Effect of lecithin on the development of experimental atherosclerosis in rabbits

Juźwiak, S.; Wójcicki, J; Machoy-Mokrzyńska, Anna; Samochowiec, L; Biaecka, M.; Juzyszyn, Zygmunt; Barcew-Wiszniewska, B; Skowron, J; Roźéwicka, L; Kadłubowska, D

doi:10.1016/s0944-7113(96)80042-0

Cited by 23 publications

(26 citation statements)

References 24 publications

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“…1-5), suggesting that the effects of quercetin on macrophages might be operating in vivo and could explain at least some of the atheroprotective activities of this flavonoid. Our results are in line with previous reports showing atheroprotective effects of oral quercetin in various animal models (Hayek et al, 1997;Auger et al, 2005;Juźwiak et al, 2005;Leckey et al, 2010;Loke et al, 2010;Kleemann et al, 2011) and further extend them by demonstrating a significant level of protection after intraperitoneal chronic administration. Furthermore, we demonstrated reduced inflammatory macrophage and T cell infiltrate in the atheromatous plaque (Fig.…”

supporting

confidence: 93%

“…Several epidemiological (Hertog et al, 1993(Hertog et al, , 1995 and clinical (Edwards et al, 2007;Egert et al, 2009) studies support the cardioprotective effects of this flavonoid in humans. Moreover, administration of quercetin to rabbits (Juźwiak et al, 2005), hamsters (Auger et al, 2005), and mice (Hayek et al, 1997;Leckey et al, 2010;Loke et al, 2010;Kleemann et al, 2011) inhibits atherosclerosis development, which is the underlying cause of CVD. Cellular and molecular investigations further support quercetin's bioactivity in atherosclerosis and suggest that it acts through antioxidant/cytoprotective and anti-inflammatory mechanisms.…”

Section: Introductionmentioning

confidence: 99%

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Proatherogenic Macrophage Activities Are Targeted by the Flavonoid Quercetin

Lara-Guzmán

Tabares-Guevara

León-Varela

et al. 2012

J Pharmacol Exp Ther

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Many studies have demonstrated that the flavonoid quercetin protects against cardiovascular disease (CVD) and related risk factors. Atherosclerosis, the underlying cause of CVD, is also attenuated by oral quercetin administration in animal models. Although macrophages are key players during fatty streak formation and plaque progression and aggravation, little is known about the effects of quercetin on atherogenic macrophages. Here, we report that primary bone marrow-derived macrophages internalized less oxidized low-density lipoprotein (ox-LDL) and accumulated less intracellular cholesterol in the presence of quercetin. This reduction of foam cell formation correlated with reduced surface expression of the oxLDL receptor CD36. Quercetin also targeted the lipopolysaccharidedependent, oxLDL-independent pathway of lipid droplet formation in macrophages. In oxLDL-stimulated macrophages, quercetin inhibited reactive oxygen species production and interleukin (IL)-6 secretion. In a system that evaluated cholesterol crystal-induced IL-1␤ secretion via nucleotide-binding domain and leucine-rich repeat containing protein 3 inflammasome activation, quercetin also exhibited an inhibitory effect. Dyslipidemic apolipoprotein E-deficient mice chronically treated with intraperitoneal quercetin injections had smaller atheromatous lesions, reduced lipid deposition, and less macrophage and T cell inflammatory infiltrate in the aortic roots than vehicle-treated animals. Serum levels of total cholesterol and the lipid peroxidation product malondialdehyde were also reduced in these mice. Our results demonstrate that quercetin interferes with both key proatherogenic activities of macrophages, namely foam cell formation and pro-oxidant/proinflammatory responses, and these effects may explain the atheroprotective properties of this common flavonoid.

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supporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Proatherogenic Macrophage Activities Are Targeted by the Flavonoid Quercetin

Lara-Guzmán

Tabares-Guevara

León-Varela

et al. 2012

J Pharmacol Exp Ther

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“…Results of the chemical analysis of T. leontopetaloides extract showed the presence of polyphenols, alkaloid and saponins. Several studies have demonstrated the beneficial effects of polyphenols (Juźwiak et al, 2005;Andreadou et al, 2006) and saponins (Francis et al, 2002;Astuti et al, 2009;Afrose et al, 2010) on the reduction of cholesterol. In this study the rats fed with high fat diet associated with aqueous tubers extract loss the weight and rats receiving high fat diet only gained the weight.…”

Section: Discussionmentioning

confidence: 99%

Antihyperlipidemic and hypolipidemic properties of Tacca leontopetaloides (L.) Kuntze (Dioscoreales: Dioscoreaceae) tuber’s aqueous extracts in the rats

Aïssatou¹,

Metsagang²,

Sokeng³

et al. 2017

Braz. J. Biol. Sci.

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Abstract. Tubers of Tacca leontopetaloides (L.) Kuntze (Dioscoreales: Dioscoreaceae) play an important role in food substitution during the periods of food shortage in occidental and central Africa. It is also used in traditional medicine for the treatment of the diarrhea, dysentery, stomach evil, viral hepatitis and the infection of guinea worn. This study attempted to evaluate the antihyperlipidemic and hypolipidemic properties of aqueous extracts of the tubers of T. leontopetaloides in rats. For this experimentation, two tests were done: antihyperlipidemic and hypolipidemic tests. Hyperlipidemia was induced in rats with High Fat Diet containing 300 g of egg yolk, 2 g of cholesterol, 250 g of coconut oil and 50 g of soya oil. The group of rats on which the antihyperlipidemic test was done was fed with High Fat Diet and supplemented with T. leontopetaloides aqueous extract at 27.34 mg/kg, and 54.68 mg/kg; after 21 days of experimentation, the different groups of rats were sacrificed. The lipid profile and some biochemical parameters were evaluated. Organs like kidneys, liver and stomach were taken for histopathological evaluation. For the hypolipidemic test, after three weeks of induction of hyperlipidemia, the diet was changed to normal diet and aqueous extract of tubers was given to rats during 21 days at doses of 3.41 mg/kg and 13.67 mg/kg. The animals were sacrificed after 21 days of experimentation. The lipid profile, some biochemical parameters and histopathology of organs were evaluated. The antihyperlipidemic and hypolipidemic tests of aqueous extract of the tubers of T. leontopetaloides induced reduction in total cholesterol, triglyceride, LDL-cholesterol and an increase in HDL-cholesterol significantly (p < 0.05); decreasing activities of ALAT and ASAT enzymes, levels of creatinin remained no changed. Histopathological study revealed that extracts did not repair the destructions of liver cells and glomerules of kidneys caused by High Fat Diet. Aqueous extract of tubers of T. leontopetaloides exhibits antihyperlipidemic and hypolipidemic activities; hence it could be important in the management of cardiovascular diseases.

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“…Epidemiological (22) and clinical studies (23) suggest that there is an inverse correlation between flavonoid supplementation and cardiovascular risk, and numerous clinical and animal studies have reported the anti-inflammatory and anti-oxidative functions of quercetin (23)(24)(25)(26)(27)(28)(29). Furthermore, quercetin alleviates AS development in rabbits (30) and mice (31).…”

Section: Introductionmentioning

confidence: 99%

Quercetin alleviates cell apoptosis and inflammation via the ER stress pathway in vascular endothelial cells cultured in high concentrations of glucosamine

Cai

Bao

Ding

et al. 2016

Molecular Medicine Reports

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Abstract.Glucosamine is a possible cause of vascular endothelial injury in the initial stages of atherosclerosis, through endoplasmic reticulum (ER) stress resulting in fatty streaks in the vascular wall. Quercetin is an anti-diabetic and cardiovascular protective agent that has previously been demonstrated to reduce ER stress in human umbilical vein endothelial cells (HUVECs). The present study aimed to investigate whether quercetin prevents glucosamine-induced apoptosis and inflammation via ER stress pathway in HUVECs. The effect of quercetin on cell viability, apoptosis, and protein expression levels of inflammatory cytokines and ER stress markers was investigated in glucosamine-supplemented HUVECs. Quercetin was demonstrated to protect against glucosamine-induced apoptosis, improved cell viability, and inhibited expression of pro-inflammatory factors and endothelin-1. Quercetin treatment also reduced the expression levels of glucose-regulated protein 78, phosphorylated protein kinase-like ER kinase, phosphorylated c-Jun N-terminal kinase and C/EBP homologous protein. In conclusion, quercetin may have auxiliary therapeutic potential against glucosamine-induced cell apoptosis and inflammation, which may be partially due to alleviation of ER stress. IntroductionDiabetes is a 'coronary heart disease (CHD) equivalent' disease, as epidemiological data has previously demonstrated that patients with diabetes without any prior evidence of CHD are at greater risk of death from CHD than nondiabetic patients with prior evidence of CHD (1,2). Atherosclerosis (AS) is a disease of arterial lipid deposition leading to a number of biological responses, including a chronic, macrophage-dominated inflammatory reaction (3), and is the pathological basis of CHD. The hexosamine biosynthesis pathway (HBP), a normal pathway for glucose metabolism, is activated excessively in patients with diabetes, resulting in increased cellular glucosamine (4). Endoplasmic reticulum (ER) is a membranous network to synthesize, modify, fold and assemble proteins. When the ER function, particularly folding capacity, is challenged, the unfolded protein response (UPR) is executed as a protective mechanism in ER. Failure of this mechanism to fold newly synthesized proteins shows unique damage to the cell and is termed ʻER stressʼ (5,6). Numerous studies have highlighted that ER stress may link hyperglycemia to AS (4,7,8). Important ER stress markers, including protein kinase-like ER kinase (PERK), glucose regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP), were expressed in the arterial wall of streptozotocin-induced hyperglycemic apolipoprotein (apoE)-deficient mice (4). Glucosamine levels and the expression of GRP78 were increased following hyperglycemia and prior to the early stages of fatty streak formation in aortic endothelial cells of hyperglycemic apoE -/-mice (7). In vitro studies have demonstrated that incubation of hepatic cells (9) and adipocytes (10) with 5 mM glucosamine resulted in lipid accumulation, impaired insulin-stimul...

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Effect of lecithin on the development of experimental atherosclerosis in rabbits

Cited by 23 publications

References 24 publications

Proatherogenic Macrophage Activities Are Targeted by the Flavonoid Quercetin

Proatherogenic Macrophage Activities Are Targeted by the Flavonoid Quercetin

Antihyperlipidemic and hypolipidemic properties of Tacca leontopetaloides (L.) Kuntze (Dioscoreales: Dioscoreaceae) tuber’s aqueous extracts in the rats

Quercetin alleviates cell apoptosis and inflammation via the ER stress pathway in vascular endothelial cells cultured in high concentrations of glucosamine

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