Effect of locally injected autologous platelet-rich plasma on second intention wound healing of acute full-thickness skin defects in dogs

Karayannopoulou, Maria; Psalla, Dimitra; Kazakos, George; Loukopoulos, Panayiotis; Giannakas, N.; Savvas, Ioannis; Kritsepi‐Konstantinou, Maria; Chantes, Achilleas; Papazoglou, Lyssimachos

doi:10.3415/vcot-14-06-0088

Cited by 35 publications

(23 citation statements)

References 36 publications

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“…In previous study, Alsabaawe, Hom et al, have been suggested an increased of epithelialization rate and regression of acute inflammatory process in PRP treated skin wounds in rabbits during week 1 postoperative period compared to week 2 where there was no statistical differences found in epithelial thickness and inflammation stage between PRP treated wound and control. Also, they indicated that the epithelialization returned to normal texture and improvement of wound healing in term of reducing the inflammatory process [ 35 , 36 ]. On the other hand, Ahmad et al, (2012) suggested that the PRP has the potential to enhance the healing of tissue at the cellular level via the recruitment proliferation, and differentiation of cells involved in tissue regeneration [ 37 ] Moreover, Gotterbarm et al, (2006) have been reported the PRP acts as a rich source of autologous growth factors [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Effect of platelet-rich plasma on the healing of cutaneous defects exposed to acute to chronic wounds: a clinico-histopathologic study in rabbits

et al. 2015

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BackgroundPlatelet-rich plasma (PRP) contains numerous growth factors to promote wound healing and angiogenesis. The aim of this study was to gain further information about the benefits of platelet-rich-plasma for healing cutaneous acute to chronic wounds.MethodsA total of 30 New Zealand albino rabbits (n = 15/group) were randomly assigned to two experimental groups: control group, and PRP group. Bilateral resection defects measuring 3 cm were surgically created on the dorsolateral of the cutaneous in animals and the defects were randomly divided into two mentioned groups. Wound area, neovascularization, size and epithelialization were compared on days 7, 14 and 21 post-wounding. Histopathological analyses were conducted on 15 specimens from each group after sacrifice by the cellular aspects of the regeneration of the tissue.ResultsOur results were indicated that the wound area of PRP was smaller than that in the non-treated group on days 7, 14 and 21. Furthermore, a significant decrease of the wound size was observed in PRP groups that were significantly greater than that in the control group. A significant increase of the mean vascular density was noted in the PRP treated groups compared to the control groups at day 14 and especially day 21. This results indicated that PRP treated group’ enhanced angiogenesis at the wound beds as compared to no treatment group.ConclusionThese results could be useful for researchers in the growing fields of tissue repair and experimental wound healing. Further studies will be essential to determine the role of PRP in clinical practice.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Effect of platelet-rich plasma on the healing of cutaneous defects exposed to acute to chronic wounds: a clinico-histopathologic study in rabbits

et al. 2015

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“…Although the forest plot in Fig 3 showed two studies with not significant negative ES [ 51 , 53 ], two studies with null ES [ 30 , 46 ], seven studies with not significant positive ES [ 42 , 44 , 45 , 50 , 52 , 56 , 58 ] and four studies with significant positive ES [ 43 , 54 , 55 , 57 ]: the exclusion of each of the studies, in the sensitivity analysis, would have no relevant effect on the overall results of the meta-analysis supporting the efficacy of PRP for primary outcome. ( Fig 4 )…”

Section: Resultsmentioning

confidence: 99%

“…This finding is associated with a moderate degree of heterogeneity. The quality of evidence obtained in the meta-analysis for the primary outcome was demonstrated by sensitivity analysis, excluding alternately each of the studies, even showing negative ES [ 51 , 53 ], null ES [ 30 , 46 ], or statistically positive ES [ 43 , 54 , 55 , 57 ]: no relevant changes occurred in the results of the meta-analysis of primary outcome, persisting in statistical agreement with the hypothesis that supports the superiority of the PRP group. The sensitivity analysis finds its own indication in the identification of potential "outlier studies".…”

Section: Discussionmentioning

confidence: 99%

Platelet-rich plasma to treat experimentally-induced skin wounds in animals: A systematic review and meta-analysis

et al. 2018

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The objective of the study was to review current literature to determine whether the topical application of platelet-rich plasma (PRP) promotes healing in experimentally-induced full-thickness skin wounds in animals. The hypothesis was that the adjunct of PRP has a positive effect on wound healing. An electronic search was carried out on the following databases: Web of Science, Cochrane Library, PubMed, Research Gate, Cochrane Wounds Group, Veterinary Information Network. No publication date nor language restrictions were applied. Randomised and not randomised controlled clinical trials comparing PRP with placebo or with other treatments were included. The reduction of open wound area in PRP-treated (test) wounds compared to control wounds was the primary outcome. Secondary outcomes were healing time and number of healed cases in test group compared to control. The following effect sizes were calculated: the Hedges’ g for continuous variables; the odds ratio for binary data. Eighteen controlled clinical trials were included in the qualitative and quantitative synthesis, with a total of 661 wounds. All studies were published in the period 2007–2016. Eight studies were carried out on rodent/lagomorph mammals and 10 on non-rodent/lagomorph mammals. In all included studies, control wounds underwent placebo or were left untreated. The PRP group showed a better healing performance than the control group in each outcome. The effect size was statistically significant considering the primary outcome and the overall aggregation of the three outcomes. The effect size, although in favour of the treatment with PRP, was not significant considering the healing time and the number of healings. The overall heterogeneity was mild or moderate. Five studies reported a high risk of selection bias. The publication bias was always mild or absent. The results support the hypothesis of the positive effects of the PRP when compared to control groups in the treatment of experimentally-induced full-thickness skin wounds in animals. PRP can therefore be considered an effective adjunctive therapy in stimulating second intention healing of acute wounds in healthy animals.

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“…Finally, we designed our study as a pilot study of a clinical innovative therapy on a small number of subjects following the literature on some similar studies on new treatments in dermatology [ 26 , 32 , 33 ]. We preferred to use the “case-control” study design on the same subject to reduce the variables of clinical response and avoid confounding factors such as age, race, and gender.…”

Section: Discussionmentioning

confidence: 99%

Low-level laser therapy: Case-control study in dogs with sterile pyogranulomatous pododermatitis

Perego¹,

Proverbio²,

Zuccaro³

et al. 2016

Vet World

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Aim:Low-level laser therapy (LLLT) is a therapeutic photobiostimulation with properties in reducing swelling, inflammation, and promoting tissue healing. The objective of this pilot study was to evaluate LLLT in sterile pyogranulomatous pododermatitis in five dogs.Materials and Methods:In each dog, one lesion was designated as the control (treated with a 0.0584% hydrocortisone aceponate spray), and one or more other lesions were treated with a gallium aluminum arsenide-laser, daily for 5 days. Lesions were scored before treatment (D0), at the end (D4), 16 days after the last laser treatment (D20), and after 2 months (D65).Results:Comparing the treated lesion group with the control lesion group, the clinical score was similar at D0, whereas there was a statistically significant difference at D4 and D20; in the treated group over time, there was a statistically significant improvement between D0, D4, and D20. Lesion recurrence was absent in more than 50% of the treated lesions at D65. No adverse reactions were reported.Conclusion:Given the positive results of this first clinical study, it would be interesting to extend the study to confirm the validity of this type of therapy in sterile pyogranulomatous pododermatitis in the dog.

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Effect of locally injected autologous platelet-rich plasma on second intention wound healing of acute full-thickness skin defects in dogs

Abstract: Locally injected autologous PRP does not accelerate the healing process, but increases tissue perfusion and may promote the formation of organized collagen bundles in acute full-thickness skin defects in dogs.

Cited by 35 publications

References 36 publications

RETRACTED ARTICLE: Effect of platelet-rich plasma on the healing of cutaneous defects exposed to acute to chronic wounds: a clinico-histopathologic study in rabbits

RETRACTED ARTICLE: Effect of platelet-rich plasma on the healing of cutaneous defects exposed to acute to chronic wounds: a clinico-histopathologic study in rabbits

Platelet-rich plasma to treat experimentally-induced skin wounds in animals: A systematic review and meta-analysis

Low-level laser therapy: Case-control study in dogs with sterile pyogranulomatous pododermatitis

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