Effect of limited access dressing on hydroxyproline and enzymatic antioxidant status in nonhealing chronic ulcers

Honnegowda, Thittamaranahalli Muguregowda; Kumar, Pramod; Udupa, Padmanabha; Rao, Pragna; Bhandary, Siddhartha; Mahato, Krishna Kishore; Sharan, Anurag; Mayya, Shreemathi S

doi:10.4103/0970-0358.138952

Cited by 13 publications

(11 citation statements)

References 23 publications

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“…Chronic wounds often exhibit oxidative stress that slows down the wound-healing process, suppresses collagen deposition and epithelialization and enzymatic antioxidants that play crucial roles in accelerating wound-healing [ 43 ]. We found that L. major infection of mice inhibited significantly ( P <0.05) the antioxidant enzyme activities of superoxide dismutase (SOD) and catalase (CAT) ( Figure 6 ).…”

Section: Resultsmentioning

confidence: 99%

The effects of silver nanoparticles biosynthesized using fig and olive extracts on cutaneousleishmaniasis-induced inflammation in female balb/c mice

et al. 2020

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Leishmaniasis is a group of infectious and non-contagious severe parasitic diseases, caused by protozoans of the Leishmania genus. Natural products characterize a rich source of prospective chemical entities for the development of new effective drugs for neglected diseases. Scientific evaluation of medicinal plants has made it possible to use some metabolites from flavonoids and polyphenols compounds for the treatment of parasitic diseases. Therefore, we aimed in this study to evaluate the protective effect of Silver nanoparticles (Ag-NPs) biosynthesized using Fig and Olive extracts (NFO) against Cutaneous leishmaniasis in female Balb/c mice. A total of 70 mice were used and divided into seven groups. Treatment was initiated when local lesions were apparent, we found Fig and Olive extracts were found to be a good source for the synthesis of (Ag-NPs), their formation was confirmed by color change and stability in solution. Nanoparticles biosynthesized using Fig and Olive extracts induced a reduction in the average size of cutaneous leishmaniasis lesions compared with the untreated mice. Moreover, nanoparticles treatment decreased oxidative stress (LPO, NO), down regulation gene expression levels (TNF-α, IL-1β and BAX) and this antileishmanial activity of nanoparticles was associated with enhanced antioxidant enzyme activities. In addition, histopathological evaluation proved the antileishmanial activity of nanoparticles compared to the positive control. Therefore, we aimed in this study to evaluate the protective effect of silver nanoparticles biosynthesized using Fig and Olive extracts against cutaneous lesions induced by Leishmania major infection through their anti-inflammatory, antioxidant activities and faster clinical efficacy than standard pentavalent antimonial treatment.

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Section: Resultsmentioning

confidence: 99%

The effects of silver nanoparticles biosynthesized using fig and olive extracts on cutaneousleishmaniasis-induced inflammation in female balb/c mice

et al. 2020

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“…Chronic wounds often exhibit oxidative stress that slow down the wound-healing process, suppress collagen deposition and epithelialization and enzymatic antioxidants play crucial role in accelerating wound-healing [ 31 ]. Herein, we found that L. major infection in the mice inhibited significantly ( p < 0.05) the antioxidant enzyme activities of SOD, CAT and GPx ( Figure 6 ).…”

Section: Resultsmentioning

confidence: 99%

Clinical Efficacy Associated with Enhanced Antioxidant Enzyme Activities of Silver Nanoparticles Biosynthesized Using Moringa oleifera Leaf Extract, Against Cutaneous Leishmaniasis in a Murine Model of Leishmania major

El‐Khadragy

Al-Olayan

Metwally

et al. 2018

IJERPH

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Leishmaniasis is one of the most significant vector-borne syndromes of individuals. This parasitic infection can be affected by many species of Leishmania, most of which are zoonotic. Natural products have made and are continuing to make important contributions to the search for new antileishmanial agents. The use of plants in the production assembly of silver nanoparticles has drawn attention because of its rapid, eco-friendly, non-pathogenic, economical protocol and provides a single step technique for the biosynthetic process. Hence, we aimed to biosynthesize silver nanoparticles (Ag-NPs) using Moringa oleifera leaf extract and investigated the antileishmanial activity of these nanoparticles in a murine model of Leishmania major infection. A total of 50 mice were used and divided into five groups—healthy control, infected, infected mice treated with pentostam, infected mice treated with Ag-NPs and infected mice pretreated with Ag-NPs. In the present study, the leaf extract of the plant species Moringa oleifera was found to be a good source for the synthesis of silver nanoparticles, their formation being confirmed by color change and stability in solution. In the present murine model of Leishmania major infection, we found that oral treatment with silver nanoparticles biosynthesized using Moringa oleifera extract resulted in a significant reduction in the average size of leishmaniasis cutaneous lesions compared with untreated mice. Furthermore, the clinical efficacy of Moringa oleifera extract was associated with enhanced antioxidant enzyme activities. In conclusion, treatment with silver nanoparticles biosynthesized using Moringa oleifera extract has higher and faster clinical efficacy than standard pentavalent antimonial treatment, probably by boosting the endogenous antioxidant activity.

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“…Evaluation of the number of blood vessels was done on day 21 after wounding. The wound level of hydroxyproline was measured at days 14 and 21 after wounding [ 66 ].…”

Section: Methodsmentioning

confidence: 99%

“…The concentration of hydroxyproline, as a major component of collagen and a marker of collagen biosynthesis [ 66 ], was measured in the wound tissue samples at days 14 and 21 after wounding using the colorimetric method/kit (Kiazist Co., Hamedan, Iran). The hydroxyproline concentrations were normalized to the amount of sample protein concentrations.…”

Section: Methodsmentioning

confidence: 99%

Acidified Nitrite Accelerates Wound Healing in Type 2 Diabetic Male Rats: A Histological and Stereological Evaluation

et al. 2021

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Impaired skin nitric oxide production contributes to delayed wound healing in type 2 diabetes (T2D). This study aims to determine improved wound healing mechanisms by acidified nitrite (AN) in rats with T2D. Wistar rats were assigned to four subgroups: Untreated control, AN-treated control, untreated diabetes, and AN-treated diabetes. AN was applied daily from day 3 to day 28 after wounding. On days 3, 7, 14, 21, and 28, the wound levels of vascular endothelial growth factor (VEGF) were measured, and histological and stereological evaluations were performed. AN in diabetic rats increased the numerical density of basal cells (1070 ± 15.2 vs. 936.6 ± 37.5/mm3) and epidermal thickness (58.5 ± 3.5 vs. 44.3 ± 3.4 μm) (all p < 0.05); The dermis total volume and numerical density of fibroblasts at days 14, 21, and 28 were also higher (all p < 0.05). The VEGF levels were increased in the treated diabetic wounds at days 7 and 14, as was the total volume of fibrous tissue and hydroxyproline content at days 14 and 21 (all p < 0.05). AN improved diabetic wound healing by accelerating the dermis reconstruction, neovascularization, and collagen deposition.

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Effect of limited access dressing on hydroxyproline and enzymatic antioxidant status in nonhealing chronic ulcers

Cited by 13 publications

References 23 publications

The effects of silver nanoparticles biosynthesized using fig and olive extracts on cutaneousleishmaniasis-induced inflammation in female balb/c mice

The effects of silver nanoparticles biosynthesized using fig and olive extracts on cutaneousleishmaniasis-induced inflammation in female balb/c mice

Clinical Efficacy Associated with Enhanced Antioxidant Enzyme Activities of Silver Nanoparticles Biosynthesized Using Moringa oleifera Leaf Extract, Against Cutaneous Leishmaniasis in a Murine Model of Leishmania major

Acidified Nitrite Accelerates Wound Healing in Type 2 Diabetic Male Rats: A Histological and Stereological Evaluation

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