Effect of liquid nitrogen vapor storage on the motility, viability, morphology, deoxyribonucleic acid integrity, and mitochondrial potential of frozen-thawed human spermatozoa

Lim, Jinho; Shin, Tai Eun; Song, Seung-Hun; Bak, Chong Won; Yoon, Tae Ki; Lee, Dong Ryul

doi:10.1016/j.fertnstert.2010.02.051

Cited by 23 publications

(18 citation statements)

References 17 publications

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“…Sperm samples can be stored in the vapor for up to three months without inducing significant effects on sperm motility, viability, and morphology. DNA integrity and mitochondrial potential are similar for both vapor and LN 2 storage methods [Lim et al 2010].…”

Section: Liquid Nitrogen Vapor Freezingmentioning

confidence: 86%

“…C [Lim et al 2010]. The optimal height for storage was found by storing the sample at different heights for a period of one week.…”

Section: Liquid Nitrogen Vapor Freezingmentioning

confidence: 99%

“…Table 3 illustrates the advantages and disadvantages of the main storage methods compared to conventional cryopreservation. [Lim et al 2010] Storage using empty zona For storing small number of spermatozoa 1. Acceptable sperm recovery, survival, and fertilization rate 1.…”

Section: Future Of Cryopreservationmentioning

confidence: 99%

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Effect of sperm storage and selection techniques on sperm parameters

Sharma

Kattoor

Ghulmiyyah

et al. 2014

Systems Biology in Reproductive Medicine

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Sperm cryopreservation preserves the fertility of cancer patients undergoing chemotherapy, ensures sperm are available at the time of oocyte retrieval in assisted reproductive technology (ART) procedures and avoids the need for repeated sperm extraction surgeries in azoospermic patients. Conventional methods of cryopreservation involve storage in liquid nitrogen (LN 2 ), which causes a significant decline in sperm parameters such as motility and viability and results in DNA damage. Newer methods of sperm cryopreservation such as the LN 2 vapor method, vitrification, and experimental methods such as lyophilization, have significant advantages over the conventional methods in terms of cost effectiveness and ease of use. Density gradient centrifugation (DGC), swim up, and magnetic assisted cell sorting (MACS) can be used prior to or post-cryopreservation to improve post-thaw sperm quality. Cryopreservation in special carriers such as cryoloops and empty zona prevents the loss of small numbers of sperm during cryopreservation. This article will discuss these sperm preservation and selection techniques.

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Section: Liquid Nitrogen Vapor Freezingmentioning

confidence: 86%

“…C [Lim et al 2010]. The optimal height for storage was found by storing the sample at different heights for a period of one week.…”

Section: Liquid Nitrogen Vapor Freezingmentioning

confidence: 99%

See 1 more Smart Citation

Effect of sperm storage and selection techniques on sperm parameters

Sharma

Kattoor

Ghulmiyyah

et al. 2014

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

show abstract

“…But their temperatures were lower than in our study, and the duration of storage was very short. Lim et al (9) reported that the motility, viability, morphology, DNA integrity, and mitochondrial potential of human sperm were not affected by 3-month storage in LN 2 vapor (approximately À185 C) compared with storage in LN 2 . They also found no significant difference in the motility and viability of thawed sperm after storage in LN 2 or at 7 cm (approximately À168 C), 12 cm (approximately À167 C), or 17 cm (approximately À161 C) above the surface of LN 2 for 1 week.…”

Section: Discussionmentioning

confidence: 99%

“…However, the temperature of LN 2 vapor is higher than that of LN 2 , which may affect the quality of cryopreserved human sperm (6). Although some studies have reported that there is no obvious difference in sperm quality between LN 2 vapor and LN 2 for human sperm storage, the duration of storage (from 3 days to 3 months) was short (7)(8)(9). These studies also did not evaluate the acrosin activity of preserved sperm, which is affected by cryopreservation (10) and can be considered as a predictor of sperm fertilizing capacity in standard IVF (11,12).…”

mentioning

confidence: 99%