Effect of Kelulut Honey on the Cellular Dynamics of TGFβ-Induced Epithelial to Mesenchymal Transition in Primary Human Keratinocytes

Nordin, Abid; Chowdhury, Shiplu Roy; Saim, Aminuddin Bin; Idrus, Ruszymah Bt Hj

doi:10.3390/ijerph17093229

Cited by 10 publications

(6 citation statements)

References 34 publications

(42 reference statements)

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“…Keloid scar formation is due to the excessive induction of EMT by TGFβ in keratinocytes. In a study, treatment of 0.0015% Kelulut honey has been demonstrated to reduce the TGFβ-induced EMT in human primary keratinocytes, indicating Kelulut honey’s therapeutic potential in preventing keloid scar formation [ 132 ]. Rats pretreated with Kelulut honey showed significantly reduced total area of ulcer and ulcer index compared with an untreated ethanol-induced gastric ulcer rat [ 133 ].…”

Section: Medicinal Properties Of Tualang Gelam and Kelulut Honeysmentioning

confidence: 99%

Physicochemical and Medicinal Properties of Tualang, Gelam and Kelulut Honeys: A Comprehensive Review

et al. 2021

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Tualang, Gelam and Kelulut honeys are tropical rainforest honeys reported to have various medicinal properties. Studies related to the medicinal properties and physicochemical characteristics of these honeys are growing extensively and receiving increased attention. This review incorporated and analysed the findings on the biological and physicochemical properties of these honeys. Tualang, Gelam and Kelulut honeys were found to possess a wide variety of biological effects attributed to their physicochemical characteristics. Findings revealed that these honeys have anti-diabetic, anti-obesity, anti-cancer, anti-oxidative, anti-microbial, anti-inflammatory and wound-healing properties and effects on the cardiovascular system, nervous system and reproductive system. The physicochemical properties of these honeys were compared and discussed and results showed that they have high-quality contents and excellent antioxidant sources.

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Section: Medicinal Properties Of Tualang Gelam and Kelulut Honeysmentioning

confidence: 99%

Physicochemical and Medicinal Properties of Tualang, Gelam and Kelulut Honeys: A Comprehensive Review

et al. 2021

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“…However, in recent years, more and more studies have revealed that epidermal keratinocytes also play an important role in wound healing and scar formation [2,3]. During scar formation, keratinocytes gradually lose their characteristics of epithelial cells and acquire features of mesenchymal cells, manifested by decreased ability of cell adhesion and increased ability of migration [4]. However, it is still largely unknown that AGING how is the epithelial-mesenchymal transition (EMT) process regulated in dermal keratinocytes.…”

Section: Introductionmentioning

confidence: 99%

S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn

Xu¹,

Cheng²,

Kong³

et al. 2021

aging

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S100 calcium-binding protein A8 (S100A8) and S100A9 are small molecular weight calcium-binding regulatory proteins that have been involved in multiple chronic inflammatory diseases. However, the role of S100A8 and S100A9 in keratinocytes in wounded skin and how they are regulated during this process are still unclear. Here, we found that S100A8 and S100A9 were both upregulated in burn-wounded skins in vivo and thermalstimulated epidermal keratinocytes in vitro, accompanied by increased levels of epithelial-mesenchymal transition (EMT). Then, we demonstrated that upregulation of S100A8 and S100A9 alone or together enhanced characteristics of EMT in normal keratinocytes, manifested by excessive proliferation rate, abnormal ability of cell invasion, and high expression levels of EMT marker proteins. The transcription factor PU box-binding protein (PU.1) bound to the promoter regions and transcriptionally promoted the expression of S100A8 and S100A9 both in the human and mice, and it had strong positive correlations with both S100A8 and S100A9 protein levels in burned skin in vivo. Moreover, PU.1 positively regulated expression of S100A8 and S100A9 in a dose-dependent manner, and enhanced EMT of keratinocytes in vitro. Finally, through the burn mouse model, we found that PU.1 -/mice displayed a lower ability of scar formation, manifested by smaller scar volume, thickness, and collagen content, which could be enhanced by S100A8 and S100A9. In conclusion, PU.1 transcriptionally promotes expression of S100A8 and S100A9, thus positively regulating epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn.

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“…Davison SP indicated that epidermal‐mesenchymal interaction might play a key role in the pathogenesis of keloid, and further suggested combination therapy appears to be the most efficacious and safest keloid management option available 31 . It is the latest research trend to apply epithelial‐to‐mesenchymal transition to coordinate the complex pathway mechanism of keloid and to view the occurrence and development of keloid from a dynamic perspective 32,33 . TGF‐β is the most critical growth factor in keloid formation.…”

Section: Discussionmentioning

confidence: 99%

Global research status of pathological scar reported over the period 2001–2021: A 20‐year bibliometric analysis

Ding

Yang

et al. 2022

International Wound Journal

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Pathological scar is a classic problem in plastic and reconstructive surgery. Although the researches on pathological scar have been conducted for decades, the way to go to address this thorny problem still remains challenging. To the best of our knowledge, few bibliometric analysis concerning pathological scar have been reported. In this study, we set out to employ bibliometric and visual analysis to offer research status and trends of pathological scar over the period 2001–2021. All publications covering pathological scar during 2001–2021 were retrieved and extracted from the Web of Science database. We applied VOSviewer software to evaluate the keywords and research hotpots, and the online tool (http://bibliometric.com/) was used to carried out the publication trends analysis. A total of 2221 pathological scar‐related articles were identified over the period 2001–2021. China is the country which had the largest volume of publications (819, 36.87%), followed by the United States (416, 18.73%), Japan (144, 6.48%), Korea (142, 6.39%), and England (118, 5.31%). Among the institutions and journals, Shanghai Jiao Tong University (167) and Wound Repair and Regeneration (85) accounted for the most papers related to pathological scar, respectively. Professor Bayat A, who had the most citation frequency (2303), made great contribution in pathological scar field. “Fibroblast”, “expression”, and “proliferation” were identified as the pathological scar research hotspot through analysis of the keywords. In terms of publication, China ranked first all over the world, but the numbers of publication are inconsistent with the citation frequency, ranking first and second, respectively. Shanghai Jiao Tong University and journal Wound Repair and Regeneration stand for the highest level of research in this field to a certain extent. In the early stage, the research focus was mainly on the prevention, treatment, and risk factors for recurrence of pathological scar from cases. In the later stage, the research focus was on the comprehensive management, in which the mechanism research was in‐depth to the molecular and gene level.

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Effect of Kelulut Honey on the Cellular Dynamics of TGFβ-Induced Epithelial to Mesenchymal Transition in Primary Human Keratinocytes

Cited by 10 publications

References 34 publications

Physicochemical and Medicinal Properties of Tualang, Gelam and Kelulut Honeys: A Comprehensive Review

Physicochemical and Medicinal Properties of Tualang, Gelam and Kelulut Honeys: A Comprehensive Review

S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn

Global research status of pathological scar reported over the period 2001–2021: A 20‐year bibliometric analysis

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