Effect of lignosulphonates on Vanilla planifolia shoot multiplication, regeneration and metabolism

Abstract

Vanilla planifolia (V. planifolia) is a valuable orchidaceous plant, commonly grown for its pods that are used to produce the flavouring vanilla extract. Here, we evaluated the effect of calcium lignosulphonate (Ca-LIGN) and sodium lignosulphonate (Na-LIGN) on multiplication and regeneration of V. planifolia shoot tip culture. In 150 mg L−1 Ca-LIGN medium, the most number of shoots per explant (5.78 ± 0.63) was successfully obtained. Besides, Ca-LIGN also enhanced the shoot bud and primordial formation rate, as seen under scanning electron microscopy. In contrast, medium containing 150 mg L−1 Na-LIGN recorded the highest average of shoot length (4.72 ± 0.30 cm). Meanwhile, the best growth of root length (1.8 ± 0.32 cm) and root induction (96.67 ± 5.16%) were recorded on the explants treated with 150 mg L−1 Na-LIGN rooting medium. All rooted plantlets successfully acclimatized in the greenhouse (100.00% survival rate). Further biochemical analysis revealed that Ca-LIGN increased the total protein, chlorophyll, sugar, flavonoid and phenolic contents of V. planifolia. Notably, expression of both ribulose-1,5bisphosphate carboxylase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC) genes were also elevated under the treatment of Ca-LIGN, implying a positive role in the photosynthetic process. Taken together, LIGN being an environmental friendly product could be used to enhance the growth and micropropagation of V. planifolia.

λ-Carrageenan promotes plant growth in banana via enhancement of cellular metabolism, nutrient uptake, and cellular homeostasis

“…Previously, plant elicitors, either biotic or abiotic, had been employed to stimulate plant growth for better yield production 9 . This included the usage of elicitors such as carrageenan, chitosan, β-Aminobutyric acid, lignosulfonate and beneficial micro-organisms [9][10][11] . Carrageenan is a naturally occurring high molecular weight polysaccharide, which is extracted from edible red seaweed (Rhodophyta) 11 .…”

Effect of different concentrations of benzylaminopurine for the in vitro propagation of Vanilla tahitensis (J. W. Moore)

“…Con relación a la longitud de raíz evaluada a los 90 d, el tratamiento de 1,75 mg/L de BAP promovió la mayor longitud de las raíces con un promedio 5 cm, mientras que en nuestro laboratorio resultados previos indicaron una longitud radical de 1,74 cm cuando se aplicó la concentración de 1 mg/L. Wan Abdullah et al (2020) aplicaron 1 mg/L de BAP en V. planifolia obteniendo 0,83 ± 0,21 cm de longitud de raíces promedio en el tratamiento control. Con el avance en el conocimiento de las condiciones óptimas de cultivo in vitro de V. tahitensis se abren las posibilidades para desarrollar protocolos eficientes para la micropropagación in vitro de esta orquídea con alta perspectiva en el mercado comercial gourmet debido a sus características específicas.…”

Sodium Lignosulfonate Improves Shoot Growth of Oryza Sativa via Enhancement of Photosynthetic Activity and Reduction of Stress Response

“…In addition, study by Docquier et al 10 suggested that incorporation of CaLS in the medium was able to enhance the nutrient ion availability to the plant, leading to better nutrient ion acquisation and growth performance of orchid, poplar and California redwood. Recent study by Wan Abdullah et al 14 also demonstrated that incorporation of CaLS improved the shoot proliferation rate of in vitro vanilla plant. The increased in vanilla shoot proliferation rate was associated with enhanced chlorophyll content, that contributed to the better photosynthetic activity for plant growth.…”