Effect of Light on the Concentration of Adenine Nucleotides in Trichoderma viride

Abstract

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Hyperpolarization and intracellular acidification in Trichoderma viride as a response to illumination

“…The massive discharge of H+ from mitochondria would result in their hyperpolarization which, according to chemiosmotic theory, could be used as a driving force to generate ATP. A doubling in the intracellular level of ATP following illumination has been reported previously (FarkaS et al, 1985;GreSik et al, 1988).…”

“…Measurement of A$ and intracellular p H Our previous observations (FarkaS et al, 1985;GreSik et al, 1988GreSik et al, , 1989 have indicated that the reception of the light signal by dark-growing cells of T. uiride is accompanied by a complex set of physiological and metabolic changes, comprising a transient increase in A$ and an increase in intracellular levels of ATP and CAMP.…”

“…Mycelia were photoinduced as previously described (FarkaS et al, 1985). All operations requiring darkness were done under a red safelight.…”

“…The disturbed charge homeostasis in the cells resulting from the efflux of K + is compensated by H+ discharged into the cytoplasm from light-activated mitochondria, thereby causing the acidification of cytoplasm. The electrochemical gradient for H+ created between the inner membrane of mitochondria and the cytoplasm is used to drive the production of ATP whose intracellular concentration rapidly increases after the onset of illumination (FarkaS et al, 1985;GreSik et al, 1988 …”

Regulation of Glycoside Hydrolase Expression in Trichoderma

“…cAMP was shown to positively influence cellulase gene expression (Dong et al, 1995;Sestak and Farkas, 1993) and a light dependent effect was observed (Farkas et al, 1985). Moreover, GNA3 was found to strongly impact cAMP levels, albeit this influence did not strictly correlate with cellulase levels .…”