Effect of Leukemia Inhibiton Factor (LIF) on in vitro maturation and fertilization of matured cattle oocytes

Abstract

Effect of Leukemia Inhibition factor (LIF) on in vitro maturation and fertilization of matured cattle oocytes were tested using frozen thawed semen. Oocytes collected from ovaries of slaughtered cattles were matured at in vitro conditions and fertilized with frozen thawed semen in the fertilization medium with different LIF concentrations Maturation rate and Fertilization rate for five different bulls was determined. A maximum maturation rate was observed at a LIF concentration of 20 μg/mL while the maximum fertilization rate obtained was also at a LIF concentration of 10 μg/ML.