Effect of L-lysine in culture medium on nodule formation by bone marrow cells

Abstract

The aim of this study was to estimate the effect of L-lysine on nodule formation by rat bone marrow cells <i>in vitro</i>. In this study, L-lysine was added to medium for mesenchymal stem cell culture to promote proliferation and differentiation of the cells, and then nodule formation was estimated in an <i>in vitro</i> rat bone marrow cell culture. Bone marrow cells from the bone shafts of the femora of Fischer 344 rats were cultured in minimum essential medium with 20 μl of L-lysine solution at 10<sup>﹣4</sup>, 10<sup>﹣5</sup>, 10<sup>﹣6</sup>, 10<sup>﹣7</sup> or 10<sup>﹣8</sup> M. Dexamethasone was also added to the medium at 10 nM for differentiation of stem cells from bone marrow into osteoblast progenitor cells. The subculture was performed for 2 weeks. The quantity of osteocalcin in rat bone marrow cell culture with dexamethasone was 392 ng/ml. In the medium with dexamethasone and 10<sup>﹣8</sup> M L-lysine, the quantity of osteocalcin was 437 ng/ml. Nodules only formed upon addition of 20 μl of L-lysine at 10<sup>﹣8</sup> M. It was indicated that 10<sup>﹣8</sup> M L-lysine should be the optimal concentration for calcification. For nodule formation by rat bone marrow cells <i>in vitro</i>, the optimum concentration of L-lysine in culture medium might be 20 μl of 10<sup>﹣8</sup> M. L-lysine could play an important role in matrix production for bone formation <i>in vitro</i>

Influence of Hydroxyapatite Surface Functionalization on Thermal and Biological Properties of Poly(l-Lactide)- and Poly(l-Lactide-co-Glycolide)-Based Composites

“…In place of such commonly used reagents, l- lysine was proposed to assist PEG grafting and to improve osteological bioactivity of the composites. l- lysine is known to promote the adhesion of osteoblasts to the surface of the material, which may positively correlate with cell proliferation [ 10 , 11 ], and was found to improve the osteogenic potential of the bone marrow stromal cells [ 12 ]. l- lysine can be used as a food supplement and its intake benefits in increased bone mineral density and lowered prevalence of osteoporosis [ 13 ].…”

<i>In Vivo</i> Estimation of Osteogenesis by Bone Marrow Cells in a Bi-Phasic Scaffold and in Each of Its Components

“…It was also reported that nutrition for the rBMCs in the pores may be supplied through the hollow center of the scaffold. Furthermore, adhesion of the rBMCs to the pore wall of the scaffold can be effectively encouraged by coating the scaffold with chemical agents [10] [11].…”

Bone Formation in a Scaffold Composed of Cylindrical Hydroxyapatite and Tryptophan- or Lysine-Coated Sponge <i>in Vivo</i>

“…The selection of an effective supplement is desirable for the promotion of stem cell proliferation in culture. Yoshikawa et al reported that some amino acids had the possibility of promoting bone formation by bone marrow cells in a porous HA scaffold [21]. Thus, amino acids were evaluated in terms of their ability to promote bone formation using rBMCs as a supplement by coating on the materials in the scaffold in this study.…”