Effect of LH on testosterone production by foetal rat testes in vitro

Picon, R.; Ktorza, Alain

doi:10.1016/0014-5793(76)80394-8

Cited by 36 publications

(24 citation statements)

References 8 publications

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“…According to the preceeding results, the dose response relationship between LH and cyclic AMP synthesis was tested in an incubation medium containing 0.8 mM Mg and no Ca (fig.4). It is note- worthy that the stimulation of adenylate cyclase activity induced with LH occurs earlier than the stimulation of steroidogenesis described previously [4], so cyclic AMP could be the second messenger in the foetal testis. LH concentrations from 0.05/ag/ ml induced increasing adenylate cyclase activities.…”

Section: Resultsmentioning

confidence: 76%

“…Recent results indicate a more important effect with about 1 tag/ml LH (same origin) on prepubertal rat testes [13]. However concentrations of LH below 0.05/ag/ml were not effective in stimulating adenylate cyclase activity while stimulation of testosterone production could be elicited by 1 ng/ml LH [4]. So the discrepancies previously observed between the concentrations required for the stimulation of testosterone production and enhancement of cyclic AMP concentration [6] were confirmed when adenylate cyclase activity was tested.…”

Section: Resultsmentioning

confidence: 99%

“…The stimulatory effect was weaker in younger testes (table 3). The effect of LH on testosterone secretion was also more pronounced with 20-day-old testes than with younger ones [4]. To obtain a higher stimulation of adenylate cyclase activity and to demonstrate the specificity of the response to LH, it would be necessary to test the hormone on isolated interstitial cells.…”

Section: Resultsmentioning

confidence: 99%

“…Testosterone secretion could be observed in vitro and enhanced with dibutyryl cyclic adenosine-3'-5'-monophosphate (dibutyryl cyclic AMP) [3] and with LH [4]. It has been accepted that in adult testes, LH-induced testosterone biosynthesis was mediated by cyclic AMP [5].…”

Section: Introductionmentioning

confidence: 99%

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Adenylate cyclase in foetal rat testes and its stimulation by LH and NaF

Picon

1976

FEBS Letters

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Section: Resultsmentioning

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Adenylate cyclase in foetal rat testes and its stimulation by LH and NaF

Picon

1976

FEBS Letters

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“…The changes occurring throughout late fetal life in the capacity of the rat testis to synthesize testosterone, and in its ability to respond to gonadotrophin stimulation have been extensively studied using typical organ incubations [4, 5,9,15,16,18,201 …”

mentioning

confidence: 99%

Developmental Changes in in Vitro Testosterone Production by Dispersed Leydig Cells During Fetal Life in Rats

Habert

Brignaschi

1991

Archives of Andrology

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The age-related evolution during fetal days 18.5-21.5 of the capacity of collagenax-dispersed Leydig cells to produce testosterone and to respond to LH or dibutyryl cyclic AMP (dcAMP) was studied in vitro in the rat. When dispersed Leydig cells were incubated in control medium, testosterone secretion by cells from 18.5-day-old fetuses during the first 5 h was 100 and 50% higher than secretion by cells from 20.5-and 21.5-day-old fetuses, respectively. The secretion maximally stimulated by 100 ng/mL LH or stimulated by 1 mM dcAMP was also stronger on day 18.5 than on days 20.5 and 21.5 during the first 3 h of culture. The dose-response curves for LH showed that the ED50 was similar for day 18.5 and 20.5 cells (2 ng/mL LH). During the course of 24-h incubation, the secretion rates also changed with time and fetal age: Between 5 and 24 h of culture basal secretion decreased in day 18.5 cells, rose slightly in day 20.5 cells, and increased sharply in day 21.5 cells; in the same way, in the presence of LH or dcAMP, the secretion by day 18.5 cells decreased faster than that of day 20.5 or 21.5 cells. The basal testosterone secretion of the Leydig cells and its maximum steroidogenic capacity decrease during late fetal life in the rat, and there was no change in the sensitivity to LH during this period. The age-related differences in the variations of the secretion rates as a function of the duration of incubation suggest the existence of an evolution in extragonadotropic regulations during late fetal life.

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Development of Transmembrane Signaling in the Fetal Rat Leydig Cell

Warren

1989

Journal of Andrology

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Gonadotropin binding to the adult Leydig cell activates a GTP binding protein that interacts with adenylate cyclase to increase cAMP production within the cell. The increased production of cAMP stimulates steroidogenesis and leads to an increase in testosterone production and secretion. The fetal Leydig cell responds to LH with an increase in cAMP and testosterone production as early as 15.5 days of gestation, although the specific mechanism of transmembrane signaling has not been characterized. Fetal rat testis cells from 13.5-20.5 days of gestation were treated with dibutyryl cAMP (dbcAMP), cholera toxin, and hCG to determine the onset of steroidogenesis stimulation by activation of each moiety in the transmembrane signaling system of the fetal Leydig cell. Maximal stimulation at each age from 14.5 through 20.5 days of gestation was achieved with 1 mM dbcAMP, 500 ng/ml cholera toxin, or 10 ng/ml hCG. At 13.5 days of gestation, fetal testes did not produce any testosterone. These findings indicate that a cholera toxin-sensitive, stimulatory guanine-nucleotide regulatory protein is functional in the fetal Leydig cell as early as 14.5 days of gestation. The LH receptor becomes functional in the transmembrane signaling system of the fetal Leydig cell at 14.5 days of gestation.

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Effect of LH on testosterone production by foetal rat testes in vitro

Cited by 36 publications

References 8 publications

Adenylate cyclase in foetal rat testes and its stimulation by LH and NaF

Adenylate cyclase in foetal rat testes and its stimulation by LH and NaF

Developmental Changes in in Vitro Testosterone Production by Dispersed Leydig Cells During Fetal Life in Rats

Development of Transmembrane Signaling in the Fetal Rat Leydig Cell

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