Effect of length of utilization on fungi colonizing plant roots, rhizosphere and seeds of fodder galega (Galega orientalis Lam.)

Abstract

The aim of the study was to determine the effect of the length of the fodder galega utilization period on species composition and number of fungi isolated from rhizosphere, rhizoplane and from seeds. Fodder galega plants used for the research were cultivated for the following time length: I-18-19-years, II-8-9-years, III-4-5-years, IV-3-4-years. Analyses were conducted in 2009-2010. In total 953 colonies of fungi were isolated from roots including 476 colonies in 2009 and 477 in 2010. Cylindrocarpon destructans was the most frequently isolated species (18.5%) in the first year of the study, whereas Trichoderma koningii-was the most often isolated fungus in the second year (26.7%). More than 3.000 isolates representing 43 species were isolated from soil. Penicillium spp., T. viride and T. koningii were the most frequently isolated fungi in both years of the study. The highest number of fungi was isolated from roots and from soil in combinations with short period of fodder galega utilization. Ten species of fungi were identified on seeds. Alternaria alternata, Aureobasidium pullulans, Epicoccum purpurascens, Cladosporium herbarum, Stemphylium botryorum, T. koningii and Penicillium spp. were the most often isolated fungi.