Effect of liver fatty acid binding protein (L-FABP) gene ablation on lipid metabolism in high glucose diet (HGD) pair-fed mice

Abstract

Liver fatty acid binding protein (L-FABP) is the major fatty acid binding/"chaperone" protein in hepatic cytosol. Although fatty acids can be derived from the breakdown of dietary fat and glucose, relatively little is known regarding the impact of L-FABP on phenotype in the context of high dietary glucose. Potential impact was examined in wild-type (WT) and Lfabp gene ablated (LKO) female mice fed either a control or pair-fed high glucose diet (HGD). WT mice fed HGD alone exhibited decreased whole body weight gain and weight gain/kcal food consumed-both as reduced lean tissue mass (LTM) and fat tissue mass (FTM). Conversely, LKO alone increased weight gain, lean tissue mass, and fat tissue mass while decreasing serum β-hydroxybutyrate (indicative of hepatic fatty acid oxidation)-regardless of diet. Both LKO alone and HGD alone significantly altered the serum lipoprotein profile and increased triacylglycerol (TG), but in HGD mice the LKO did not further exacerbate serum TG content. HGD had little effect on hepatic lipid composition in WT mice, but prevented the LKO-induced selective increase in hepatic phospholipid, free-cholesterol and cholesteryl-ester. Taken together, these findings suggest that high glucose diet diminished the effects of LKO on the whole body and lipid phenotype of these mice.

Effects of dietary supplementation of astaxanthin (Ast) on growth performance, activities of digestive enzymes, antioxidant capacity and lipid metabolism of large yellow croaker ( Larimichthys crocea ) larvae

“…L‐FABP, apoB and mtp are gens related to the transportation of lipid. L‐FABP is the protein that major in promoting uptake and transport in liver cytoplasm (McIntosh et al, 2019). The mtp gene is responsible for packaging TG in the transportation of lipid (Schlegel & Stainier, 2006), while apoB was proved to essential for the hepatic assemble and secretion of TG (Storch & Thumser, 2010; Yan et al, 2017).…”

Upregulation of Hepatic Glutathione S-Transferase Alpha 1 Ameliorates Metabolic Dysfunction-Associated Steatosis by Degrading Fatty Acid Binding Protein 1

“…It is mainly responsible for lipid metabolism, including fatty acid uptake, trafficking, and lipid storage [48]. Studies have previously shown that FABP1 mainly transports fatty acids to the liver and converts them into TG and phospholipids, thereby participating in intracellular fatty acid homeostasis [49,50], or directly interacts with fatty acyl-CoA synthases to donate bound long-chain fatty acids (LCFAs) for conversion to LCFA-CoAs, thereby enhancing the uptake of LCFA and cholesterol in living cells and promoting fat storage in the liver [49,51,52]. Therefore, FABP1 plays a critical role in regulating the metabolism underlying hepatic steatosis.…”

Effect of dietary supplementation with sanguinarine on meat quality and lipid metabolism of broilers

“…According to previous studies, the enhancement of the activity of PPARα and PPARγ in tissues can regulate fat metabolism and reduce the accumulation of fat in tissues ( Lee et al, 2012 ; Gu et al, 2015 ). Extensive research indicates that by increasing the expression of downstream target genes in the PPAR pathway, lipid metabolism processes can be regulated, such as converting cholesterol metabolism to synthesize bile acids, fatty acid transport to regulate fat deposition, and fatty acid oxidation decomposition to produce energy ( Guo et al, 2018 ; McIntosh et al, 2019 ; Anagnostopoulos et al, 2022 ; Zhao et al, 2022 ). By molecular docking results, we found that SAN could interact with PPARγ, PPARα and FABP1 , suggesting that SAN can regulate PPAR -mediated lipid metabolism signaling pathway.…”