Effect of lipid peroxidation on membrane permeability of cancer and normal cells subjected to oxidative stress

Paal, Jonas Van der; Neyts, Erik C.; Verlackt, Christof; Bogaerts, Annemie

doi:10.1039/c5sc02311d

Cited by 331 publications

(296 citation statements)

References 70 publications

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“…Initially, plasma membrane-anchored GC-PEG-PpIX molecules activated photoinduced cell membrane damage, causing compromised membrane integrity. According to the literature, ROS can cause peroxidation of membrane lipids which consequently leads to increased membrane permeability and even pore formation [74]. Therefore, extracellular GC-PEG-PpIX NPs were readily internalized into cells and interacted with cytoplasmic membrane-containing organelles to promote cell destruction upon irradiation, which further led to self-enhanced cell influx of PDT agents.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Plasma membrane activatable polymeric nanotheranostics with self-enhanced light-triggered photosensitizer cellular influx for photodynamic cancer therapy

Hao

Jiang

Zhu

et al. 2017

Journal of Controlled Release

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Section: Accepted Manuscriptmentioning

confidence: 99%

Plasma membrane activatable polymeric nanotheranostics with self-enhanced light-triggered photosensitizer cellular influx for photodynamic cancer therapy

Hao

Jiang

Zhu

et al. 2017

Journal of Controlled Release

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“…Our findings also suggest that by controlling α-toc concentration, the stability of biological membranes can be increased. This understanding of how α-tocs affect oxidized membranes is likely to be beneficial for designing new molecules to protect, e.g., skin, against aging 55,56 and in plasma treatment of cancer 48 .…”

Section: Figure 2 the Time Evolution Of The Position Of The Hydroxylmentioning

confidence: 99%

“…The effects these molecules on biological membranes are similar and that has been suggested for being responsible for the observed increase of bilayer thickness and lipid tail order. 25,48,49 Moreover, Issack et al 50 have shown that the free-energy barrier for transferring a water molecule to the center of the bilayer was increased by 6 kJ/mol when 41 mol% cholesterol was present in a DPPC bilayer. This observed significant increase of the free energy barrier to transfer water through bilayer with the presence of cholesterol results in a decrease in water permeability.…”

mentioning

confidence: 99%

Alpha-tocopherol inhibits pore formation in oxidized bilayers

Boonnoy

Karttunen

Wong-ekkabut

2017

Phys. Chem. Chem. Phys.

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ABSTRACT:In biological membranes, alpha-tocopherols (α-toc; vitamin E) protect polyunsaturated lipids from free radicals. Although the interactions of α-toc with nonoxidized lipid bilayers have been studied, their on oxidized bilayers remain unknown. In this study, atomistic molecular dynamics (MD) simulations of oxidized lipid bilayers were performed with varying concentrations of α-toc. Bilayers with 1-palmitoyl-2-lauroyl-sn-glycero-3-phosphocholine (PLPC) lipids and its aldehyde derivatives at 1:1 ratio were studied. Our simulations show that oxidized lipids self-assemble into aggregates with a water pore rapidly developing across the lipid bilayer. The free energy of transporting an α-toc molecule in a lipid bilayer suggests that α-tocs can passively adsorb into the bilayer. When α-toc molecules were present at low concentrations in bilayers containing oxidized lipids, the formation of water pores was slowed down. At high α-toc concentrations, no pores were observed. Based on the simulations, we propose that the mechanism of how α-toc inhibits pore formation in bilayers with oxidized lipids is the following: α-tocs trap the polar groups of the oxidized lipids at the membrane-water interface resulting in a decreased probability for the oxidized lipids to reach contact with the two leaflets and initiate pore formation. This demonstrates that α-toc molecules not only protect the bilayer from oxidation but also help to stabilize the bilayer after lipid peroxidation occurs. These results will help in designing more efficient molecules to protect membranes from oxidative stress.Biological membranes serve as a partition between cells and their environment. Under oxidative stress, unsaturated lipids present in cell membranes may become exposed to attacks by free radicals, that is, oxidation. Oxidation transforms some of the membrane lipids to oxidized ones such as hydroperoxide and aldehyde lipids. 1,2It has also been suggested that internal, that is intra-leaflet, oxidation may be important in altering bilayer properties. 3Lipid peroxidation is an important mechanism of cell membrane damage. [4][5][6] Previous experiments and computer simulations 4,[7][8][9][10][11][12][13][14] have demonstrated how oxidized lipids disturb and deform bilayers. The polar chains in oxidized lipids are energetically unfavorable to stay in the bilayer's interior resulting in the reversal of the polar lipid chain to the bilayer interface. 4,11,15,16 This reversal causes major changes in bilayer properties such as increase of area per lipid, bilayer thinning, decrease of lipid tail order parameter, and increase in water permeability. 4,12,[15][16][17][18] Recently, we performed MD simulations of lipid bilayers with oxidized lipids at high concentrations. Two major oxidized lipid species including hydroperoxide and aldehyde were studied. The results showed that only aldehyde lipids were able to induce pore formation across a PLPC lipid bilayer and cause significant bilayer deformation. 13,15α-toc is well-known as an efficient antioxidant ...

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“…The effects of gamma irradiation on the overall chemical structure of liposomes have been extensively investigated to understand its influence on biological systems (Albertini & Rustichelli 1993;Erdogan et al 2006;Hur Munawar et al 2013;Pamplona 2008;Schneider 2009;Stark 1991;Van der Paal et al 2016). The understanding of such mechanisms is vital, because liposomes represent a good model in the study of the effect of radiation on food, cells as well as sterilization of drug carriers.…”

Section: Introductionmentioning

confidence: 99%

Effect of Gamma Irradiation on the Physical Stability of DPPC Liposomes

Napia

Rahman²,

Hamzah

et al. 2018

JSM

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Effect of lipid peroxidation on membrane permeability of cancer and normal cells subjected to oxidative stress

Abstract: Molecular dynamics simulations suggest that the cholesterol-induced stability of lipid membranes during lipid peroxidation offers an explanation for the observed selectivity of plasma treatments towards cancer cells.

Cited by 331 publications

References 70 publications

Plasma membrane activatable polymeric nanotheranostics with self-enhanced light-triggered photosensitizer cellular influx for photodynamic cancer therapy

Plasma membrane activatable polymeric nanotheranostics with self-enhanced light-triggered photosensitizer cellular influx for photodynamic cancer therapy

Alpha-tocopherol inhibits pore formation in oxidized bilayers

Effect of Gamma Irradiation on the Physical Stability of DPPC Liposomes

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