Effect of Leptin Supplementation in Maturation Medium on in vitro Nuclear Maturation and Fertilization of Camel Oocytes (Camelus dromedarius)

Gabr, Sh. A.; Samiah, Sh. M.; Nagy, Wael

doi:10.3923/ajava.2014.713.720

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…Our results showed the same. In this study, The present studythe rates of camel oocyte in vitro maturation showed similar or higher capacity when compared with other studies, meaning that our camel IVM system was appropriate [22][23][24], and analysis revealed thea greater efficiency in obtaining enhanced maturation ratesmatured oocytes from of in vivo-matured oocytes compare to in vitro-sources [Table 1]. Indeed, we confirmed the enhanced capacities for cleavage and blastocyst stage formation of in vivo-matured oocytes.…”

Section: A C C E P T E Dsupporting

confidence: 84%

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Development and pregnancy rates of Camelus dromedarius-cloned embryos derived from in vivo- and in vitro-matured oocytes

Son¹,

Jeong²,

Jeong³

et al. 2022

Anim Biosci

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Objective: The present study evaluated the efficiency of embryo development and clinical pregnancy of somatic cell nuclear transfer (SCNT) embryos using different source-matured oocytes in Camelus dromedarius.Methods: Camelus dromedarius embryos were produced by SCNT using in vivo-and in vitromatured oocytes. In vitro embryo developmental capacity of reconstructed embryos was evaluated. To confirm the efficiency of pregnancy and live birth rates, a total of 72 blastocysts using in vitromatured oocytes transferred into 45 surrogates and 95 blastocysts using in vivo-matured oocytes were transferred into 62 surrogates by transvaginal method. Results:The collected oocytes derived from ovum pick up (OPU) showed higher maturation potential into metaphase II oocytes than oocytes from the slaughterhouse. The competence of cleavage, and blastocyst were also significantly higher in in vivo-matured oocytes than in vitro-matured oocytes.After embryo transfer, 11 pregnant and 10 live births were confirmed in in vivo-matured oocytes group, and 2 pregnant and 1 live birth were confirmed in in vitro-matured oocytes group. Furthermore, blastocysts produced by in vivo-matured oocytes resulted in significantly higher early pregnancy and live birth rates than in vitro-matured oocytes. Conclusion:In this study, SCNT embryos using in vivo-and in vitro-matured camel oocytes were successfully developed, and pregnancy was established in recipient camels. We also confirmed that in vivo-matured oocytes improved the development of embryos and the pregnancy capacity using the blastocyst embryo transfer method.

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Section: A C C E P T E Dsupporting

confidence: 84%

“…These results indicated that it is essential to choose an efficient oocyte maturation method to obtain stable results in the production of live births using SCNT. However, the oocyte maturation rate of camels was lower than that of other species after in vitro maturation, and studies were insufficient [22][23][24].…”

Section: Discussionmentioning

confidence: 99%

Development and pregnancy rates of Camelus dromedarius-cloned embryos derived from in vivo- and in vitro-matured oocytes

Son¹,

Jeong²,

Jeong³

et al. 2022

Anim Biosci

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“…However, the low reproductive performance of camels is considered an important factor affecting camel populations. Employing in vitro embryo production (IVP) technologies is an efficient means of propagating genetically superior camels; however, compared to other livestock species, camel oocyte in vitro maturation (IVM) has been reported to be inefficient [1,2,3,4]. One of the main contributing factors is the limited availability of oocytes because the majority of the slaughtered animals are either old, culled for infertility, or very young and have not attained maturity; in addition, there is no standardized protocol for IVP in camels [3, 5].…”

mentioning

confidence: 99%

Effects of all-trans retinoic acid on the in vitro maturation of camel (Camelus dromedarius) cumulus-oocyte complexes

Saadeldin

Swelum

Elsafadi

et al. 2019

Journal of Reproduction and Development

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All- trans retinoic acid (RA) is a metabolite of vitamin A and has pleiotropic actions on many different biological processes, including cell growth and differentiation, and is involved in different aspects of fertility and developmental biology. In the current study, we investigated the effects of RA on camel ( Camelus dromedarius ) cumulus-oocyte complex in vitro maturation (IVM). IVM medium was supplemented with 0, 10, 20, and 40 µM RA. Application of 20 µM RA significantly reduced the proportion of degenerated oocytes and significantly improved oocyte meiosis and first polar body extrusion compared to the control and other experimental groups. Retinoic acid significantly reduced the mRNA transcript levels of apoptosis-related genes, including BAX and P53 , and reduced the BAX/BCL2 ratio. In addition, RA significantly reduced the expression of the Transforming growth factor beta (TGFβ) pathway-related transcripts associated with the actin cytoskeleton, ACTA2 and TAGLN ; however, RA increased TGFβ expression in cumulus cells. The small molecule SB-431542 inhibits the TGFβ pathway by inhibiting the activity of activin receptor-like kinases (ALK-4, ALK-5, and ALK-7); however, combined supplementation with RA during IVM compensated for the inhibitory effect of SB-431542 on cumulus expansion, oocyte meiosis I, and first polar body extrusion in activated oocytes. The current study shows the beneficial effects of RA on camel oocyte IVM and provides a model to study the multifunctional mechanisms involved in cumulus expansion and oocyte meiosis, particularly those involved in the TGFβ pathway.

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“…It was also observed that addition of 10 ng/mL leptin in pig oocyte maturation caused significantly higher oocyte maturation rates [17,18]. It was reported that 20 ng/mL leptin enhanced buffalo and camel oocyte maturation rates as well as increasing cleavage and in vitro embryo production [19,20]. Addition of 10 ng/mL leptin to IVM medium improves buffalo oocytes nuclear maturation [21].…”

Section: Ivm With Different Concentrations Of Leptinmentioning

confidence: 99%

Effect of supplementing additives in leptin-enriched maturation medium during in vitro maturation and vitrification of goat oocytes

Talukdar¹,

Biswas²,

Kadirvel³

et al. 2020

Turk J Vet Anim Sci

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The present study aimed to evaluate the effect of supplementing epidermal growth factor (EGF), insulin-like growth factor-I (IGF-I), fetal bovine serum (FBS), and bovine serum albumin (BSA) in different combinations in a maturation medium (MM) comprising TCM-199 + 5 µg/mL 17-β estradiol + 5 µg/mL ovine-luteinizing hormone (oLH) + 5 µg/mL pure follicle-stimulating hormone (pFSH) + 100 µM/mL cysteamine containing the best concentration of leptin among 10/20/25/30 ng/mL on in vitro maturation (IVM) and subsequent vitrification of matured goat oocytes. The IVM of oocytes was carried out in a CO 2 incubator for 27 h at 38.5 °C. The percentage of oocytes with cumulus expansion and polar body extrusion was found to be highest in 25 ng/mL leptin at 47.37% and 15.63%; rates of 66.67% and 28.57% were obtained when supplemented with 50 ng/mL IGF-I + 10 ng/mL EGF in medium containing 25 ng/mL leptin as compared to the other media. The in vitro matured oocytes were vitrified in 5 M ethylene glycol + 5 M propylene glycol with 0.5 M sucrose in TCM-199 + 20% FBS using liquid nitrogen. The highest percentage of morphologically intact in vitro matured oocytes following vitrification was obtained in medium containing 25 ng/mL leptin (89.29%). It was concluded that addition of IGF-I + EGF with 25 ng/mL leptin in MM yielded a higher rate of maturation of oocytes, and supplementation of 25 ng/mL leptin in MM resulted in a higher rate of morphologically intact vitrified oocytes, indicating the efficiency of leptin for cryotolerance.

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Effect of Leptin Supplementation in Maturation Medium on in vitro Nuclear Maturation and Fertilization of Camel Oocytes (Camelus dromedarius)

Cited by 7 publications

References 29 publications

Development and pregnancy rates of Camelus dromedarius-cloned embryos derived from in vivo- and in vitro-matured oocytes

Development and pregnancy rates of Camelus dromedarius-cloned embryos derived from in vivo- and in vitro-matured oocytes

Effects of all<i>-trans</i> retinoic acid on the <i>in vitro</i> maturation of camel (<i>Camelus dromedarius</i>) cumulus-oocyte complexes

Effect of supplementing additives in leptin-enriched maturation medium during in vitro maturation and vitrification of goat oocytes

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