Effect of light conditions on anatomical and biochemical aspects of somatic and zygotic embryos of hybrid larch (Larix × marschlinsii)

Aderkas, Patrick von; Teyssier, Caroline; Charpentier, Jean-Paul; Gutmann, M.; Pâques, Luc; Metté, Claire Le; Ader, Kévin; Label, Philippe; Kong, Lisheng; Lelu‐Walter, Marie‐Anne

doi:10.1093/aob/mcu254

Cited by 22 publications

(15 citation statements)

References 45 publications

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“…Consequently, internal ABA concentrations are often orders of magnitude higher in somatic embryos than in zygotic embryos, and the difference is especially pronounced in conifers ( von Aderkas et al, 2001 ). Moreover, the artificial environment induces stress adaptation responses in the developing embryos, resulting ( inter alia ) in increased production of protective secondary metabolites ( von Aderkas et al, 2015 ; Klimaszewska et al, 2016 ). For example, strong upregulation of several genes involved in the biosynthesis of secondary metabolites including flavonols has been detected at the onset of SE maturation in Pinus pinaster ( Morel et al, 2014 ) and Theobroma cacao ( Maximova et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Novel Avenues for Plant Protection: Plant Propagation by Somatic Embryogenesis Enhances Resistance to Insect Feeding

et al. 2018

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Somatic embryogenesis (SE), a clonal propagation method utilizing somatic cells, occurs under conditions that activate plant stress adaptation mechanisms such as production of protective secondary metabolites. Surprisingly, possible differences in susceptibility to insect pests between SE-generated and conventionally cultivated plants have not been previously explored. Here, we recorded frequencies and levels of bark-feeding damage by pine weevils (Hylobius abietis) in two large field trials, consisting of emblings (SE-propagated plants) and seedlings from 50 half-sib Norway spruce (Picea abies) families. We found that emblings were less frequently attacked by pine weevils, and when attacked, they were damaged to a lesser extent than seedlings. Moreover, we detected significant additive genetic variation in damage levels received by plants, indicating a heritable component to differences in resistance to insect herbivory among half-sib families. We present first-time evidence that emblings can be more resistant than seedlings to herbivorous insect damage, thus, SE appears to confer a previously unknown plant protection advantage. This finding indicates novel avenues to explore mechanisms underlying plant resistance and new approaches to develop non-toxic measures against insect pests.

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Section: Introductionmentioning

confidence: 99%

Novel Avenues for Plant Protection: Plant Propagation by Somatic Embryogenesis Enhances Resistance to Insect Feeding

et al. 2018

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“…These were stored at 5 ˚C until further processing. Samples were processed following a published protocol (von Aderkas et al 2015). Briefly, they were washed to remove the glutaraldehyde, then dehydrated in series of increasingly concentrated ethanol solutions, after which they were infiltrated with glycolmethacrylate (Historesin, Reichert-Jung) and embedded for sectioning.…”

Section: Studies Of Seed Developmentmentioning

confidence: 99%

Fungal colonizers and seed loss in lodgepole pine orchards of British Columbia

Bastide

LeBlanc

Kong

et al. 2019

Botany

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Lodgepole pine is an important lumber species in Canada and seed orchards are expected to meet the increased demand for seed. However, seed production has been consistently low in the Okanagan region orchards of British Columbia. To determine whether the fungal microbiome contributes to seed loss, histological and molecular approaches were used. Seed production was studied at seven Okanagan orchards, all outside the natural range of lodgepole pine, and at one near Prince George, within its natural range. Seed losses were highest in the Okanagan, compared with Prince George. The role of fungal colonizers in consuming seed during the last stages of maturation is described. Fungal hyphae were frequently observed at all locations in developing seed, particularly once storage substances accumulated. Fungi identified from host tissues using molecular and morphological techniques included Alternaria, Cladosporium, Fusarium, Penicillium, and Sydowia. The opportunistic foliar pathogen Sydowia polyspora, which is known to have a variable biotrophic status, was detected at most orchards within different host tissues (seeds, needles and conelets), in association with pollen and in the air column. Reduced seed viability observed in Okanagan orchards is most likely due to a combination of factors, including the composition of the fungal microbiome. KeywordsMicrobiome, endophyte, Pinus contorta var. latifolia Engelm. ex S. Watson, Sydowia polyspora (Bref. & Tavel) E. Müll. * Significant differences among sites are indicated by differing letters within a single column (Tukey, α = 0.05).

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“…Associated with different techniques, anatomical analyzes are essential to characterize the cellular alterations that occur in the somatic embryogenesis process. They can help us to understand better the factors that lead somatic cells to resume meristematic characteristics and acquire competence to form somatic embryos; furthermore, histodifferentiation in SE can provide the information necessary to understand the embryogenesis process in plants (Corredoira et al 2006;Moura et al 2008;von Aderkas et al 2015). Histochemical analyzes have allowed a better understanding of the mobilization of reserves and energetic demands during the somatic embryos development (Cangahuala-Inocente et al 2004Moura et al 2010;Rocha et al 2012;Jariteh et al 2015).…”

Section: Somatic Embryogenesis In Annato: Origin and Developmental Stmentioning

confidence: 99%

Somatic Embryogenesis in Annatto (Bixa orellana L.)

Matos

Koehler

Faria

et al. 2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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Our research group has pioneered the work on somatic embryogenesis of Bixa orellana (annatto), and since then we have directed efforts in understanding several aspects of this morphogenic pathway in annatto. Here, we present a synthetic description of such works, emphasizing anatomical analyzes and the characterization of the cellular alterations that occur in the process, and the association of the SERK gene expression and somatic embryogenesis. These results are unprecedented and contribute to a better understanding of the processes involving

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Effect of light conditions on anatomical and biochemical aspects of somatic and zygotic embryos of hybrid larch (Larix × marschlinsii)

Cited by 22 publications

References 45 publications

Novel Avenues for Plant Protection: Plant Propagation by Somatic Embryogenesis Enhances Resistance to Insect Feeding

Novel Avenues for Plant Protection: Plant Propagation by Somatic Embryogenesis Enhances Resistance to Insect Feeding

Fungal colonizers and seed loss in lodgepole pine orchards of British Columbia

Somatic Embryogenesis in Annatto (Bixa orellana L.)

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