Effect of laser zona thinning on vitrified–warmed embryo transfer at the cleavage stage: a prospective, randomized study

Valojerdi, Mojtaba Rezazadeh; Eftekhari‐Yazdi, Poopak; Karimian, Leila; Hassani, Fatemeh; Movaghar, Bahar

doi:10.1016/j.rbmo.2009.11.002

Cited by 25 publications

(18 citation statements)

References 78 publications

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“…The duration of embryos in vitrification solution is critically important due to the use of high concentration of CPAs, which is commonly reported within 1 min (Selman et al, 2009;Stanger et al, 2012;Valojerdi et al, 2010;Zhang et al, 2010). However, the equilibration time varied from 5 to 15 min in different reports (Selman et al, 2009;Stanger et al, 2012;Valojerdi et al, 2010;Zhang et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…For example, the prolongation of exposure time may increase toxicity and influence embryonic developmental potential, whereas shortened exposure may be insufficient for the penetration of CPAs into blastomeres. Most publications emphasized the importance of the duration that embryos should be suspended in the vitrification solution, and most recommend a period of 1 min (Selman et al, 2009;Stanger, Wong, Conceicao, & Yovich, 2012;Valojerdi, Eftekhari-Yazdi, Karimian, Hassani, & Movaghar, 2010;Zhang, Chang, Sone, & Silber, 2010). However, the equilibration times of other studies (Selman et al, 2009;Stanger et al, 2012;Valojerdi et al, 2010;Xie, Zou, Shen, Xiong, & Chen, 2010;Zhang et al, 2010) have ranged from 5 to 15 min.…”

Section: Introductionmentioning

confidence: 99%

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Shortened equilibration time can compromise clinical outcomes in human embryo vitrification

Xiong

Liu²,

Gao

et al. 2016

Human Fertility

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Vitrification is an important way to cryopreserve human embryos and the recommended time of embryo exposure to the vitrification solution is 1 min. However, practically speaking, the duration of embryos exposure to equilibration solution can vary from 5 to 15 min. The purpose of this study was to investigate the effect of different equilibration times on the outcomes of frozen-thawed embryo transfer cycles. The data were collected from our medical records from January 2012 to June 2013 and a total of 517 cycles were included. These cycles were divided into four groups according to the equilibration time: (i) 5-6 min; (ii) 7-8 min; (iii) 9-10 min and (iv) 11-12 min. The results show that there were no differences in terms of survival rate and fully intact embryo rate among the four groups. However, lower clinical pregnancy, embryo implantation and live birth rates were observed in the 5-6 min exposure group (54.6%, 31.9% and 48.2%, respectively) compared with the three other groups. The corresponding rates in the 9-10 min group (73.5%, 47.6% and 64.7%) were the highest. This study indicated that different equilibration times influenced the clinical outcomes of human embryo vitrification and vitrification with shortened equilibration time compromised the clinical outcomes. Appropriate prolongation of the equilibrium time would probably improve the clinical outcomes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Shortened equilibration time can compromise clinical outcomes in human embryo vitrification

Xiong

Liu²,

Gao

et al. 2016

Human Fertility

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show abstract

“…In our work, this decrease is typical for non hatched blastocysts, while sin gle perforation of hatching or completely hatched blasto cysts had no appreciably affect. Utilization of assisted hatching including laser action for extracorporeal fertilization remains questionable [1,16,17]. In the presented work, we show experimentally that laser action on the zona pellucida at different embryo development stages did not significantly affect ability of embryos to develop until the blastocyst stage.…”

Section: Resultsmentioning

confidence: 72%

Effect of laser optoperforation of the zona pellucida on mouse embryo development in vitro

Zakharchenko

Zalessky

Osychenko

et al. 2015

Biochemistry Moscow

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The effect of laser optical perforation of the zona pellucida on the viability and development of mouse embryos has been studied. Operations of zona pellucida thinning and single or double perforation were carried out on 2-cell embryo, morula, and blastocyst stages with a laser pulse (wavelength 1.48 µm, pulse duration 2 ms). Embryo development up to the blastocyst stage and hatching efficiency were statistically analyzed. It was found that 2-cell or morula stage embryo zona pellucida thinning or single perforation did not affect development to the blastocyst stage and number of hatched embryos, but it accelerated embryo hatching compared to control groups one day earlier in vitro. Double optoperforation on 2-cell embryo or morula stage did not significantly affect development to the blastocyst stage, but it strongly decreased the number of hatched embryos. Also, zona pellucida perforation at the blastocyst stage had a negative effect: hatching did not occur after this manipulation. Blastocyst cell number calculation after single zona pellucida perforation at 2-cell and morula stages showed that cell number of hatching or hatched blastocysts did not differ from the same control groups. This fact points out that the laser single optoperforation method is a useful and safe experimental tool that allows further manipulations within the zona pellucida.

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“…Besides, very recent studies of a group reported that the procedure does not improve implantation, clinical pregnancy, or live birth rates in women younger than 38 years with embryos having ZP thickness of ≥13 μm [34,35]. Furthermore another very recent study by Valojerdi showed that clinical pregnancy rate was significantly lower with laser assisted zona thinning on vitrified-warmed cleavage stage embryos [36] although it is widely accepted that it gives beneficial results [27].…”

Section: Discussionmentioning

confidence: 99%

Laser assisted zona thinning technique has no beneficial effect on the ART outcomes of two different maternal age groups

Kutlu¹,

Atvar²,

Vanlioglu³

2010

J Assist Reprod Genet

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Purpose Laser assisted zona thinning is a technique used for facilitating embryo's hatching process and is commonly used for the embryos of poor prognosis patients with advanced maternal age, repeated implantation failures, poor embryo quality or thick zona pellucida. The aim of this study was to investigate the effect of zona thinning on both good or poor prognosis patients of two different age groups. Methods We investigated two different age groups (group 1: <35 years and group 2: ≥35 years ) and compared the effect of assisted zona thinning (groups 1A and 2A) versus not-thinned controls (groups 1B and 2B) in both groups.Results The clinical pregnancy rates were 57% and 56% in groups 1A and 1B (p=0.86), 43% and 38% in groups 2A and 2B (p=0.59) respectively. Conclusions Our results suggest that laser assisted zona thinning of day 3 embryos has no beneficial effect on clinical pregnancy and implantation outcomes.

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Effect of laser zona thinning on vitrified–warmed embryo transfer at the cleavage stage: a prospective, randomized study

Cited by 25 publications

References 78 publications

Shortened equilibration time can compromise clinical outcomes in human embryo vitrification

Shortened equilibration time can compromise clinical outcomes in human embryo vitrification

Effect of laser optoperforation of the zona pellucida on mouse embryo development in vitro

Laser assisted zona thinning technique has no beneficial effect on the ART outcomes of two different maternal age groups

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