Effect of laser (λ 660 nm) and LED (λ 630 nm) photobiomodulation on formocresol-induced oral ulcers: a clinical and histological study on rodents

Abstract

The aim of this study was to evaluate, clinically and histologically, the influence of laser and LED photobiomodulation in the healing of formocresol-induced oral mucosa ulcers of rats. We used 60 Wistar rats in which oral ulcers were induced on the gingiva of the lower incisors. Forty-eight hours after inducing the ulcers, the animals were divided into three groups: laser, LED, and untreated. Animals from the laser group received irradiation with GaAlAs, 660 nm, CW, 40 mW, φ 4 mm(2), 4.8 J/cm(2). Animals from the LED group received irradiation with InGaAIP, 630 nm, 150 mW, 4.8 J/cm(2), 0.8 cm spot. Forty-eight hours after oral ulcer induction, both irradiations were applied in a punctuate manner in the center of the ulcer at 48-h interval until the end of the experimental period. The animals were killed at 3, 5, 7, and 11 days after day 0. The results of the clinical evaluation showed that the laser and LED phototherapies were able to accelerate the healing of formocresol-induced oral ulcers, which occurred first in the laser group (ANOVA, p < 0.05). Histologically, there was a slight variation between LED and laser therapy; therefore, the laser group proved to be effective in accelerating wound healing, especially at 5 days, whereas the LED group was more effective at the end of the experimental period. It was concluded that laser and LED photobiomodulation were effective in accelerating the healing of formocresol-induced oral ulcers in both clinical and histological aspects.

630 nm Light Emitting Diode Irradiation Improves Dermal Wound Healing in Rats

“…Several studies have been reported that 630 nm and 640 nm LED irradia-tion with 4-24 J/cm 2 decreased wound surface area. 10,[15][16][17] Our results agree with the findings from several other studies that used 640 nm and 700 nm LED with 15-16 J/cm 2 to stimulate fibroblast proliferation and collagen production on excision wound in rats. 16,18,19 In the present study, epithelial gap of LED group showed signifi-…”

“…healing of excision wound in the rat. The parameters of LED irradiation used in this study are based on previous studies 7,10,11. LED irradiation was increased the wound closure rate and colla-gen density in situ at day 7 post excision wound, This result suggest that LED irradiation stimulate the fibroblast proliferation and collagen deposition in the granulation tissue.…”

The dark art of light measurement: accurate radiometry for low-level light therapy

“… Larkin-Kaiser et al [ 89 ] No info Source, laser; wavelength, 800 and 970 nm; power, 3 W; frequency, –; beam area, – Irradiance, –; time, 4 min; energy, 360 J; radiant exposure, – Applied to skeletal muscle before resistance exercise, NIR light therapy effectively attenuated strength loss. de Carvalho et al [ 90 ] No info Source, GaAlAs (660 nm, laser) and InGaAlP (630 nm, LED); wavelength, 660 and 630 nm; power, 40 mW (laser) and 150 mW (LED); frequency, CW; beam area, 4 mm 2 (laser) and 0.8 cm (LED) spot Irradiance, –; time, –; energy, –; radiant exposure, 4.8 J/cm 2 Laser and LED photobiomodulation were effective in accelerating the healing of formocresol-induced oral ulcers in both clinical and histological aspects. Ban Frangez et al [ 92 ] No info Source, LED; wavelength, 470, 625, 660 and 850 nm; power, –; frequency, kHz range; beam area, – Irradiance, 2.16–8.23 mW/cm 2 ; time, 3 min; energy, –; radiant exposure, – Photobiomodulation using LED improved the sperm motility in asthenozoospermia regardless of wavelength.…”

The Effect of Using Bone Marrow Mesenchymal Stem Cells Versus Platelet Rich Plasma on the Healing of Induced Oral Ulcer in Albino Rats

“…Rats were anaesthetized with an intramuscular injection of ketamine hydrochloride 10% and xylazine hydrochloride 2% (AMOUN, Egypt) at a dosage of 0.06 ml/100 g and 0.03 ml/100 g, respectively. A homogeneous standardized circular oral cheek ulcer was obtained using 5mm biopsy punch (13), followed by perpendicular application of a flexible swab with a cotton tip soaked in formocresol for 60s (14).…”