and water deprivation in steer calves.

show abstract

“…A serum metabolomics analysis was performed using GC/MS as described previously [24] with some modifications. In brief, a sample of 50 µl of serum was mixed with 5 µl of 1 mg/m l 2-isopropylmalic acid (Sigma-Aldrich, Tokyo, Japan) in distilled water as an internal standard, and 250 µl of methanol–chloroform–water (2.5:1:1) mixture.…”

Section: Methodsmentioning

confidence: 99%

Heat challenge influences serum metabolites concentrations and liver lipid metabolism in Japanese quail (Coturnix japonica)

Usuda

Nagaoka

et al. 2019

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

High temperature induces various metabolic disturbances in animals. However, no comprehensive information is currently available on the metabolic pathway affected by high environmental temperature. The present study examined metabolite content in the serum of heat challenged quails using metabolomic analysis. In the present study, female quails with normal laying rate at 20 weeks kept in standard condition (control group) or exposed to 34°C 4 hr per day (12:00 to 16:00 hr)(heat group) for 10 consecutive days. The metabolomic analysis identified 165 metabolites in the serum, and significant differences were observed in the serum for 7 metabolites between two groups. An analysis by MetaboAnalyst, a web-based metabolome data tool, indicate that high temperature affect ketone body metabolism, butyrate metabolism, arginine and proline metabolism. Furthermore, histological examination of liver indicates a heat challenge induced abnormal lipid metabolism. Triglyceride and cholesterol level in the liver increased, however cholesterol level decreased in the serum. Genes related to lipid metabolism significantly increased in the liver after heat challenge. The present study demonstrated that high temperature cause liver damage, thus lipid metabolic was affected. Protect liver under high temperature could be one solution for coping with high temperatures in summer.

show abstract

Effect of long‐distance transportation on serum metabolic profiles of steer calves

Cited by 15 publications

References 39 publications

The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature

The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature

Effect of niacin supplementation in long‐distance transported steer calves

Heat challenge influences serum metabolites concentrations and liver lipid metabolism in Japanese quail (Coturnix japonica)

Contact Info

Product

Resources

About