The aim of this research was to examine the impacts of lines, L-carnitine, and their combination on laying performance and characteristics of egg quality. A total of 480 newly hatched birds in three lines (desert, brown and white) of Kurdish quail with four levels of L-carnitine (0, 150, 350 and 550 mg/kg)were randomly distributed into four treatments, with each treatment consisting of four replicates, each containing 10 quails and birds were raised from 1 to 12 weeks of age.The results showed that adding different levels of L-carnitine had a higher (p ≤ 0.05) impact on each quantity of eggs, daily egg production (HD%), and egg weight compared to the control group and affected some egg quality parameters. Furthermore, supplementation with 350 and 550 mg/kg L-carnitine throughout the experimental period significantly improved these characteristics compared with the other experimental treatments having the highest values. As well as for the interacted groups, the white lines with the diet of 350 mg/kg L-carnitine had a significant influence on (HD%), egg weight during 7 -12 weeks of age, and influenced several egg quality characteristics. Except for The addition of L-carnitine to the diet had no significant effect on shape index, yolk height, yolk percentage, shell weight, or shell percentage features. Based on the present results, it can be stated that supplementing the diet with L-carnitine at levels of 350 and 550 mg/kg improves laying performance and some exterior and interior egg quality in three lines of Kurdish quail.