Effect of level and duration of dietary n-3 polyunsaturated fatty acid supplementation on the transcriptional regulation of Δ9-desaturase in muscle of beef cattle1

Waters, Sinéad M.; Kelly, Jason P.; O'Boyle, Padraic; Moloney, A.P.; Kenny, David A.

doi:10.2527/jas.2008-1005

Cited by 91 publications

(95 citation statements)

References 32 publications

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“…It might be possible that the tissuespecific effect of n-3 PUFA-supplemented diet on D6d protein expression in our experiments is related to tissue-specific distribution/regulation of the relevant transcription factors. This suggestion is supported by data of the literature that n-3 PUFAenriched diet inhibits SREBP-1c in muscle tissue of bulls (Waters et al, 2009).…”

Section: Discussionsupporting

confidence: 77%

“…One possible reason could be dietary modulation of the rate of n-3 and n-6 fatty acids transfer into the tissues. Waters et al (2009) described that the balance between n-6 and n-3 PUFA in the diet is important for the regulation of SCD through SREBP-1c in muscle of beef cattle. However, this explanation could be applied to C18:2n-6 and C18:3n-3 fatty acids, but not to longer-chain fatty acids.…”

Section: Discussionmentioning

confidence: 99%

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Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls

Herdmann

Nuernberg

Martin

et al. 2010

Animal

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This study investigated the effects of dietary linolenic acid (C18:3n-3) v. linoleic acid (C18:2n-6) on fatty acid composition and protein expression of key lipogenic enzymes, acetyl-CoA carboxylase (ACC), stearoyl-CoA desaturase (SCD) and delta 6 desaturase (Δ6d) in longissimus muscle and subcutaneous adipose tissue of bulls. Supplementation of the diet with C18:3n-3 was accompanied by an increased level of n-3 fatty acids in muscle which resulted in decrease of n-6/n-3 ratio. The diet enriched with n-3 polyunsaturated fatty acids (PUFAs) significantly inhibited SCD protein expression in muscle and subcutaneous adipose tissue, and reduced the Δ6d expression in muscle. There was no significant effect of the diet on ACC protein expression. Inhibition of the Δ6d expression was associated with a decrease in n-6 PUFA level in muscles, whereas repression of SCD protein was related to a lower oleic acid (C18:1 cis-9) content in the adipose tissue. Expression of ACC, SCD and Δ6d proteins was found to be relatively higher in subcutaneous adipose tissue when compared with longissimus muscle. It is suggested that dietary manipulation of fatty acid composition in ruminants is mediated, at least partially, through the regulation of lipogenic enzymes expression and that regulation of the bovine lipogenic enzymes expression is tissue specific.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls

Herdmann

Nuernberg

Martin

et al. 2010

Animal

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“…The strategic supplementation of cattle diets can generate beef with increased conjugated linoleic acid (CLA) and n-3 -E-mail: Sinead.Waters@teagasc.ie polyunsaturated fatty acid (PUFA) concentrations, a lower quantity of fat and a fatty acid profile more compatible with current human dietary recommendations (Moloney et al, 2001). More recently, however, it has been shown that the simultaneous accretion of tissue concentrations of n-3 PUFA and CLA appears to be hampered by the apparent negative effects of n-3 PUFA on tissue CLA synthesis (Waters et al, 2009). Our group has shown that increases in dietary n-3 PUFA supplementation decreased mRNA levels for D-9 desaturase in muscle (Waters et al, 2009).…”

Section: Introductionmentioning

confidence: 80%

“…0 (control), 50 or 100 mM, n 5 16 (8 for fatty acid analysis and 8 for gene expression analysis) for each treatment. The EPA concentrations selected were based on in vivo EPA concentrations recorded in bovine muscle tissue as part of an in vivo experiment at our laboratory (Waters et al, 2009). Following the 24 h incubation, cells and media were harvested.…”

Section: Rna Isolationmentioning

confidence: 99%

“…More recently, however, it has been shown that the simultaneous accretion of tissue concentrations of n-3 PUFA and CLA appears to be hampered by the apparent negative effects of n-3 PUFA on tissue CLA synthesis (Waters et al, 2009). Our group has shown that increases in dietary n-3 PUFA supplementation decreased mRNA levels for D-9 desaturase in muscle (Waters et al, 2009). Furthermore, tissue concentration of the n-3 PUFA, eicosapentaenoic acid (EPA), was found to exhibit a significant negative relationship with D-9 desaturase gene expression.…”

Section: Introductionmentioning

confidence: 99%

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Effect of level of eicosapentaenoic acid on the transcriptional regulation of Δ-9 desaturase using a novel in vitro bovine intramuscular adipocyte cell culture model

Waters

Kenny²,

Killeen³

et al. 2009

Animal

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Ruminant fat is often perceived as having a negative impact on human health; however, the composition of the fat is under complex biochemical control and can be improved through strategic manipulation of the animal's diet. There were two major objectives of this study, namely (i) to develop and validate a primary bovine intramuscular adipocyte cell line and (ii) to examine the effect of eicosapentaenoic acid (EPA) on the transcriptional regulation of D-9 desaturase in vitro using the novel cell line. Intramuscular adipose tissue was obtained from the Musculus longissimus thoracis of a beef heifer. Mature adipocytes were isolated and cultured, and subsequently harvested and evaluated for lipid accumulation and the expression of genes regulating key functional adipocyte protein markers at passages 10, 20 and 30. Isolated cells were shown to accumulate lipid in culture over time. Fatty acid analysis by gas chromatography was carried out at passage 30. Thirteen fatty acids ranging from tetradecanoic acid (C14:0) to the polyunsaturated fatty acid, docosahexaenoic acid (C22:6), were easily detected and measured. High-quality total RNA was isolated from adipocytes and the expression of peroxisome proliferator-activated receptor-g, fatty acid synthase, fatty acid-binding protein-4, adipocyte lipid-binding protein, CD36, D-9 desaturase, sterol regulatory element-binding protein (SREBP), microsomal triglyceride transfer protein and leptin genes were identified by reverse transcriptase-PCR and sequence analysis. Expression of the negative control, liver-specific hepatocyte nuclear factor-1alpha, was not detected. Adipocytes were subsequently incubated in medium containing 0, 50 or 100 mM EPA for 24 h. Increasing the EPA concentration of the culture media led to a linear increase in adipocyte EPA concentration ( P , 0.01). Expression of D-9 desaturase mRNA was decreased five-and seven-fold, respectively, following 50 and 100 mM EPA incubation compared to the control. Gene expression of SREBP-1c was decreased by 6-and 18-fold in cells supplemented with 50 and 100 mM EPA, respectively, compared to the control. Regression analysis showed a negative linear relationship between EPA concentration and the gene expression of both D-9 desaturase ( P , 0.001) and SREBP-1c ( P , 0.001), while a significant positive relationship was observed between D-9 desaturase and SREBP-1c gene expression ( P , 0.001). This is the first report demonstrating that EPA treatment of bovine intramuscular adipocyte cells decreased gene expression of both D-9 desaturase and SREBP-1c in vitro. The bovine adipocyte cell line developed here is an important resource for future studies facilitating less-expensive, rapid screening of research hypotheses and circumventing the limitations associated with the use of experimental animals including cost, inter-animal variation, pre-experimental management and ethics.

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Constraints and potentials for the nutritional modulation of the fatty acid composition of ruminant meat

Bessa

Alves

Santos‐Silva

2015

Euro J Lipid Sci & Tech

144

120

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The meat from ruminants can be a valuable dietary source of rumenic acid (c9,t11-CLA) and of n-3 long chain polyunsaturated fatty acids (n-3 LC-PUFA). Nevertheless, commonly ruminant meats present a fairly low content of both c9,t11-CLA and n-3 LC-PUFA. Most of c9,t11-CLA in meat is derived from the delta-9 desaturation of t11-18:1 catalyzed by stearoyl-CoA desaturase (SCD) and deposited in triacylglycerides (TAG). Thus, optimizing c9,t11-CLA in meat implies: a high substrate supply, a high SCD activity, and a high intramuscular fat (IMF) deposition. High rumen t11-18:1 outflow requires high forage diets which down-regulate SCD activity and IMF deposition. Conversely, as most animals are finished with cereal rich concentrate diets, the SCD activity and IMF deposition are promoted but the rumen outflow of t11-18:1 frequently is replaced by t10-18:1 (t10-shift) which cannot be converted to CLA. Thus the occurrence of the rumen t10-shift is probably the main constraint to c9,t11-CLA enrichment in meat, as is reviewed in detail. The constrains to n-3 LC-PUFA enrichment of ruminant meat are mostly associated to the extensive rumen biohydrogenation of PUFA, the low elongation and desaturation of 18:3n-3 into n-3 LC-PUFA, and the capacity of muscle lipids to incorporate n-3 LC-PUFA. Considering the almost exclusive esterification of n-3 LC-PUFA in membrane phospholipids, the limits to n-3 LC-PUFA incorporation in muscle are discussed considering the amount of and type of available phospholipids as well as the possibility of its esterification in TAG.

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Effect of level and duration of dietary n-3 polyunsaturated fatty acid supplementation on the transcriptional regulation of Δ9-desaturase in muscle of beef cattle1

Cited by 91 publications

References 32 publications

Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls

Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls

Effect of level of eicosapentaenoic acid on the transcriptional regulation of Δ-9 desaturase using a novel in vitro bovine intramuscular adipocyte cell culture model

Constraints and potentials for the nutritional modulation of the fatty acid composition of ruminant meat

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