Effect of lifestyle on age-related mitochondrial protein oxidation in mice cardiac muscle

Padrão, Ana Isabel; Ferreira, Rita; Vitorino, Rui; Alves, Renato M. P.; Figueiredo, Pedro; Duarte, José Alberto; Amado, Francisco

doi:10.1007/s00421-011-2100-3

Cited by 17 publications

(6 citation statements)

References 36 publications

(55 reference statements)

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“…Such age related changes do not only occur in humans but also in rodents and dogs which are often used as animal models to investigate pathomechanisms of cardiovascular diseases. Thus, a decline in mitochondrial function, decreased oxidative phosphorylation, and increased oxidative modification of proteins have been reported as a function of aging [6,7], as well as the presence of post-translational modifications of proteins [8]. It is evident from this knowledge that processes related to aging cannot be neglected in studies targeting the molecular changes occurring in heart diseases.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analyses of age related changes in A.BY/SnJ mouse hearts

et al. 2013

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BackgroundA.BY/SnJ mice are used to study pathological alterations in the heart due to enteroviral infections. Since age is a well-known factor influencing the susceptibility of mice to infection, response to stress and manifestation of cardiovascular diseases, the myocardial proteome of A.BY/SnJ mice aged 1 and 4 months was comparatively studied using two dimensional-differential in-gel electrophoresis (2D-DIGE) and liquid chromatography tandem mass spectrometry (LC-MS/MS).ResultsComplementary analyses by 2D-DIGE and gel-free LC-MS/MS revealed 96 distinct proteins displaying age associated alterations in their levels. Proteins related to protein transport, and transport chain, lipid metabolism and fatty acid transport showed significant changes in 4 months old mouse hearts compared to juvenile hearts. Proteins involved in lipid metabolism and transport were identified at significantly higher levels in older mice and dysregulation of proteins of the respiratory transport chain were observed.ConclusionThe current proteomics study discloses age dependent changes occurring in the hearts already in young mice of the strain A.BY/SnJ. Besides alterations in protein transport, we provide evidence that a decrease of ATP synthase in murine hearts starts already in the first months of life, leading to well-known low expression levels manifested in old mice thereby raising the possibility of reduced energy supply. In the first few months of murine life this seems to be compensated by an increased lipid metabolism. The functional alterations described should be considered during experimental setups in disease related studies.

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Section: Introductionmentioning

confidence: 99%

Proteomic analyses of age related changes in A.BY/SnJ mouse hearts

et al. 2013

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“…; Padrao et al . ). Some contradictory data do exist, with alterations in mitochondrial respiratory complex activities not always being apparent across all tissues in aged animals (Kwong and Sohal, ).…”

Section: Discussionmentioning

confidence: 97%

Effect of reproductive ageing on pregnant mouse uterus and cervix

Patel

Moffatt

Mourmoura

et al. 2017

The Journal of Physiology

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Key points Older pregnant women have a greater risk of operative delivery, still birth and post‐term induction.This suggests that maternal age can influence the timing of birth and processes of parturition.We have found that increasing maternal age in C57BL/6J mice is associated with prolongation of gestation and length of labour.Older pregnant mice also had delayed progesterone withdrawal and impaired myometrial function.Uterine ageing and labour dysfunction should be investigated further in older primigravid women. AbstractAdvanced maternal age (≥35 years) is associated with increased rates of operative delivery, stillbirth and post‐term labour induction. The physiological causes remain uncertain, although impaired myometrial function has been implicated. To investigate the hypothesis that maternal age directly influences successful parturition, we assessed the timing of birth and fetal outcome in pregnant C57BL/6J mice at 3 months (young) and 5 months (intermediate) vs. 8 months (older) of age using infrared video recording. Serum progesterone profiles, myometrium and cervix function, and mitochondrial electron transport chain complex enzymatic activities were also examined. Older pregnant mice had a longer mean gestation and labour duration (P < 0.001), as well as reduced litter size (P < 0.01) vs. 3‐month‐old mice. Older mice did not exhibit the same decline in serum progesterone concentrations as younger mice. Cervical tissues from older mice were more distensible than younger mice (P < 0.05). Oxytocin receptor and connexin‐43 mRNA expression were reduced in the myometrium from 8‐month‐old vs. 3‐month‐old mice (P < 0.05 and P < 0.01 respectively) in tandem with more frequent but shorter duration spontaneous myometrial contractions (P < 0.05) and an attenuated contractile response to oxytocin. Myometrial mitochondrial copy number was reduced in older mice, although there were no age‐induced changes to the enzymatic activities of the mitochondrial electron transport chain complexes. In conclusion, 8‐month‐old mice provide a useful model of reproductive ageing. The present study has identified potential causes of labour dysfunction amenable to investigation in older primigravid women.

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“…However, physiological nitration is not restricted to the embryo heart and was previously described in aging heart mitochondria and in the placenta. In both conditions, the levels of nitration were greatly increased in disease conditions [15,47]. Nitrotyrosine in the normal chorioallantoic membrane chick embryo suggest that tyrosine nitration may be a conserved evolutive trait [48].…”

Section: Discussionmentioning

confidence: 99%

Temporal patterns of tyrosine nitration in embryo heart development

Viera

Radmilovich

Vargas

et al. 2013

Free Radical Biology and Medicine

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Tyrosine nitration is a biomarker for the production of peroxynitrite and other reactive nitrogen species. Nitrotyrosine immunoreactivity is present in many pathological conditions including several cardiac diseases. Because the events observed during heart failure may recapitulate some aspects of development, we tested whether nitrotyrosine is present during normal development of the rat embryo heart and its potential relationship in cardiac remodeling through apoptosis. Nitric oxide (NO) production is highly dynamic during development, but whether peroxynitrite and nitrotyrosine are formed during normal embryonic development has received little attention. Rat embryo hearts exhibited strong nitrotyrosine immunoreactivity in endocardial and myocardial cells of the atria and ventricles from E12 to E18. After E18, nitrotyrosine staining faded and disappeared entirely by birth. Tyrosine nitration in the myocardial tissue coincided with elevated protein expression of nitric oxide synthases (eNOS and iNOS). The immunoreactivity for these NOS isoforms remained elevated even after nitrotyrosine had disappeared. Tyrosine nitration did not correlate with cell death or proliferation of cardiac cells. Analysis of tryptic peptides by MALDI-TOF shows that nitration occurs in actin, myosin, and the mitochondrial ATP synthase alpha chain. These results suggest that reactive nitrogen species are not restricted to pathological conditions but may play a role during normal embryonic development.

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Effect of lifestyle on age-related mitochondrial protein oxidation in mice cardiac muscle

Cited by 17 publications

References 36 publications

Proteomic analyses of age related changes in A.BY/SnJ mouse hearts

Proteomic analyses of age related changes in A.BY/SnJ mouse hearts

Effect of reproductive ageing on pregnant mouse uterus and cervix

Temporal patterns of tyrosine nitration in embryo heart development

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