Effect of levetiracetam on extracellular amino acid levels in the dorsal hippocampus of rats with temporal lobe epilepsy

Adriana, Pichardo Macías Luz; Alcira, Ramírez Mendiola Blanca; Jatziri, Contreras García Itzel; Zamudio, Sergio; Luis, Chávez Pacheco Juan; Berenice, Sánchez Huerta Karla; Griselda, Mendoza Torreblanca Julieta

doi:10.1016/j.eplepsyres.2018.01.004

Cited by 20 publications

(20 citation statements)

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“…It is important to mention that all our results reflect the semipermanent changes caused by LEV in the neurochemistry of the system, since the recordings were realized four days after the cessation of LEV treatment. This long-lasting effect of LEV has been previously reported for LEV and some other antiepileptic drugs but not for all our electrophysiological parameters studied [ 32 , 36 , 46 , 47 ].…”

Section: Discussionsupporting

confidence: 59%

“…SE was induced by systemic injection of pilocarpine, as previously described [ 35 , 36 , 37 ]. Briefly, animals were pretreated with lithium chloride (127 mg/kg, i.p.…”

Section: Methodsmentioning

confidence: 99%

“…Animals were housed in individual polycarbonate cages and were video monitored to record the occurrence of spontaneous seizures. Video monitoring was performed with four cameras (Steren Model CCTV-970, Mexico City, México), and the recordings were collected during the light period (08:00 to 17:00 h) [ 36 , 39 ]. The videos were analyzed by trained observers using the fast-forward (6×) component of the system.…”

Section: Methodsmentioning

confidence: 99%

“…Two days after the first behavioral spontaneous seizure was observed, an ALZET ® osmotic minipump was implanted subcutaneously for one week to provide subchronic treatment with LEV (300 mg/kg/day). The LEV dose was chosen based on previous experiments in EPI rats [ 32 , 36 ]. LEV treatment via this route has been shown to lead to adequate LEV concentration in blood and proper LEV washout after removing the osmotic minipump [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

“…The LEV dose was chosen based on previous experiments in EPI rats [ 32 , 36 ]. LEV treatment via this route has been shown to lead to adequate LEV concentration in blood and proper LEV washout after removing the osmotic minipump [ 36 ]. To fill the osmotic minipump chambers, LEV was extracted from tablets (Keppra ® ).…”

Section: Methodsmentioning

confidence: 99%

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Levetiracetam Reduced the Basal Excitability of the Dentate Gyrus without Restoring Impaired Synaptic Plasticity in Rats with Temporal Lobe Epilepsy

et al. 2020

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Temporal lobe epilepsy (TLE), the most common type of focal epilepsy, affects learning and memory; these effects are thought to emerge from changes in synaptic plasticity. Levetiracetam (LEV) is a widely used antiepileptic drug that is also associated with the reversal of cognitive dysfunction. The long-lasting effect of LEV treatment and its participation in synaptic plasticity have not been explored in early chronic epilepsy. Therefore, through the measurement of evoked field potentials, this study aimed to comprehensively identify the alterations in the excitability and the short-term (depression/facilitation) and long-term synaptic plasticity (long-term potentiation, LTP) of the dentate gyrus of the hippocampus in a lithium–pilocarpine rat model of TLE, as well as their possible restoration by LEV (1 week; 300 mg/kg/day). TLE increased the population spike (PS) amplitude (input/output curve); interestingly, LEV treatment partially reduced this hyperexcitability. Furthermore, TLE augmented synaptic depression, suppressed paired-pulse facilitation, and reduced PS-LTP; however, LEV did not alleviate such alterations. Conversely, the excitatory postsynaptic potential (EPSP)-LTP of TLE rats was comparable to that of control rats and was decreased by LEV. LEV caused a long-lasting attenuation of basal hyperexcitability but did not restore impaired synaptic plasticity in the early chronic phase of TLE.

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Section: Discussionsupporting

confidence: 59%

“…SE was induced by systemic injection of pilocarpine, as previously described [ 35 , 36 , 37 ]. Briefly, animals were pretreated with lithium chloride (127 mg/kg, i.p.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Levetiracetam Reduced the Basal Excitability of the Dentate Gyrus without Restoring Impaired Synaptic Plasticity in Rats with Temporal Lobe Epilepsy

et al. 2020

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Impaired phase synchronization of motor‐evoked potentials reflects the degree of motor dysfunction in the lesioned human brain

Naros

Machetanz

Leão

et al. 2022

Human Brain Mapping

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The functional corticospinal integrity (CSI) can be indexed by motor‐evoked potentials (MEP) following transcranial magnetic stimulation of the motor cortex. Glial brain tumors in motor‐eloquent areas are frequently disturbing CSI resulting in different degrees of motor dysfunction. However, this is unreliably mirrored by MEP characteristics. In 59 consecutive patients with diffuse glial tumors and 21 healthy controls (CTRL), we investigated the conventional MEP features, that is, resting motor threshold (RMT), amplitudes and latencies. In addition, frequency‐domain MEP features were analyzed to estimate the event‐related spectral perturbation (ERSP), and the induced phase synchronization by intertrial coherence (ITC). The clinical motor status was captured including the Medical Research Council Scale (MRCS), the Grooved Pegboard Test (GPT), and the intake of antiepileptic drugs (AED). Motor function was classified according to MRCS and GPT as no motor deficit (NMD), fine motor deficits (FMD) and gross motor deficits (GMD). CSI was assessed by diffusion‐tensor imaging (DTI). Motor competent subjects (CTRL and NMD) had similar ERSP and ITC values. The presence of a motor deficit (FMD and GMD) was associated with an impairment of high‐frequency ITC (150–300 Hz). GMD and damage to the CSI demonstrated an additional reduction of high‐frequency ERSP (150–300 Hz). GABAergic AED increased ERSP but not ITC. Notably, groups were indistinguishable based on conventional MEP features. Estimating MEP phase synchronization provides information about the corticospinal transmission after transcranial magnetic stimulation and reflects the degree of motor impairment that is not captured by conventional measures.

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Development and validation of a liquid chromatography‐tandem mass spectrometry method with triple‐stage fragmentation to determine levetiracetam in epileptic patient serum and its application in therapeutic drug monitoring

Yin

et al. 2021

J of Separation Science

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Levetiracetam is an antiepileptic drug that is primarily approved by the Food and Drug Administration for the treatment of focal and generalized seizures. This study describes the development and validation of a highly selective and sensitive liquid chromatography‐tandem mass spectrometry method with triple‐stage fragmentation to determine levetiracetam in epileptic patient serum. After simple protein precipitation, the analytes were separated on a short reversed‐phase column (Agilent Poroshell 120 SB‐C18 column, 4.6 × 50 mm, 2.7 μm) using isocratic elution with 25% 0.1% formic acid in water (solvent A) and 75% methanol (solvent B) at a flow rate of 0.8 ml/min. The linear range is 0.5–50 μg/mL (R2 > 0.99). All the validation data, such as lower limit of quantification, linearity, specificity, recoveries, matrix effects, and other parameters, fit the request of biological method validation guidance. Passing–Bablok regression coefficients demonstrated that there is no constant bias and no proportional bias between the liquid chromatography‐tandem mass spectrometry methods with triple‐stage fragmentation and liquid multiple reaction monitoring. Bland–Altman plot showed that the developed liquid chromatography‐tandem mass spectrometry method with triple‐stage fragmentation method is reliable and accurate to determine levetiracetam in human serum.

show abstract

Effect of levetiracetam on extracellular amino acid levels in the dorsal hippocampus of rats with temporal lobe epilepsy

Cited by 20 publications

References 54 publications

Levetiracetam Reduced the Basal Excitability of the Dentate Gyrus without Restoring Impaired Synaptic Plasticity in Rats with Temporal Lobe Epilepsy

Levetiracetam Reduced the Basal Excitability of the Dentate Gyrus without Restoring Impaired Synaptic Plasticity in Rats with Temporal Lobe Epilepsy

Impaired phase synchronization of motor‐evoked potentials reflects the degree of motor dysfunction in the lesioned human brain

Development and validation of a liquid chromatography‐tandem mass spectrometry method with triple‐stage fragmentation to determine levetiracetam in epileptic patient serum and its application in therapeutic drug monitoring

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