Effect of lesimarin against thioacetamide-induced liver cirrhosis in rat

Abstract

Liver cirrhosis is one of chronic liver diseases with high disability and mortality accompanying fibrosis, hepatocyte damage, and liver dysfunction. In this study, the hepatoprotective and the antifibrotic properties of lesimarin(lecithin-silymarin-Artemisia messerschmitiana Besser(AMB) extract complex at 11:3:6 ratio) on rat hepatic fibrosis induced by thioacetamide (TAA) was investigated. Rats were divided into seven groups: control, lesimarin, TAA, TAA+lesimarin, TAA+lecithin, TAA+silymarin, TAA+AMB. Rats were administered with TAA at a dose of 200 mg/kg body weight intraperitoneally twice a week for three months. Lesimarin, lecithin, silymarin and AMB were administered at a dose of 1.0, 1.0, 0.5, 1.0g/kg body weight orally daily for three months, respectively. TAA administration resulted in hepatic fibrosis, significant decrease in body weight, albumin level and A/G ratio and increase in plasma transaminase, GGT(γ-glutamyltransferase) and ALP(alkaline phosphatase) activities as well as hepatic hydroxyproline content, which were attenuated by lesimarin administration. Lesimarin was found to decrease AST, ALT and GGT, ALP and bilirubin, hydroxyproline levels and increase albumin level and A/G ratio and its effect is more prominent than those of individual constituents. These results suggest this new drug, lesimarin, might be a promising drug to be used for chronic liver diseases.

Effect of Empagliflozin on Thioacetamide-Induced Liver Injury in Rats: Role of AMPK/SIRT-1/HIF-1α Pathway in Halting Liver Fibrosis

“…The rise of non-plasma liver enzyme activities following TAA administration implies their leakage of hepatocytes in response to injury with toxins affecting the integrity and function of liver cells. Also, it signifies an injurious marker for liver damage [ 4 , 25 ]. Furthermore, TAA-induced hyperammonemia is a metabolic change marked by elevated amounts of ammonia, a nitrogen-based molecule that influences the brain’s cognitive function [ 26 ].…”

The involvement of TGF-β1 /FAK/α-SMA pathway in the antifibrotic impact of rice bran oil on thioacetamide-induced liver fibrosis in rats

“…It also inhibits mitochondrial activity eventually leading to cellular damage and hepatocyte necrosis [ 37 , 38 ]. TAA is well-known hepatotoxin produces hardly reversible fibrosis in rodents similar to that of human, for that, it is an ideal model to test potential antifibrotic drugs [ 27 ]. Previous studies showed that TAA induced liver fibrosis through oxidative stress, evidenced by MDA elevation and suppression of GSH and SOD.…”

“…Two weeks of TAA injection (100 mg/kg) three times weekly resulted in a significant increase in the activity of liver marker enzymes (ALT, AST) along with suppression in albumin and total proteins levels. Elevation of the activities of liver transaminases following TAA administration denotes liver cellular leakage and diminution in the structural and functional integrity of liver cells and is thus designated as an index of damage of the liver parenchyma cells [ 27 , 42 ].…”

“…After a one-week of acclimatization, rats were divided into five groups, each with six animals, according to the following scheme: Group 1: the negative control group; rats were injected intraperitoneally ( ip ) with saline three times per week for two successive weeks, Group 2: the positive control (TAA) group; rats were ip injected with TAA (100 mg/kg) three times per week for two successive weeks to provoke liver fibrosis [ 27 ] () with some modification based on our preliminary studies. Group 3 and 4: treatment groups; rats received orally RBO (0.2 and 0.4 mL/kg, orally) [ 28 , 29 ] daily for 2 weeks after 2 weeks of TAA injection.…”

Telmisartan Self-Nanoemulsifying Drug Delivery System, Compared With Standard Telmisartan, More Effectively Improves Hepatic Fibrosis in Rats

“… 35 In rats, TAA administration resulted in significant reductions in body weight, and significant increases in hepatic weight and hepatic/body weight ratio in addition to significant reduction in plasma albumin level. 36 The reduction of body weight is due to the TAA toxic effects and it is considered the most reliable symptom of toxicity in experimental animals. 37 The reduced level of albumin usually shows the severity of the liver dysfunction and it is a reliable index of prognosis.…”