Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines

Pogačar, Maja Šikić; Langerholc, Tomaž; Mičetić-Turk, Dušanka; Možina, Sonja Smole; Klančnik, Anja

doi:10.1186/s12917-020-2238-5

Cited by 18 publications

(16 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The in vitro co-culture with intestinal cells mimics the interactions between probiotics and pathogens in the host GIT. Šikiæ Pogaèar et al (2020) tested the Lactobacillus spp. strains ( Lactobacillus plantarum PCS20, PCS22, PCS25, PCK9, and Lactobacillus rhamnosus LGG) for their competitive adhesion and infection prevention ability against C. jejuni in chicken B1OXI and pig PSI cl.1 epithelial polarized cells.…”

Section: Probiotics Specific For Campylobacter – Imentioning

confidence: 99%

Current Perspectives and Potential of Probiotics to Limit Foodborne Campylobacter in Poultry

et al. 2020

View full text Add to dashboard Cite

Poultry has been one of the major contributors of Campylobacter related human foodborne illness. Numerous interventions have been applied to limit Campylobacter colonization in poultry at the farm level, but other strategies are under investigation to achieve more efficient control. Probiotics are viable microbial cultures that can establish in the gastrointestinal tract (GIT) of the host animal and elicit health and nutrition benefits. In addition, the early establishment of probiotics in the GIT can serve as a barrier to foodborne pathogen colonization. Thus, probiotics are a potential feed additive for reducing and eliminating the colonization of Campylobacter in the GIT of poultry. Screening probiotic candidates is laborious and time-consuming, requiring several tests and validations both in vitro and in vivo. The selected probiotic candidate should possess the desired physiological characteristics and anti-Campylobacter effects. Probiotics that limit Campylobacter colonization in the GIT rely on different mechanistic strategies such as competitive exclusion, antagonism, and immunomodulation. Although numerous research efforts have been made, the application of Campylobacter limiting probiotics used in poultry remains somewhat elusive. This review summarizes current research progress on identifying and developing probiotics against Campylobacter and presenting possible directions for future research efforts.

show abstract

Section: Probiotics Specific For Campylobacter – Imentioning

confidence: 99%

Current Perspectives and Potential of Probiotics to Limit Foodborne Campylobacter in Poultry

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Similar results have been demonstrated in Campylobacter -challenged broilers in which administration of multispecies DFM (PoultryStar ME), composed of Lactobacillus reuteri , Pediococcus acidilactici , Bifidobacterium animalis , and Enterococcus faecium , could improve the transforming growth factor-β4 mRNA content at 14 d post-infection ( Mortada et al., 2020 ). Recent evidence indicates that DFM may enhance host defenses against infection because of the bacteria's effect on host immunity and gut integrity under enteric pathogen challenge ( De Oliveira et al., 2019 ; Šikić Pogačar et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Over the last decade, the genus Campylobacter has become the most identified foodborne pathogen in human gastrointestinal infections. Among the species of Campylobacter capable of causing serious diseases in humans, Campylobacter jejuni is the most common cause of foodborne diseases associated with farm animals, especially chickens ( Saint-Cyr et al., 2017 ; Šikić Pogačar et al., 2020 ). C. jejuni colonizes the chicken ceca at high levels following infection, resulting in fecal shedding of the bacterium.…”

Section: Introductionmentioning

confidence: 99%

Effects of a dietary direct-fed microbial and Ferulago angulata extract on growth performance, intestinal microflora, and immune function of broiler chickens infected with Campylobacter jejuni

et al. 2021

View full text Add to dashboard Cite

Colonization of the gastrointestinal tract by potentially pathogenic bacteria and their shedding in animal feces is a fundamental factor for both animal health and human food safety. This study was conducted to evaluate the efficacy of salinomycin ( Sal ), direct-fed microbial ( DFM ), and Ferulago angulata hydroalcoholic extract ( FAE ) against Campylobacter jejuni in broiler chickens in a 6-week pilot-scale study. A total of six hundred and seventy two 1-day-old broiler chickens were equally divided into 6 groups (each consisting of 8 replicates of 14 birds): negative control ( NC ; untreated and uninfected); positive control ( PC ; untreated, infected with C. jejuni ); PC + Sal; PC + DFM; PC + 200 mg/kg of FAE ( FAE200 ); or PC + 400 mg/kg of FAE ( FAE400 ). All these groups (except NC) were challenged with C. jejuni on day 15. The results showed that all experimental treatments improved ( P < 0.05) average daily gain compared with the PC group, and the best value was observed in the NC and FAE400 groups throughout the entire experimental period (day 1–42). The overall feed conversion ratio and mortality rate, as well as the population of C. jejuni (day 24 and 42) and Coliforms (day 42) in the ileum and cecum, were higher ( P < 0.05) in broiler chickens fed with the PC diet than for chickens in the other groups, except those in the FAE200 group. Immune responses revealed that among challenged birds, those that were fed diets DFM and FAE400 had significantly higher IgG (day 24 and 42), IgA (day 24), IL-6 (day 24), and gamma interferon (day 24 and 42) concentrations than the PC group. In conclusion, dietary FAE, especially at a high level of inclusion in broiler diet (400 mg/kg), could beneficially influence the immune status, as well as improve growth performance and intestinal microflora under Campylobacter challenge, which was comparable to those of Sal and DFM supplements.

show abstract

“…Beside microbiota transplantation, individual or groups of probiotics have been studied to reduce enteric pathogens, such as Lactobacillus helveticus [129], Lactobacillus rhamnosus LGG [130], Lactobacillus gasseri SBT2055 [131], Lactobacillus strains N8, N9, ZL4 and ZL5 [132], Bifidobacterium longum infantis [133]. Lactobacillus enhances macrophage elimination of C. jejuni in vitro and increases the expression of Il1β, Il12p40, Il10, and Cxcl2 and the co-stimulatory molecules CD40, CD80, and CD86 [134].…”

Section: Immunity Microbiota and C Jejuni Interactionmentioning

confidence: 99%

The Role of Immune Response and Microbiota on Campylobacteriosis

Fu¹,

Alenezi²,

Almansour³

et al. 2022

Campylobacter

View full text Add to dashboard Cite

Million cases of campylobacteriosis and complications of post-Campylobacter jejuni infection occur every year around the world with huge life losses and economic burdens of billions of dollars. Few therapy options, such as antibiotics, are available to relieve severe cases of the enteritis. The slow progression on new intervention discovery and application is partially resulted from limited mechanistic understanding on campylobacteriosis pathogenesis. As a type of intestinal disorders, campylobacteriosis shares many common features with other intestinal diseases such as inflammatory bowel diseases (IBD) and Clostridium difficile infection. In pace with the advancement of the gastroenterology field, a large body of knowledge is accumulating on the factors influencing campylobacteriosis onset, development, and outcomes, including host immune response, intestinal microbiota, and its metabolites. In this chapter, we review the intestinal immune system, intestinal microbiome, and microbiome modulation of inflammation in the development of campylobacteriosis. The interplay between immunity, microbiota, and its metabolites may play essential roles on campylobacteriosis pathogenesis and the finding on the interaction may lead to new prevention and treatment options. The purpose of this chapter is to provide updated knowledge on the role of host–microbe interaction and the therapeutic potential on campylobacteriosis.

show abstract

Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines

Cited by 18 publications

References 58 publications

Current Perspectives and Potential of Probiotics to Limit Foodborne Campylobacter in Poultry

Current Perspectives and Potential of Probiotics to Limit Foodborne Campylobacter in Poultry

Effects of a dietary direct-fed microbial and Ferulago angulata extract on growth performance, intestinal microflora, and immune function of broiler chickens infected with Campylobacter jejuni

The Role of Immune Response and Microbiota on Campylobacteriosis

Contact Info

Product

Resources

About