Effect of Lactobacillus rhamnosus on the response of Galleria mellonella against Staphylococcus aureus and Escherichia coli infections

Jorjão, Adeline Lacerda; Oliveira, Felipe Eduardo de; Leão, Mariella Vieira Pereira; Jorge, Antônio Olavo Cardoso; Oliveira, Luciane Dias de

doi:10.1007/s00203-017-1441-7

Cited by 17 publications

(10 citation statements)

References 19 publications

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“…One investigation showed that a HIL extract prevented growth of plant pathogens and suggested its use as a natural antimicrobial [ 28 ]. Furthermore, Lactobacillus species activated innate immune response in insects and contributed to reduction of susceptibility in pathogen infected-insects [ 29 , 30 , 31 , 32 ]. In this investigation, we also found HIL extract immunized with L. casei exhibited antimicrobial activity against three Salmonella species ( Figure 4 B), which demonstrates that this extract offers a potential means of preventing and treating plant pathogen infections and protecting against Salmonella contamination.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity of an Extract of Hermetia illucens Larvae Immunized with Lactobacillus casei against Salmonella Species

Lee

Yun

Goo

2020

Insects

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The expressions of antimicrobial peptides (AMPs) in the larvae of the black soldier fly, Hermetia illucens, were significantly increased by pathogen or stimulant induced innate immunity activation. We immunized H. illucens fifth instar larvae with five different Lactobacillus species, that is, Lactobacillus acidophilus, L. brevis, L. casei, L. fermentum, or L. delbrueckii, to induce the mass production of AMPs and selected optimal immune inducers. Antimicrobial activities in hemolymph and H. illucens larvae (HIL) extract were evaluated against three salmonella species (Salmonella pullorum, Salmonella typhimurium, and Salmonella enteritidis). Highest antimicrobial activity was shown by the hemolymph of HIL immunized by L. casei and its activity was closely linked with the inductions of cecropin 1 (HiCec1) and defensin 1 (HiDef1) gene expressions. Furthermore, antimicrobial activity in hemolymph was stable to heat and pH and the growth of three Salmonella species were dramatically suppressed by HIL hemolymph and extract after immunization with L. casei. The minimal inhibitory concentration (MICs) of L. casei-immunized HIL extract against Staphylococcus aureus, Escherichia coli, and Salmonella species ranged from 100~200 µg/100 µL and no cytotoxicity to CaCo-2 and L929 cells were observed in the concentration range 100~40,000 µg/100 µL. Taken together, the present investigation demonstrates that L. casei-immunized HIL extract is a powerful natural antibiotic and preservative that can prevent contamination by Salmonella species.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity of an Extract of Hermetia illucens Larvae Immunized with Lactobacillus casei against Salmonella Species

Lee

Yun

Goo

2020

Insects

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“…The melanization pattern of G. mellonella larvae may also be affected by use of conventional antimicrobials. Some studies used larvae to evaluate different therapeutic strategies [146,147,148]. Alcazar-Fuoli et al [147] evaluated the effectiveness of voriconazole in G. mellonella infected by Aspergillus fumigatus .…”

Section: Humoral Immune Responsementioning

confidence: 99%

“…Bacterial infections also cause the melanization of G. mellonella larvae. Recently, Jorjão et al [146] studied the melanization of G. mellonella against Staphylococcus aureus and Escherichia coli infections. These authors verified an increased melanization of larvae after 24 h of the inoculation with S. aureus or E. coli proving that these bacteria are able to activate the phenoloxidase enzyme.…”

Section: Humoral Immune Responsementioning

confidence: 99%

Recent Advances in the Use of Galleria mellonella Model to Study Immune Responses against Human Pathogens

Pereira

Barros

Fugisaki

et al. 2018

JoF

122

108

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The use of invertebrates for in vivo studies in microbiology is well established in the scientific community. Larvae of Galleria mellonella are a widely used model for studying pathogenesis, the efficacy of new antimicrobial compounds, and immune responses. The immune system of G. mellonella larvae is structurally and functionally similar to the innate immune response of mammals, which makes this model suitable for such studies. In this review, cellular responses (hemocytes activity: phagocytosis, nodulation, and encapsulation) and humoral responses (reactions or soluble molecules released in the hemolymph as antimicrobial peptides, melanization, clotting, free radical production, and primary immunization) are discussed, highlighting the use of G. mellonella as a model of immune response to different human pathogenic microorganisms.

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“…Tests were performed in biological and experimental triplicate, with 10 larvae per replicate. Melanin production was quantified as previously described ( Jorjão et al, 2018 ), with modifications. Briefly, incisions were made in the larvae’s prolegs with a micro scissor to allow the hemolymph to leak.…”

Section: Methodsmentioning

confidence: 99%

Identification of small RNAs associated with RNA chaperone Hfq reveals a new stress response regulator in Actinobacillus pleuropneumoniae

et al. 2022

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The RNA chaperone Hfq promotes the association of small RNAs (sRNAs) with cognate mRNAs, controlling the expression of bacterial phenotype. Actinobacillus pleuropneumoniae hfq mutants strains are attenuated for virulence in pigs, impaired in the ability to form biofilms, and more susceptible to stress, but knowledge of the extent of sRNA involvement is limited. Here, using A. pleuropneumoniae strain MIDG2331 (serovar 8), 14 sRNAs were identified by co-immunoprecipitation with Hfq and the expression of eight, identified as trans-acting sRNAs, were confirmed by Northern blotting. We focused on one of these sRNAs, named Rna01, containing a putative promoter for RpoE (stress regulon) recognition. Knockout mutants of rna01 and a double knockout mutant of rna01 and hfq, both had decreased biofilm formation and hemolytic activity, attenuation for virulence in Galleria mellonella, altered stress susceptibility, and an altered outer membrane protein profile. Rna01 affected extracellular vesicle production, size and toxicity in G. mellonella. qRT-PCR analysis of rna01 and putative cognate mRNA targets indicated that Rna01 is associated with the extracytoplasmic stress response. This work increases our understanding of the multilayered and complex nature of the influence of Hfq-dependent sRNAs on the physiology and virulence of A. pleuropneumoniae.

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Effect of Lactobacillus rhamnosus on the response of Galleria mellonella against Staphylococcus aureus and Escherichia coli infections

Cited by 17 publications

References 19 publications

Antimicrobial Activity of an Extract of Hermetia illucens Larvae Immunized with Lactobacillus casei against Salmonella Species

Antimicrobial Activity of an Extract of Hermetia illucens Larvae Immunized with Lactobacillus casei against Salmonella Species

Recent Advances in the Use of Galleria mellonella Model to Study Immune Responses against Human Pathogens

Identification of small RNAs associated with RNA chaperone Hfq reveals a new stress response regulator in Actinobacillus pleuropneumoniae

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