Effect of L-carnitine on the expression of the apoptotic genes Bcl-2 and Bax

Vardiyan, Reyhane; Ezati, Daniyal; Anvari, Morteza; Ghasemi, Nasrin; Talebi, Alireza

doi:10.5653/cerm.2019.03440

Cited by 19 publications

(15 citation statements)

References 40 publications

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“…In a study involving rats, L-carnitine taken before doxorubicin, a chemotherapy drug, partially preserved the acrosome integrity of sperm [45]. In another study on mice, L-carnitine raises Bcl-2 levels and reduces Bax expression, indicating that this compound may inhibit apoptosis [46]. Several studies on L-carnitine involved also antimicrobials, anti-inflammatory drugs or pentoxifylline [47][48][49][50].…”

Section: L-carnitinementioning

confidence: 99%

Oxidative Stress and Male Fertility: Role of Antioxidants and Inositols

et al. 2021

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Infertility is defined as a couple’s inability to conceive after at least one year of regular unprotected intercourse. This condition has become a global health problem affecting approximately 187 million couples worldwide and about half of the cases are attributable to male factors. Oxidative stress is a common reason for several conditions associated with male infertility. High levels of reactive oxygen species (ROS) impair sperm quality by decreasing motility and increasing the oxidation of DNA, of protein and of lipids. Multi-antioxidant supplementation is considered effective for male fertility parameters due to the synergistic effects of antioxidants. Most of them act by decreasing ROS concentration, thus improving sperm quality. In addition, other natural molecules, myo-inositol (MI) and d-chiro–inositol (DCI), ameliorate sperm quality. In sperm cells, MI is involved in many transduction mechanisms that regulate cytoplasmic calcium levels, capacitation and mitochondrial function. On the other hand, DCI is involved in the downregulation of steroidogenic enzyme aromatase, which produces testosterone. In this review, we analyze the processes involving oxidative stress in male fertility and the mechanisms of action of different molecules.

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Section: L-carnitinementioning

confidence: 99%

Oxidative Stress and Male Fertility: Role of Antioxidants and Inositols

et al. 2021

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“…BCL‐2 protein, as a proto‐oncogene in germ cells, is involved in the regulation of cell apoptosis. 23 Thus, this protein is generally involved in inhibiting cellular apoptosis. If BCL ‐ 2 gene expression is reduced, the process of programmed cell death or apoptosis begins with activation of other oncogenes such as P53 .…”

Section: Discussionmentioning

confidence: 99%

“…BCL‐2 protein is located in the mitochondrial membrane and endoplasmic reticulum. BCL‐2 protein, as a proto‐oncogene in germ cells, is involved in the regulation of cell apoptosis 23 . Thus, this protein is generally involved in inhibiting cellular apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Royal‐jelly‐based apitherapy can attenuate damages to male reproductive parameter following nicotine administration

Nazar-Zadeh

Jalili

Fathi

et al. 2022

Anim Models and Exp Med

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Background Nicotine administration can generate severe oxidative stress and lipid peroxidation. Royal jelly, with its antioxidant properties, acts as a scavenger of reactive oxygen species. This study describes the apitherapy effects of royal jelly on testicular damage following nicotine administration. Methods Forty‐eight male BALB/c mice were divided into 8 groups ( n = 6): saline, 3 different doses of royal jelly (100, 150, and 200 mg/kg body weight (BW) per day), nicotine (1.5 mg/kg), and 3 different groups of Nic + Roy (1.5 mg/kg of Nic + 100, 150, and 200 mg/kg BW per day of royal jelly). Nicotine was administrated intraperitoneally, and royal jelly was prescribed orally for 10 consecutive days. Serum levels of hormones (testosterone, luteinizing hormone, and follicle‐stimulating hormone), total antioxidant capacity, nitric oxide (NO) status, malondialdehyde levels, sperm DNA fragmentation, sperm parameters, histopathological changes (H&E staining), immunohistochemistry against apoptotic proteins, and gene expression of Bcl ‐ 2 , p53 , Caspase ‐ 3 , and Nrf2 (real‐time PCR) were assessed to evaluate the molecular and histological changes. Results Hormone levels, sperm parameters, and status of antioxidants were decreased significantly ( p < .05) following nicotine administration. Moreover, royal jelly treatment normalized hormonal and antioxidant characteristics, decreased apoptotic gene expression, increased Nfr2 gene expression, and restored histopathological alteration to the physiological status significantly ( p < .05). Conclusion Royal jelly upregulates the antioxidant status, inhibits the mitochondrial‐dependent apoptosis pathway, and increases the rate of proliferation. This therapeutic agent effectively protected the testis against nicotine‐associated damages by antioxidant and anti‐apoptotic effects.

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“…Apoptosis has been documented as dispensation over several machineries, including amendment of the intracellular mitochondrial depended pathway to stimulate the caspase cascade activation [44]. When the over production of ROS sprightly induces proaptotic mediators leads to apoptosis [45]. ZnO NPs treatment arbitrated apoptotic protein appearance was studied using western blotting analysis.…”

Section: Znonp-gs Induces the Production Of Ros And Apoptosis In Mcf-7 Cellsmentioning

confidence: 99%

Biological Fabrication and Characterization of Zinc Oxide Nanoparticles Using Gymnema Sylvestre Potentially Produces Toxicity in Breast Cancer Cells -MCF-7

Santhoshkumar

Balupillai

Ernest

2021

Preprint

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To study the biological fabrication and characterization of zinc oxide nanoparticles (ZnO NPs) using Gymnema sylvestre and their toxicity to breast cancer cells MCF-7.In the existing work, ZnO NPs were synthesized using leaf extract of the Indian medicinal plant Gymnema sylvestre and it was characterized by Particle size, Zeta potential, FT-IR, XRD and SEM analyses.This ZnO NPs to have potentially validated anticancer role in breast cancer cells MCF-7 in vitro approach.The plant-based synthesized ZnO NPs were evaluated against the inhibitory role on breast cancer cell lines. We significantly observed that ZnO-NPs induce efficient toxicity of MCF-7 cells by increasing ROS, mitochondrion membrane damage and apoptotic morphological alterations. These results stated that ZnO-NP induces Bax and Caspases and down-regulates Bcl-2 proteins in MCF-7 cells. Thus, the biologically synthesized ZnO NPs were identified as good performance to inhibit breast cancer cell growth even at low concentrations.

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Effect of L-carnitine on the expression of the apoptotic genes Bcl-2 and Bax

Cited by 19 publications

References 40 publications

Oxidative Stress and Male Fertility: Role of Antioxidants and Inositols

Oxidative Stress and Male Fertility: Role of Antioxidants and Inositols

Royal‐jelly‐based apitherapy can attenuate damages to male reproductive parameter following nicotine administration

Biological Fabrication and Characterization of Zinc Oxide Nanoparticles Using Gymnema Sylvestre Potentially Produces Toxicity in Breast Cancer Cells -MCF-7

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