Effect of Lactococcus lactis UQ2 and its bacteriocin on Listeria monocytogenes biofilms

García-Almendárez, Blanca E.; Cann, Issac K.O.; Martin, Scott E.; Guerrero‐Legarreta, Isabel; Regalado‐González, Carlos

doi:10.1016/j.foodcont.2007.07.015

Cited by 73 publications

(40 citation statements)

References 45 publications

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“…Results showed that Lc. lactis was able to adhere to silicone surface and produce biofilm exhibited a significant reduction of B. cereus adhered cells after 48 h. In fact, our result was corroborated with García-Almendárez et al [19] showed the competitive exclusion of Listeria by Lc. lactis UQ2 and reported that can be due to the ability of strain to form biofilms on stainless steel coupons at 37°C and may enhance the expression of a clumping protein CluA, a significant adhesion factor of Lactococcus [14].…”

Section: Discussionsupporting

confidence: 90%

Impact of Lactococcus lactis spp. lactis Bio Adhesion on Pathogenic Bacillus cereus Biofilm on Silicone Flowing System

2013

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Bacillus cereus is a food pathogen that can attach on most of the surfaces and form biofilms, which facilitate the persistence and resistance toward antimicrobials. The aims of this study were (i) to characterize the structural dynamics of B. cereus sessile growth in two nutritional environments (with or without a nutrient flow), and (ii) to evaluate the impact of bio adhesion of Lactococcus lactis on B. cereus biofilm. Significantly greater biofilm volume and thickness were observed under dynamic conditions than under static conditions after 48 h and B. cereus biofilm was highly organized. The variation of physico-chemical characteristics of silicone by B. cereus bio adhesion favours the adhesion of hydrophilic Lc. lactis on the surface adhered by biofilm. Lc. lactis was able to adhere to silicone surface and produce biofilm obviously exhibited a significant reduction of B. cereus adhered cells up to nine orders of magnitude after 48 h of contact with competitive activity for nutrient and oxygen. This study constitutes a step ahead in developing strategies to prevent microbial colonization of silicone with lactococcal protective biofilm.

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Section: Discussionsupporting

confidence: 90%

Impact of Lactococcus lactis spp. lactis Bio Adhesion on Pathogenic Bacillus cereus Biofilm on Silicone Flowing System

2013

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“…3b). This is in contrast with the reports of Kleerebezem and Quadri (2001), García-Almendárez et al (2008), and Pongtharangkul and Demirci (2006), who found maximum nisin production at the end of log phase, followed by a decrease in nisin production after this time. This could be probably associated to the absence of inducing nisin in their experiments.…”

Section: Influence Of Factors Used On L Lactis Uq2 Growth and Nisin contrasting

confidence: 99%

“…The nisin A producer L. lactis UQ2 is a native strain isolated from fresh Mexican-style cheese and has been previously reported (García-Almendárez et al 2008). It was identified by sequence similarity of the 1,500 nucleotides of 16S rRNA gene (Midilabs, Newark, DE, USA).…”

Section: Bacterial Strainsmentioning

confidence: 99%

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Effect of Sub-inhibitory Amounts of Nisin and Mineral Salts on Nisin Production by Lactococcus lactis UQ2 in Skim Milk

García-Parra

García-Almendárez

Guevara-Olvera

et al. 2009

Food Bioprocess Technol

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“…Lactococcus lactics UQ2 is a native strain isolated from Mexican-style fresh cheese that synthesizes nisin. L. lactics UQ2 has shown antimicrobial activity against innoculated skim milk (García-Parra et al, 2011) and biofilms (García-Almendárez et al, 2008) of pathogen Listeria monoyctogenes.…”

Section: High Pressure Processing and Biopreservationmentioning

confidence: 99%

HIGH PRESSURE PROCESSING (HPP) AND in-situ NISIN BIOSYNTHESIS BY Lactococcus lactis: A HURDLE APPROACH TO IMPROVE Listeria spp. INACTIVATION IN BOVINE MILK

Alcántara¹

2017

Rev.Mex.Ing.Quim.

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Cold pasteurization of raw milk may be achieved by combining high pressure processing (HPP) with other (a)biotic factors.In this study, a combined approach involving HPP and biopreservation by in-situ nisin synthesis was evaluated. UHT and raw bovine milk were inoculated with Listeria innocua and treated with HPP (350-600 MPa), or HPP+nisin synthesized in-situ by Lactococcus lactis Rif L + (LUQ2). HPP treatments of commercial UHT milk yielded two decimal reductions (S V HPP = 2 log 10 reductions) of L. innocua with 550 MPa/3min, while the HPP+nisin approach yielded <10 cfu ml −1 under the same pressure-time conditions. Native Listeria monocytogenes was found in raw milk (∼10 2 cfu ml −1 ) and the total Listeria spp. counts increased to ∼10 9 cfu ml −1 with the L. innocua inoculum. HPP milk pasteurization (S V HPP ≥ 5 log 10 , aerobic plate count; <1 cfu ml −1 , Listeria spp.) was viable by using 600 MPa/12 min, whereas HPP+nisin resulted in milk pasteurization at holding times between 6 and 9 min for the same pressure level. Furthermore, LUQ2 synthesized 9.75±0.54 IU ml −1 of nisin, which is below the FAO/WHO limit (500 IU g −1 ) suggesting that the promising HPP+nisin approach could be optimized to pasteurize milk at lower pressure levels and/or shorter pressure holding times.

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Effect of Lactococcus lactis UQ2 and its bacteriocin on Listeria monocytogenes biofilms

Cited by 73 publications

References 45 publications

Impact of Lactococcus lactis spp. lactis Bio Adhesion on Pathogenic Bacillus cereus Biofilm on Silicone Flowing System

Impact of Lactococcus lactis spp. lactis Bio Adhesion on Pathogenic Bacillus cereus Biofilm on Silicone Flowing System

Effect of Sub-inhibitory Amounts of Nisin and Mineral Salts on Nisin Production by Lactococcus lactis UQ2 in Skim Milk

HIGH PRESSURE PROCESSING (HPP) AND in-situ NISIN BIOSYNTHESIS BY Lactococcus lactis: A HURDLE APPROACH TO IMPROVE Listeria spp. INACTIVATION IN BOVINE MILK

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