Effect of Linagliptin on the Ratio of Apoptosis Regulators in the Model of Non-Alcoholic Fatty Liver Disease in db/db Mice

Мичурина, С. В.; Cherepanova, M. A.; Ishchenko, I. Yu.; Архипов, С. А.; Климонтов, В. В.; Zavyalov, E. L.

doi:10.1007/s10517-019-04493-1

Cited by 3 publications

(1 citation statement)

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“…Bax, Bid, and Bcl‐2 are activated when apoptosis happens (Tang et al, 2021). Typically, the content of intracellular Bid in the cytoplasm increased, which upregulated the Bax on the mitochondrial membrane and downregulated the Bcl‐2 Michurina et al (2019). Caspase‐9 is known to be activated to form apoptotic bodies, which further activated caspase‐3, a widely distributed effector molecule in the process of apoptotic cell death.…”

Section: Discussionmentioning

confidence: 99%

α‐Pinene inhibits the growth of cervical cancer cells through its proapoptotic activity by regulating the miR‐34a‐5p/Bcl‐2 signaling axis

Huang

Yan

Ding

et al. 2022

Drug Development Research

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Purpose α-pinene was a chemical compound which was extracted from pine needles oil, and it exerted effects on various diseases. However, the effect of α-pinene on cervical cancer had not been reported. The goal of this study was to explore the anti-tumor role of α-pinene.Methods Methyl thiazolyl tetrazolium (MTT) method was used to detect cytotoxicity of α-pinene. Flow cytometry was used to quantify the cell cycle and apoptosis. TUNEL staining was also performed for the revalidation of apoptosis. QRT-PCR and western blot was implemented to detect the expression levels of apoptosis genes and miR-34a-5p. Tumor-bearing nude mouse models was adopted to assess the antitumor action of α-pinene in vivo. ResultsThe results displayed α-pinene restrained proliferation of Hela cells in G1 phase and induced Hela cell apoptosis, which was related to up-regulating expressions of Bax, Bid, Caspase-9, Caspase-3, miR-34a-5p and down-regulating the expression of Bcl-2. Afterwards, α-Pinene could regulate miR-34a-5p/Bcl-2 pathway. Furthermore, α-pinene treatment also induced apoptosis in xenografts tumor models. The uorescence intensity of Bax, Bid, Caspase-9, Caspase-3 increased and uorescence intensity of Bcl-2 decreased.Conclusions Our research demonstrated α-pinene could restrain the development of cervical cancer growth, and it might be an effective chemical compound for therapy of cervical cancer.

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Section: Discussionmentioning

confidence: 99%