Effect of Lipid Absorption by Perfluorochemical Emulsions in the Bloodstream and Perspectives of its Clinical Application

Tereshina, E. V.; Afonin, N. I.

doi:10.3109/10731199409138835

Cited by 1 publication

(1 citation statement)

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“…A hypothesis may be drawn that these morphological changes in the cell wall observed with electron microscopy are due to an interaction between PFOB and the cell wall of P. aeruginosa. Perfluorochemicals circulating in the bloodstream have been shown to interact with cholesterol-rich lipoproteins (such as high-density lipoproteins) and the erythrocyte membrane, absorbing cholesterol [20,21]. The outer membrane of gram-negative organisms contains lipopolysaccharides with 6-7 covalently linked fatty acid chains [15].…”

Section: Discussionmentioning

confidence: 99%

Effect of Perfluorooctyl Bromide on Bacterial Growth

Jung

Pendland

2003

Chemotherapy

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Background: Perfluorooctyl bromide (PFOB) is administered directly into the lungs of critically ill patients during partial liquid ventilation. This adjunctive therapy facilitates respiratory support in lung-injured patients and potentially interacts with pathogens in patients with pneumonia. The purpose of this study was to determine the interaction of PFOB with Pseudomonas aeruginosa. Methods: The antimicrobial activity of PFOB against P. aeruginosa was determined using modified time-kill methods. PFOB concentrations of 25, 50, 75, 90 and 99% were studied. Electron microscopy was used to evaluate morphologic changes following PFOB exposure to the organism. Results: Viable counts at baseline were approximately 1 log₁₀ lower when P. aeruginosa was exposed to PFOB compared to controls. Significant bacterial killing occurred over the first 2 h for the 90% (p = 0.023) and 99% (p = 0.045) PFOB concentrations versus control. Electron microscopy demonstrated that PFOB disturbs the bacterial cell wall, and produces vacuolizations within the cell. Conclusions: PFOB kills P. aeruginosa in a concentration-dependent manner through disruption of the cell architecture.

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Section: Discussionmentioning

confidence: 99%