Effect of light quality and culture medium on somatic embryogenesis of Agave tequilana Weber var. Azul

Abstract

Somatic embryogenesis in Agave tequilanaWeber var. Azul was affected by the interaction between the light regimes applied during the induction phase and the expression phase. When embryogenic calli was exposed to white or red light during the expression phase, an average of two germinated embryos per explant was obtained regardless of the light treatment used for callus induction. Conversely, the highest number of germinated embryos, an average of 18 per explant, was obtained when applying either white or red light during the induction phase and then wide-spectrum light during the expression phase. Culture medium had also a great influence in this process, with embryo germination being reduced by up to 70%, depending on the light treatment, when using Schenk and Hildebrandt (SH) medium instead of Murashige and Skoog (MS) medium.

Production of bioactive phenolic acids and furanocoumarins in in vitro cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. under different light conditions

“…The influence of light quality on the induction and expression of embryogenesis in in vitro cultures of Agave tequilana var. Azul (Rodríguez-Sahagún et al 2011) and Campanula punctata var. rubriflora (Sivanesan et al 2011) and the induction of protocorm-like bodies in in vitro cultures of orchids -Dendrobium officinale (Lin et al 2011) and Oncidium sp.…”

Plant Somatic Embryogenesis: Some Useful Considerations

“…(Cheong & Pooler, 2004). In contrast, the expression and the maturation of embryos of Agave tequilana Weber were successfully achieved when embryos were exposed to red light for 15 days in LOG medium at λ= 630 nm (Rodríguez-Sahagún et al, 2011). Germanà et al (2005) evaluated the effect of light quality in a culture of Citrus clementina Hort.…”

Somatic Embryogenesis in Agave: An Overview

“…The importance of culture media composition in the production of agaves embryos was reported in A. tequilana, and explants produced 95 and 75 % of callus when cultured on MS and SH, respectively (Rodríguez-Sahagún et al 2011). Both media differed in their total ionic strength, nitrogen level, ammonium concentration, and the ammonium-to-nitrate ratio (Rodríguez-Sahagún et al 2011).…”

“…Tissues obtained from a leaf base have been reported for A. victoriae-reginae (Rodríguez-Garay et al 1996), A. vera-cruz (Tejavathi et al 2007), and A. tequilana (Portillo et al 2007;Rodríguez-Sahagún et al 2011).…”