Abstract:The present study was conducted to investigate the vegetative propagation of Sansevieria trifasciata under ex vitro conditions. The experiment was conducted at the net house to select the best leaf segment among apical, middle and bottom portions of leaves and also to evaluate their performance in different potting media. Leaf segments were taken from apical, middle and bottom portions and planted in soil: compost at 1:0, soil: compost at 1:1 and soil: compost at 1:2 ratios. After 90 days, number of shoots pe… Show more
“…7 f–h). This last phase of our micropropagation protocol, an exogenous auxin-free rooting, contrast with those of Hematharshini and Seran 60 and Simbolon 61 , who used 0.5 mg·Lˉ 1 of NAA and 2.0 mg·L -1 of BAP to produce shoots and roots, respectively, and those of Kaur 25 , who used high doses of IBA (5.0–10.0 mg·L -1 ), and Sarmast et al 22 and Wahyuningsih et al 62 , who immersed shoots for 3 s in an IBA solution (2000 mg·L -1 ), to induce rooting. Figure 7 Rooting phase during micropropagation of cvs Lorentii and Hahnii of Sansevieria trifasciata.…”
Sansevieria trifasciata is used as an indoor plant, in traditional medicine and as a fiber source. Here we characterized fibers of two of varieties of S. trifasciata, Lorentii and Hahnii, and report a protocol for their propagation based on indirect shoot organogenesis. Structural and ribbon fibers were scattered within leaf parenchyma when viewed with confocal laser scanning microscopy. Chemical analysis of the fibers by mass spectrometry and high-performance chromatography revealed higher contents of cellulose and xylose in Lorentii than in Hahnii and significant differences for total lignin between both. A protocol for de novo shoot production was then developed using leaf explants. Time-course histological analyses showed that the first events of transdifferentiation were triggered preferentially in cells surrounding fibers and vascular bundles. Callogenesis and shoot performances were quantified for both varieties, and 2,4-D at 2 and 3 mg·L-1 yielded the best results for primary calli induction and fresh calli mass. The length, number, and mass of shoots produced did not differ significantly between the two cultivars. The fast morphogenic response of S. trifasciata to in vitro culture may be useful for mass propagation or other biotechnological purposes such as metabolite production.
“…7 f–h). This last phase of our micropropagation protocol, an exogenous auxin-free rooting, contrast with those of Hematharshini and Seran 60 and Simbolon 61 , who used 0.5 mg·Lˉ 1 of NAA and 2.0 mg·L -1 of BAP to produce shoots and roots, respectively, and those of Kaur 25 , who used high doses of IBA (5.0–10.0 mg·L -1 ), and Sarmast et al 22 and Wahyuningsih et al 62 , who immersed shoots for 3 s in an IBA solution (2000 mg·L -1 ), to induce rooting. Figure 7 Rooting phase during micropropagation of cvs Lorentii and Hahnii of Sansevieria trifasciata.…”
Sansevieria trifasciata is used as an indoor plant, in traditional medicine and as a fiber source. Here we characterized fibers of two of varieties of S. trifasciata, Lorentii and Hahnii, and report a protocol for their propagation based on indirect shoot organogenesis. Structural and ribbon fibers were scattered within leaf parenchyma when viewed with confocal laser scanning microscopy. Chemical analysis of the fibers by mass spectrometry and high-performance chromatography revealed higher contents of cellulose and xylose in Lorentii than in Hahnii and significant differences for total lignin between both. A protocol for de novo shoot production was then developed using leaf explants. Time-course histological analyses showed that the first events of transdifferentiation were triggered preferentially in cells surrounding fibers and vascular bundles. Callogenesis and shoot performances were quantified for both varieties, and 2,4-D at 2 and 3 mg·L-1 yielded the best results for primary calli induction and fresh calli mass. The length, number, and mass of shoots produced did not differ significantly between the two cultivars. The fast morphogenic response of S. trifasciata to in vitro culture may be useful for mass propagation or other biotechnological purposes such as metabolite production.
“…The softwood cuttings of Henckelia Royal Queen were collected from parent plant stock, Royal Botanical Garden, Peradeniya. Potting medium was a mixture of sand and compost at 1:1 ratio (Hematharshini and Seran, 2019) which was sterilized by autoclaving and filled the pots (9.0 cm height and 11.0 cm diameter) by leaving 1.0 cm from the top of pots. At the time of planting, the cuttings were treated with IBA (Indole Butyric Acid) and planted to the depth of 2.0-3.0 cm in the center of the pots and placed inside the plant propagators to accelerate the rooting of cuttings.…”
An experiment was carried out to determine the effect of pinching practice and different concentrations of Paclobutrazol (PBZ) on the flowering of Henckelia Royal Queen plants.Completely randomized design with eight treatment combinations of pinching or non-pinching and different concentrations of Paclobutrazol (0, 15, 30and 60 ppm) soil drenchwas usedwith eight replicates for each treatment. Pinching was practiced twice at two weeks intervals. Flowering parameters viz. number of flower buds, days taken to flowering, number of flowers, pedicel length, corolla length and corolla width were measured at two weeks intervals from 9thweek after the treatment application. Results disclosed that plants showed better flowering performances (P<0.05) with increasing concentrations of PBZ, but degree of leaf deformation was increased. Therefore, the application of 15 ppm PBZ with pinching showed better to obtain dwarf Henckelia Royal Queen plants with improved flowering quality as commercial potted plants.
Bangladesh J. Sci. Ind. Res.56(3), 177-184, 2021
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