Effect of kitasamycin and nitrofurantoin at subinhibitory concentrations on quorum sensing regulated traits of Chromobacterium violaceum

Ibrahim, Yasser Musa; Abouwarda, Ahmed Megahed; Omar, Farghaly A.

doi:10.1007/s10482-020-01467-6

Cited by 8 publications

(9 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…45 This type of antibiotic was extensively used in agriculture as growth-promoting additive for swine and poultry. 46,47 Thus, antibiotic resistance in association with Kit resistance was reported in Brachyspira hyodysenteriae, 48 Chromobacterium violaceum, 33 and E. coli. 46 The resistant mechanism involved the resistant gene mccE that acetylated Kit and converted it into a nontoxic compound 49 or mutation on FhuD and FhuB, the target of Kit.…”

Section: ■ Discussionmentioning

confidence: 99%

“…Kit belongs to macrolide antibiotic that is produced by the species of Streptomyces kitasatoensis. 33 Kit was often used to treat bacterial infections because of its broad bactericidal ability, such as Grampositive cocci infections, including Streptococcus pyogenes and Staphylococcus aureus. 45 This type of antibiotic was extensively used in agriculture as growth-promoting additive for swine and poultry.…”

Section: ■ Discussionmentioning

confidence: 99%

“…Meanwhile, we also found that the expression of ETAE_0615 (the periplasmic component trivalent iron ions hydroxamate transport system with ABC-type) was increased. Although ETAE_0615 is transporting the ferrichrome-iron across the membrane, it is also the transporter for antibiotic Kit, 33 raising the possibility that LTB4-R may elevate the uptake of Kit. Thus, PPI analysis demonstrates that bacteria enhance siderophore biosynthesis and Fe 3+ -hydroxamate transport upon NOR resistance.…”

Section: Mapping Of Protein−protein Interaction Network Of Depsmentioning

confidence: 99%

See 2 more Smart Citations

Functional Proteomics Analysis of Norfloxacin-Resistant Edwardsiella tarda

Wu,

Li,

Tan

et al. 2023

J. Proteome Res.

View full text Add to dashboard Cite

Multidrug-resistant Edwardsiella tarda threatens both sustainable aquaculture and human health, but the control measure is still lacking. In this study, we adopted functional proteomics to investigate the molecular mechanism underlying norfloxacin (NOR) resistance in E. tarda. We found that E. tarda had a global proteomic shift upon acquisition of NOR resistance, featured with increased expression of siderophore biosynthesis and Fe3+-hydroxamate transport. Thus, either inhibition of siderophore biosynthesis with salicyl-AMS or treatment with another antibiotic, kitasamycin (Kit), which was uptake through Fe3+-hydroxamate transport, enhanced NOR killing of NOR-resistant E. tarda both in vivo and in vitro. Moreover, the combination of NOR, salicyl-AMS, and Kit had the highest efficacy in promoting the killing effects of NOR than any drug alone. Such synergistic effect not only confirmed in vitro and in vivo bacterial killing assays but also applicable to other clinic E. tarda isolates. Thus, our data suggest a proteomic-based approach to identify potential targets to enhance antibiotic killing and propose an alternative way to control infection of multidrug-resistant E. tarda.

show abstract

Section: ■ Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

Section: Mapping Of Protein−protein Interaction Network Of Depsmentioning

confidence: 99%

See 1 more Smart Citation

Functional Proteomics Analysis of Norfloxacin-Resistant Edwardsiella tarda

Wu,

Li,

Tan

et al. 2023

J. Proteome Res.

View full text Add to dashboard Cite

show abstract

“…Furthermore, Xu et al (2017) reported that the expression of vio B , vioC , and v ioD enzymes was more important than other enzymes ( vioA and vioE ) during violacein biosynthesis by using a statistical approach for metabolic engineering [ 26 ]. Subsequently, Musa Ibrahim et al (2020) determined that kitasamycin and nitrofurantoin downregulated the vioB , vioC , and v ioD enzymes [ 27 ]. Our qRT–PCR results presented here are consistent with previous reports, which studied the effect of QSIs from plant sources on the expression of QS-related genes [ 28 , 29 ].…”

Section: Discussionmentioning

confidence: 99%

The Derived Components of Gnaphalium hypoleucum DC. Reduce Quorum Sensing of Chromobacterium violaceum

Chu

Liu

et al. 2022

Molecules

View full text Add to dashboard Cite

Gnaphalium hypoleucum DC. was first recorded in the Chinese National Pharmacopoeia “Yi Plant Medicine”. There is no detailed report on its main components’ activity in suppressing the quorum sensing activity (QS) of bacteria. Our study aimed to screen the main components in extracts of G. hypoleucum DC. in order to measure their effects on bacterial QS activity and to explore specific quorum sensing mechanisms that are affected by G. hypoleucum DC. extracts. Crude extracts of G. hypoleucum DC. contained significant amounts of two compounds shown to inhibit bacterial QS activity, namely apigenin and luteolin. Apigenin and luteolin in crude extracts of G. hypoleucum DC. showed substantial inhibition of pigment formation, biofilm production, and motility in Chromobacterium violaceum ATCC 12472 compared to the effects of other phytochemicals from G. hypoleucum DC. Apigenin and luteolin exhibited a strong QS inhibitory effect on C. violaceum, interfering with the violacein pigment biosynthesis by downregulating the vioB, vioC, and vioD genes. In the presence of signal molecules, the QS effect is prevented, and the selected compounds can still inhibit the production of the characteristic purple pigment in C. violaceum. Based on qualitative and quantitative research using genomics and bioinformatics, we concluded that apigenin and luteolin in crude extracts of G. hypoleucum DC can interfere with the generation of QS in C. violaceum by downregulating the vioB, vioC, and vioD genes. Indeed, G. hypoleucum DC. is used for the treatment of bacterial infections, and this research provides new ideas and potential alternative uses for medicinal plants.

show abstract

“…Vancomycin MICs were graded to evaluate the strain susceptibility using the broth microdilution methods [ 26 ]. Briefly, ten serial dilutions of vancomycin (Sigma-Aldrich), were prepared at concentrations of 32 to 0.06 μg/mL and inoculated with standardized bacterial suspension of 0.5McFarlan turbidity.…”

Section: Methodsmentioning

confidence: 99%

Emerging vancomycin-non susceptible coagulase negative Staphylococci associated with skin and soft tissue infections

Akinduti

Obafemi

Ugboko

et al. 2022

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

Backgrounds Observable emergence of Vancomycin-Non susceptible Coagulase-negative Staphylococci (VNS-CoNS) associated with skin and soft tissue infections spreading among the urban and rural populace is gradually intensifying severe complications. The isolated VNS-CoNS were evaluated with Matrix-assisted Laser Desorption/ionization Time of Flight Mass Spectrometry (MALDI ToF MS) for species characterization and pan-antimicrobial resistance pattern. Methods Out of 256 clinical samples collected including pus, abscess, ear swabs, eye swabs, and aspirates, 91 CoNS isolates were biotyped and further characterized with MALDI-TOF MS. Staphylococci marker genes, Vancomycin susceptibility, and biofilm assays were performed. Results Of 91 CoNS isolates, S.cohnii (2.3%), S.condimentii (3.4%), S. saprophyticus (6.7%), and S.scuri (21.1%) were characterized with MALDI-TOF with significant detection rate (99.4%; CI 95, 0.775–0.997, positive predictive values, 90.2%) compared to lower biotyping detection rate (p = 0.001). Hemolytic VNS-CoNS lacked nuc, pvl and spa genes from wound, ear, and aspirates of more 0.83 MARI clustered into a separate phylo-diverse group and were widely distributed in urban and peri-urban locations. MALDI TOF–MS yielded a high discriminatory potential of AUC-ROC score of 0.963 with true-positivity prediction. VNS-CoNS of MIC ≥ 16 µg/mL were observed among all the ages with significant resistance at 25th and 75th quartiles. More than 10.5% of CoNS expressed multi-antibiotic resistance with more than 8 µg/mL vancomycin cut-off values (p < 0.05). Conclusion Antibiotic resistant CoNS should be considered significant pathogens rather than contaminant. Biofilm producing VNS-S. sciuri and S. condimentii are potential strains with high pathological tropism for skin, soft tissues and wound infections, and these strains require urgent surveillance in peri-urban and rural communities.

show abstract

Effect of kitasamycin and nitrofurantoin at subinhibitory concentrations on quorum sensing regulated traits of Chromobacterium violaceum

Cited by 8 publications

References 50 publications

Functional Proteomics Analysis of Norfloxacin-Resistant Edwardsiella tarda

Functional Proteomics Analysis of Norfloxacin-Resistant Edwardsiella tarda

The Derived Components of Gnaphalium hypoleucum DC. Reduce Quorum Sensing of Chromobacterium violaceum

Emerging vancomycin-non susceptible coagulase negative Staphylococci associated with skin and soft tissue infections

Contact Info

Product

Resources

About