Effect of lipid removal on carbon and nitrogen stable isotope ratios in crustacean tissues

Bodin, Nathalie; Loc’h, François Le; Hily, Christian

doi:10.1016/j.jembe.2006.09.008

Cited by 170 publications

(127 citation statements)

References 33 publications

Supporting

Mentioning

104

Contrasting

Unclassified

Order By: Relevance

“…Samples were manually ground to a fine powder and were not lipid-extracted, as shrimp muscle contains low lipid levels. Bodin et al (2007) demonstrated that ␦ 13 C VPDB and ␦ 15 N AIR values in muscle tissue of decapod crustaceans experience minimal changes after solvent treatment; therefore, the use of untreated muscle samples for isotopic analysis has been recommended (Stenroth et al 2006). Samples of ground muscle tissue (900 to 1100 g) were individually packed in tin cups (Elemental Microanalysis Ltd., UK) and organized in 96-well microplates.…”

Section: Sample Pretreatment and Stable Isotope Analysismentioning

confidence: 99%

Application of stable isotope analysis to differentiate shrimp extracted by industrial fishing or produced through aquaculture practices

Gamboa‐Delgado

Molina-PovedaCésar²,

Godínez-Siordia

et al. 2014

Can. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

Carbon and nitrogen stable isotope values were determined in Pacific white shrimp (Litopenaeus vannamei) with the objective of discriminating animals produced through aquaculture practices from those extracted from the wild. Farmed animals were collected at semi-intensive shrimp farms in Mexico and Ecuador. Fisheries-derived shrimps were caught in different fishing areas representing two estuarine systems and four open sea locations in Mexico and Ecuador. Carbon and nitrogen stable isotope values (␦ 13 C VPDB and ␦ 15 N AIR ) allowed clear differentiation of wild from farmed animals. ␦ 13 C VPDB and ␦ 15 N AIR values in shrimps collected in the open sea were isotopically enriched (−16.99‰ and 11.57‰), indicating that these organisms belong to higher trophic levels than farmed animals. ␦ 13 C VPDB and ␦ 15 N AIR values of farmed animals (−19.72‰ and 7.85‰, respectively) partially overlapped with values measured in animals collected in estuaries (−18.46‰ and 5.38‰, respectively). Canonical discriminant analysis showed that when used separately and in conjunction, ␦ 13 C VPDB and ␦ 15 N AIR values were powerful discriminatory variables and demonstrate the viability of isotopic evaluations to distinguish wild-caught shrimps from aquaculture shrimps. Methodological improvements will define a verification tool to support shrimp traceability protocols.

show abstract

Section: Sample Pretreatment and Stable Isotope Analysismentioning

confidence: 99%

Application of stable isotope analysis to differentiate shrimp extracted by industrial fishing or produced through aquaculture practices

Gamboa‐Delgado

Molina-PovedaCésar²,

Godínez-Siordia

et al. 2014

Can. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

show abstract

“…Stable carbon isotope value for wild P. monodon was enriched substantially (about 4.36‰) from farmed P. monodon, while the enrichment for δ 15 N was opposite to wild prawn, farmed prawn was 0.74‰ higher than wild-caught prawn ( Table 1). The bi-plot ( Figure 1) and ANOVA revealed that the farmed P. Monodon were significantly different from wild-caught specimens in terms of δ 13 C (p<0.0001) and δ 15 N (p<0.00034).…”

Section: Resultsmentioning

confidence: 97%

“…Other studies, which have focused on the use of SIA to discriminate between the aquaculture from the wild-caught prawns, have had similar results. For instance, Gamboa-Delgado, Molina-Poveda [18] found that wild-caught Pacific white shrimp had significantly enriched δ 13 C and δ 15 N values when compared to the farmed samples. Ortea & Gallardo [19] found that SIA was able to distinguish the production methods of seven different shrimp species.…”

Section: Resultsmentioning

confidence: 99%

“…The enriched δ 13 C values of the wild-caught P. monodon are linked to their diet comprised of various components available in natural systems, such as benthic diatoms, phytoplankton, macroalgae and seston processed enriched δ 13 C values [20][21][22]. The higher δ 15 N values in the farmed P. Monodon can be attributed to the nitrogen enriched animal-based protein feed [23] consistently used under intense farming. The results of this case study suggest stable carbon and nitrogen analysis could prove to be an effective analytical tool to determine farmed from wild-caught prawns, and has potential for other seafood commodities.…”

Section: Resultsmentioning

confidence: 99%

“…Results were precise (+ 1 SD) to 1% for both %C and %N and 0.3‰ for δ N were expressed in parts per thousand or per mil (‰) deviations from standard values for the two elements and determined through standard analytical procedures [14]. As lipid content in the muscle tissue affects δ 13 C values [15], we employed the widely-used mathematical formula from Post, Layman [16] for normalisation adjustment if the C:N ratio was higher than 3.5: 13 13 3.32 0.99 :…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Untitled

2018

JAMB

View full text Add to dashboard Cite

Determining farmed from wild-caught seafood is important to alleviate public health concerns associated with food safety as well as combatting food fraud. This study applied stable isotope analysis (SIA) to determine provenance of farmed and wildcaught black tiger prawns, P. monodon. Isotopic analysis showed that wild P. monodon were significantly enriched in δ 13 C (p<0.0001) compared to those that were farmed. The results of this case study suggest SIA can be used effectively to differentiate between farmed and wild-caught black tiger prawns, and potentially to determine the production method for other seafood commodities.

show abstract

Amino acid‐specific δ¹⁵N trophic enrichment factors in fish fed with formulated diets varying in protein quantity and quality

Nuche-Pascual¹,

Lazo²,

Ruiz-Cooley

et al. 2018

Ecology and Evolution

View full text Add to dashboard Cite

Compound‐specific isotope analysis (CSIA) of amino acids (AAs) in consumer tissues is a developing technique with wide‐ranging applications for identifying nitrogen (N) sources and estimating animal trophic level. Controlled experiments are essential for determining which dietary conditions influence variability in N stable isotopes (δ15N) trophic enrichment factors in bulk tissue (TEFbulk) and AAs (TEFAA). To date, however, studies have not independently evaluated the effect of protein quantity and quality (digestibility) on TEFs, complicating the application of AA‐δ15N values for estimating trophic levels. We conducted a 98‐d feeding experiment using five formulated isoenergetic feeds prepared with a high‐quality protein source to evaluate the effect of protein quantity and quality on TEFs of liver and muscle tissues of juvenile Pacific yellowtail (Seriola lalandi), a carnivorous fish species. We decreased protein digestibility using well‐established protocols that do not change AA profiles. Growth rates were higher in diets with higher protein content, and isotopic equilibrium was reached for both fish tissues and all treatments. Protein quantity and quality influenced isotope discrimination depending on tissue type and AA. In liver tissue, bulk TEFs showed a limited but significant relationship with protein quality, but did not differ with protein quantity or quality in muscle. None of the pre‐established source AAs (Lys, Met, Phe, and Gly) TEFs varied significantly with protein quantity or quality in liver tissue. However, in muscle tissue, TEFPhe increased significantly with protein content and decreased in response to reduced digestibility, indicating it may not serve as proxy for baseline isotopic values used to calculate trophic level. Among trophic AAs, TEFLeu decreased significantly with increasing protein quantity in liver tissue, while both Leu and Ile TEFs decreased with lower protein digestibility in muscle tissue. Our results indicate that CSIA‐AA in liver tissue provides more robust source and trophic AA‐δ15N values than in muscle.

show abstract

Effect of lipid removal on carbon and nitrogen stable isotope ratios in crustacean tissues

Cited by 170 publications

References 33 publications

Application of stable isotope analysis to differentiate shrimp extracted by industrial fishing or produced through aquaculture practices

Application of stable isotope analysis to differentiate shrimp extracted by industrial fishing or produced through aquaculture practices

Untitled

Amino acid‐specific δ¹⁵N trophic enrichment factors in fish fed with formulated diets varying in protein quantity and quality

Contact Info

Product

Resources

About

Effect of lipid removal on carbon and nitrogen stable isotope ratios in crustacean tissues

Cited by 170 publications

References 33 publications

Application of stable isotope analysis to differentiate shrimp extracted by industrial fishing or produced through aquaculture practices

Application of stable isotope analysis to differentiate shrimp extracted by industrial fishing or produced through aquaculture practices

Untitled

Amino acid‐specific δ15N trophic enrichment factors in fish fed with formulated diets varying in protein quantity and quality

Contact Info

Product

Resources

About

Amino acid‐specific δ¹⁵N trophic enrichment factors in fish fed with formulated diets varying in protein quantity and quality