Effect of lipoproteins on cholesterol synthesis in rat Sertoli cells

Maboundou, J.-C.; Fofana, M.; Fresnel, J.; Bocquet, Jean; Goff, D. Le

doi:10.1139/o95-008

Cited by 13 publications

(7 citation statements)

References 29 publications

(25 reference statements)

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“…The base of the Sertoli cell is continually exposed to changing plasma cholesterol levels. The suggestion that Sertoli cells possess Apo B and Apo E receptors for the uptake and degradation of low-density and high-density lipoproteins [47] indicates that the supporting cells could actively regulate supplies of cholesterol within the tubules.…”

Section: Discussionmentioning

confidence: 99%

Expression, Activity, and Subcellular Localization of Testicular Hormone-Sensitive Lipase During Postnatal Development in the Guinea Pig1

Kabbaj

Holm

Vitale

et al. 2001

Biology of Reproduction

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The present work reports on testicular hormone-sensitive lipase (HSL), the biological significance of which has been documented in male fertility. The HSL protein levels and enzymatic activity were measured, respectively, by densitometry of immunoreactive bands in Western blots, performed with antibodies against recombinant rat HSL, and by spectrophotometry in seminiferous tubules (STf) and interstitial tissue (ITf) enriched fractions generated from neonatal, pubertal, and adult guinea pig testes. In addition, HSL was studied in subcellular fractions obtained from STf isolated from adult testes and in epididymal spermatozoa (Spz). A 104-kDa HSL protein was detected in STf and ITf, the expression and activity of which increased with testicular development. Three immunoreactive bands of 104, 110, and 120 kDa were detected in the lysosomal subfraction, and two bands of 104 and 120 kDa were detected in Spz. The HSL activity was positively correlated with free (FC) and esterified (EC) cholesterol ratios in STf and ITf, but not with triglyceride (TG) levels, during testicular development. Immunolabeling localized HSL to elongated spermatids and Sertoli cells, where its distribution was stage-dependent, and within the cells lining the excurrent ducts of the testis. The findings of the 104- and 120-kDa HSL immunoreactive bands and of HSL activity in Spz as well, as the detection of the 104-, 110-, and 120-kDa immunoreactive bands in lysosomes, suggest that part of HSL may originate from germ cells and be imported in Sertoli cells. The HSL protein levels and enzymatic activity in ITf and STf were positively correlated with serum testosterone levels during development. To the best of our knowledge, this study is the first to contribute insights regarding the impact of HSL on FC:EC cholesterol ratios and TG levels in the interstitial tissue and tubules in relation to serum testosterone levels during postnatal development, and regarding the immunolocalization of the enzyme in regions of the male gamete consistent with spermatozoa-oocyte interaction.

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Section: Discussionmentioning

confidence: 99%

Expression, Activity, and Subcellular Localization of Testicular Hormone-Sensitive Lipase During Postnatal Development in the Guinea Pig1

Kabbaj

Holm

Vitale

et al. 2001

Biology of Reproduction

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“…Testosterone via this mechanism increases the uptake of HDL particles, mainly by hepatic cells and testicular Sertoli and Leydig cells (direct binding to SR-B1 receptors), and the excretion of cholesterol from peripheral cells including macrophages (Langer et al, 2002). Additionally, testosterone increases cholesterol transfer through the liver to cholic acid (reverse cholesterol transport; Fofana, Maboundou, Bocquet, & Le Goff, 1996;Langer et al, 2002;Maboundou, Fofana, Fresnel, Bocquet, & Le Goff, 1995). Also, hepatic lipase hydrolyzes phospholipids on the surface of HDL particles allowing the binding of HDL lipids to SR-B1 receptors.…”

Section: Aging and Hdl Cholesterolmentioning

confidence: 99%

Aging Men and Lipids

et al. 2010

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Many theories aim at explaining the mechanisms of aging and death in humans. Decreased levels of androgens, growth hormone, and insulin-like growth factor accompany natural aging in men. Androgens influence the growth and maturation of men in various stages of their life. The action of androgens is performed by binding or not binding to androgen receptors. However, various actions of androgens were clarified after the discovery and genotyping of the androgen receptor. The influence of androgens on the lipid profile was reported by several researchers. This negative influence of androgens in men and the positive influence of estrogens in women are responsible for the higher impact of atherogenesis in men compared with women. In aging men, this negative influence of androgens on the lipid profile is more pronounced. This review considers the influence of age on lipid metabolism in men.

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“…Sertoli cells play a pivotal role in delivering the necessary nutrients such as lipid substrates to the sperm throughout its differentiation. In addition, LDL and VLDL particles cannot pass through the blood-testis barrier, thus their access to the germ cells is excluded [53].…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary L-carnitine on puberty indices in the young breeder rooster

Mohammadi

Sharifi

Sharafi

et al. 2021

Heliyon

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The aim of current study was to investigate the effect of dietary L-Carnitine (LC) in immature roosters on reproductive hormones, lipid profile and testicular histology at the time of maturity. Eighteen 12-wk-old breeder roosters (Ross 308) of similar weights were randomly allocated into 3 dietary treatments (LC-0: basic diet, LC-250: basic diet + 250 mg LC/kg of diet, LC-500: basic diet + 500 mg of LC/kg of diet) in 6 replicates. The feeding program and photoperiod regimen were performed based on ROSS 308 management handbook. Dietary LC supplementation markedly improved testicle weight and testicle index (p < 0.05). Comb height was also affected by LC supplementation (p < 0.05). The testicle weight and index, comb height, and shank lengths improved linearly with increasing levels of dietary LC (p < 0.05). The LC-250 and LC-500 diets significantly improved the number of sertoli cells (NSC), height epithelium seminiferous tubules (HEST), seminiferous tubules diameter (STD), spermiogenesis index (SI) and tubular differentiation index (TDI) of rooster's testis tissue (p < 0.05). The number of seminiferous tubules (NST) was affected by of the amount of LC (p < 0.05). The roosters on the LC-250 mg/kg diet had longer HEST compared to roosters that received the LC-500 mg/kg diet (p < 0.05). Testicular histology parameters increased in a linear and quadratic manner in response to increasing levels of LC (p < 0.05). Dietary LC significantly increased (p < 0.05) plasma concentrations of testosterone, GnRH, LH, FSH and High-Density Lipoprotein (HDL), but reduced the plasma concentration of Low-Density Lipoprotein (LDL). However, no significant differences were observed between LC-250 and LC-500 groups in these parameters. Plasma testosterone, GnRH, LH, LDL and HDL were affected in a linear and quadratic manner in response to increasing levels of LC (p < 0.05). Similarly, FSH increased linearly with increasing dietary LC (p < 0.05). Thus, adding up to 250g of LC per kg of the rooster chicken can improve reproductive hormones, blood lipids and testicular histology parameters at the time of maturity.

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Effect of lipoproteins on cholesterol synthesis in rat Sertoli cells

Cited by 13 publications

References 29 publications

Expression, Activity, and Subcellular Localization of Testicular Hormone-Sensitive Lipase During Postnatal Development in the Guinea Pig1

Expression, Activity, and Subcellular Localization of Testicular Hormone-Sensitive Lipase During Postnatal Development in the Guinea Pig1

Aging Men and Lipids

Effects of dietary L-carnitine on puberty indices in the young breeder rooster

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