Effect of Javanese Turmeric (Curcuma xanthorrhiza) Extract on Hepatitis Model of Alcohol-Induced Mice

Abstract

<p class="KEYWORDS">Alcoholic hepatitis is a common cause of non-viral hepatitis. Javanese turmeric (Curcuma xanthorrhiza) contains xanthorrhizol and curcuminoid that have antioxidant and anti-inflammatory effects. The purpose of this research was to detect the effect of Curcuma xanthorrhiza in decreasing hydropic degeneration and lymphocytes in alcohol-induced mice. This experimental research used post-test only controlled design. Randomly, 35 mice were divided into five groups. Four groups were induced by alcohol 30% of 4ml/kgBW in 10 days, and one group was normal group (negative control). Three of the four alcohol-induced groups were given Curcuma xanthorrhiza extract at doses of 42, 84, and 168 mg/kgBW in 10 days, 1 hour after alcohol induction. Mice livers were taken for Hematoxylin Eosin (HE) staining. Hydropic degeneration and lymphocytes in the liver lobules were examined under a microscope. The statistical analysis used in this research was the Kruskal-Wallis, the Mann-Whitney test, and the Independent Sample T Test. P-value is significant if <0.05. The result of this research were all doses of Javanese turmeric extract significantly reduced hydropic degeneration. Doses of 84 and 168 mg/kgBW also reduced inflammatory lymphocytes (p<0.05). The dose of 168 mg/kgBW reduced the lymphocyte inflammation maximally. There was no significant difference between increasing Javanese turmeric dose and decreasing hydropic degeneration and lymphocytes (p>0.05). This research shows that Javanese turmeric (Curcuma xanthorrhiza) can attenuate hepatitis by decreasing hydropic degeneration and lymphocytes in alcohol-induced liver.</p>

Immunomodulatory Effects and Mechanisms of Curcuma Species and Their Bioactive Compounds: A Review

“…C. zanthorrhiza-inhibited proinflammatory cytokine production in mice induced high-fat diet. C. zanthorrhiza extract at 100 mg/kg body weight/day decreased IL-1β gene expression by 89.9% compared to the control group (Ilene et al, 2020). C. zanthorrhiza was also reported to stimulate total and differential leukocytes in African catfish (Clarias gariepinus) (Lestari et al, 2019).…”

“…It has 2-m tall erect pseudostems (Rajkumari and Sanatombi, 2017) and is generally cultivated in village home gardens. The rhizomes smell balmy and taste bitter (Ilene et al, 2020). C. zanthorrhiza has been used as an active ingredient in cosmetic and hygienic products in Germany and the Netherlands (Drugbank, 2021).…”

“…Volatile oil from C. zanthorrhiza enhanced the lymphocyte proliferation from human male B blood type (Miksusanti, 2012). C. zanthorrhiza extract administration was able to reduce inflammatory lymphocytes in alcohol-induced hepatitis in mice (Ilene et al, 2020). C. zanthorrhiza ethanol extracts strongly reduced cytokine gene expression, which include TNF-α, IL-6, IL-1β, and C-reactive protein (CRP) in the liver, adipose tissue, and muscle of high-fat diet-induced obese mice (Kim M-B et al, 2014).…”

Redefining the hepatoprotective potential of Javanese turmeric (Curcuma xanthorrhiza) Kombucha towards the diethylnitrosamine-induced hepatotoxicity of mice

“…The SGPT and SGOT activity of DEN þ JTK were lower than the DEN þ black tea kombucha group. Javanese turmeric contains xanthorrhizol, curcumin, phenolic compounds and triterpenoids which do not exist in black tea (Puteri et al, 2020). During kombucha fermentation, microbes released these hepatoprotective compounds and produced organic acids such as glucuronic acids and DSL by using sugars added to the substrate (Martínez-Leal et al, 2018).…”

A Comprehensive Review on the Therapeutic Potential of Curcuma longa Linn. in Relation to its Major Active Constituent Curcumin

“…Its medicinally important parts are presented in Figure 1. The rhizomes have a balmy smell and bitter in taste (Puteri et al, 2020). The taxonomic classification of C. longa is shown in Figure 2.…”