Effect of LHRH agonists and antagonists in male and female bonnet monkeys (Macaca Radiata)

Use of adult male bonnet monkeys (Macaca radiata) for testing the biological activity of gonadotropin releasing hormone and its analogues

“…However, in an earlier communication we have reported that bonnet monkeys respond very well to GnRH injection. In addition, it was also demonstrated that the increase in serum testosterone is mediated via the increase in serum LH, following GnRH injection (Jagannadha Rao et al, 1985). The present study ex- While control animals received saline, experimental animals received 12.5 pg Ovurelin at 0 h and blood samples collected at specified time intervals and 24 h after the first injection, both groups received 1 pg Ovurelin (indicated by) and serum T determined in blood samples collected 1 h later.…”

“…The antagonist was initially tested in the adult male bonnet monkeys (6-7 years of age weighing 7-8 kg) at four concentrations for agonistic activity using the procedure described for checking the agonists. Following this, the antagonist was checked by testing its ability to block nocturnal surge of testosterone as described earlier (Jagannadha Rao et al, 1985). Briefly a group of monkeys ( n = 6) were checked for nocturnal surge of testosterone on the day prior to experimentation.…”