Effect of light colour and photoperiod on biomass growth and phycocyanin production by Synechococcus PCC 6715

Klepacz-Smółka, Anna; Pietrzyk, Damian; Szeląg, Rafał; Głuszcz, P.; Daroch, Maurycy; Tang, Jie; Ledakowicz, S.

doi:10.1016/j.biortech.2020.123700

Cited by 39 publications

(30 citation statements)

References 40 publications

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“…Nevertheless, growth rates in all transconjugant strains were reduced compared to WT Synechocystis . The growth rate of T. elongatus under optimal conditions at 45 °C was within the range previously reported for Thermosynechococcaceae and slightly better than that of SynO at 30 °C ( Eberly and Ely, 2012 ; Klepacz-Smolka et al, 2020 ; Liang et al, 2018 ). Notably, T. elongatus failed to grow at 30 °C.…”

Section: Resultssupporting

confidence: 87%

“…However, the energetic costs of maintaining and culturing thermophiles at optimal growth temperatures is significantly higher compared to mesophilic species ( Huang et al, 2017 ). Furthermore, the maximum growth rates of thermophiles as well as the PC content are typically lower ( Klepacz-Smolka et al, 2020 ; Liang et al, 2018 ; Miller and Castenholz, 2000 ). As a result, the highest reported productivity for a thermostable PC from cyanobacteria ( Synechococcus lividus PCC 6715) grown in optimised blue light conditions is ~23 mg L −1 d −1 ( Klepacz-Smolka et al, 2020 ; Liang et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the maximum growth rates of thermophiles as well as the PC content are typically lower ( Klepacz-Smolka et al, 2020 ; Liang et al, 2018 ; Miller and Castenholz, 2000 ). As a result, the highest reported productivity for a thermostable PC from cyanobacteria ( Synechococcus lividus PCC 6715) grown in optimised blue light conditions is ~23 mg L −1 d −1 ( Klepacz-Smolka et al, 2020 ; Liang et al, 2018 ). In comparison, the levels of PC productivity from A. platensis can reach up to 125 mg L −1 d −1 ( Chen et al, 2013a ).…”

Section: Introductionmentioning

confidence: 99%

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Production of thermostable phycocyanin in a mesophilic cyanobacterium

Puzorjov

Dunn

McCormick

2021

Metabolic Engineering Communications

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Phycocyanin (PC) is a soluble phycobiliprotein found within the light-harvesting phycobilisome complex of cyanobacteria and red algae, and is considered a high-value product due to its brilliant blue colour and fluorescent properties. However, commercially available PC has a relatively low temperature stability. Thermophilic species produce more thermostable variants of PC, but are challenging and energetically expensive to cultivate. Here, we show that the PC operon from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 ( cpcBACD ) is functional in the mesophile Synechocystis sp. PCC 6803. Expression of cpcBACD in an ‘Olive’ mutant strain of Synechocystis lacking endogenous PC resulted in high yields of thermostable PC (112 ± 1 mg g −1 DW) comparable to that of endogenous PC in wild-type cells. Heterologous PC also improved the growth of the Olive mutant, which was further supported by evidence of a functional interaction with the endogenous allophycocyanin core of the phycobilisome complex. The thermostability properties of the heterologous PC were comparable to those of PC from T. elongatus , and could be purified from the Olive mutant using a low-cost heat treatment method. Finally, we developed a scalable model to calculate the energetic benefits of producing PC from T. elongatus in Synechocystis cultures. Our model showed that the higher yields and lower cultivation temperatures of Synechocystis resulted in a 3.5-fold increase in energy efficiency compared to T. elongatus , indicating that producing thermostable PC in non-native hosts is a cost-effective strategy for scaling to commercial production.

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Section: Resultssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Production of thermostable phycocyanin in a mesophilic cyanobacterium

Puzorjov

Dunn

McCormick

2021

Metabolic Engineering Communications

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“…When it comes to another thermophilic strain, Thermosynechococcus CL−1 (TCL-1), which was cultivated in small 1–2 L photobioreactors, productivities exceeding those of the BP-1 strain of 3.5 g L −1 d −1 have recently been achieved [ 32 ]. Another study on the 2.6 L helical photobioreactor for cultivation of a high-value product, C-phycocyanin, biosynthesized by ( Thermo ) Synechococcus lividus PCC6715 has achieved similar specific growth rates of 2.054 d −1 [ 33 ]. All these findings indicate that, to date, high productivities in thermophilic cyanobacteria are typically achieved in relatively small volume, high-complexity photobioreactors that require major investment costs.…”

Section: Discussionmentioning

confidence: 99%

Debottlenecking Thermophilic Cyanobacteria Cultivation and Harvesting through the Application of Inner-Light Photobioreactor and Chitosan

Zhang

Chen

Russel

et al. 2021

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Thermophilic cyanobacteria are a low-carbon environmental resource with high potential thanks to their innate temperature tolerance and thermostable pigment, phycocyanin, which enhances light utilisation efficiency and generates a high-value product. However, large-scale cultivation and harvesting have always been bottlenecks in unicellular cyanobacteria cultivation due to their micrometric size. In this study, a 40-litre inner-light photobioreactor (PBR) was designed for scaled-up cultivation of Thermosynechococcus elongatus E542. By analysing light transmission and attenuation in the PBR and describing it via mathematical models, the supply of light energy to the reactor was optimised. It was found that the hyperbolic model describes the light attenuation characteristics of the cyanobacterial culture more accurately than the Lambert–Beer model. The internal illumination mode was applied for strain cultivation and showed a two-fold better growth rate and four-fold higher biomass concentration than the same strain grown in an externally illuminated photobioreactor. Finally, the downstream harvesting process was explored. A mixture of chitosan solutions was used as a flocculant to facilitate biomass collection. The effect of the following parameters on biomass harvesting was analysed: solution concentration, flocculation time and flocculant concentration. The analysis revealed that a 4 mg L−1 chitosan solution is optimal for harvesting the strain. The proposed solutions can improve large-scale cyanobacterial biomass cultivation and processing.

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“…Recent studies have shown that optimal light supply plays a key role in regulating photosynthesis in microalgae and cyanobacteria (Ho et al, 2014;Ho et al, 2018). It is well established that wavelength and intensity of light considerably influence the composition of phycobilisome, and proper adjustment of light can positively affect the energy efficiency as well as C-PC productivity and content (Klepacz-Smółka et al, 2020). Lightdependent variation in the production of PBPs in a cell is known as complementary chromatic adaptation (CCA).…”

Section: Introductionmentioning

confidence: 99%

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Effect of light colour and photoperiod on biomass growth and phycocyanin production by Synechococcus PCC 6715

Cited by 39 publications

References 40 publications

Production of thermostable phycocyanin in a mesophilic cyanobacterium

Production of thermostable phycocyanin in a mesophilic cyanobacterium

Debottlenecking Thermophilic Cyanobacteria Cultivation and Harvesting through the Application of Inner-Light Photobioreactor and Chitosan

Table1.DOCX

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