Effect of itraconazole on Staphylococcus aureus biofilm and extracellular vesicles formation

Frassinetti, Stefania; Falleni, Alessandra; Carratore, R. Del

doi:10.1016/j.micpath.2020.104267

Cited by 17 publications

(11 citation statements)

References 20 publications

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“…The bacterial biofilm is a complex community formed by extracellular matrix composed of proteins, polysaccharides and extracellular DNA and inner bacterial plankton, which is one of the main drug resistance mechanisms of S.aureus . BBR/BA self-assembly can resist the attack of phagocytic cells or effectively block the infiltration of antibiotics, leading to bacterial drug resistance [ 44 , 45 ]. We further investigated whether thermodynamic process affected the antibacterial activity of phytochemical self-assemblies through the scavenging experiment of bacterial biofilm.…”

Section: Resultsmentioning

confidence: 99%

Thermodynamics driving phytochemical self-assembly morphological change and efficacy enhancement originated from single and co-decoction of traditional chinese medicine

et al. 2022

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Through the self-assembled strategy to improve the clinical efficacy of the existing drugs is the focus of current research. Herbal formula granule is a kind of modern dosage form of traditional Chinese medicine (TCM) which has sprung up in recent decades. However, whether it is equivalent to the TCM decoction that has been used for thousands of years has always been a controversial issue. In this paper, taking the herb pair of Coptidis Rhizoma—Scutellariae Radix and its main component berberine—baicalin as examples, the differences and mechanisms of self-assemblies originated from the co-decoction and physical mixture were studied, respectively. Moreover, the relationship between the morphology and antibacterial effects of self-assemblies was illuminated via multi-technology. Our study revealed that the physical mixture’s morphology of both the herb pair and the phytochemicals was nanofibers (NFs), while their co-decoction’s morphology was nanospheres (NPs). We also found that the antibacterial activity was enhanced with the change of self-assemblies’ morphology after the driving by thermal energy. This might be attributed to that NPs could influence amino acid biosynthesis and metabolism in bacteria. Current study provides a basis that co-decoction maybe beneficial to enhance activity and reasonable use of herbal formula granule in clinic.

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Section: Resultsmentioning

confidence: 99%

Thermodynamics driving phytochemical self-assembly morphological change and efficacy enhancement originated from single and co-decoction of traditional chinese medicine

et al. 2022

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“…According to a previous study, the SEM was used to observe the S. epidermidis cell morphological changes, according to the previous research with modification ( Frassinetti et al, 2020 ). Sterile 14 mm circular slides were placed on 24-well plates and 1 ml of 1 × 10 6 CFU/ml suspension of the test strain was added to each well and incubated at 37°C for 6, 18 and 36 h. Samples were dehydrated in an ascending ethanol series.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial and anti-biofilm activities of Disaspidin BB against Staphylococcus epidermidis

et al. 2023

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IntroductionStaphylococcus epidermidis infections are an important concern in worldwide, especially when associated with biofilms, and resistance of this agent to many drugs makes the situation even worse. We investigated the inhibitory effect of Disaspidin BB obtained from plant extracts and purifications on clinical S. epidermidis strains and their biofilms, and preliminarily investigated its mechanism of of its anti-biofilm activity.Methods and ResultsThe broth dilution method was used to determine the minimum inhibitory concentrations (MIC) of Disaspidin BB on 11 clinical S. epidermidis strains (MIC value of 0.63 ~ 2.5 μg/ml). SEP-05 was found to be erythromycin-resistant (MIC value>8 μg/ml) and Disaspidin BB sensitive with an MIC value of 0.63 μg/ml. The time-kill curve assay indicated that the antibacterial activity of Disaspidin BB against SEP-05 with concentration dependence. The metabolic activity and total biomass of the drug-treated SEP-05 biofilm in each stage were significantly inhibited by the crystalline violet and XTT assay, and the scavenging effect of Disaspidin BB on SEP-05 biofilm was also confirmed by SEM observation. The results of real-time quantitative PCR showed that subinhibitory concentrations Disaspidin BB can inhibit biofilm formation by affecting the expression level of key genes (aap, atlE, icaA, luxS, recA) in SEP-05 biofilm formation. In addition, the content of polysaccharides, proteins and extracellular DNA in biofilm matrix after the intervention of Disaspidin BB was significantly reduced, and it was tentatively determined that the ability of SEP-05 biofilm formation and its stability were thus disturbed.DiscussionThe results show that Disaspidin BB has promising antibacterial effect on erythromycin-resistant S. epidermidis and significant scavenging effect on its biofilm, which provides a theoretical basis for the further development of BB as a new drug for the treatment of skin infections caused by S. epidermidis.

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“…2d) [39], which is composed of polysaccharides, extracellular polymer, lipid, and DNA [40], functioning as a major barrier to the efficient penetration of antimicrobial agents. Some bacteria have a strong capacity to form biofilm s, such as epidermal Staphylococcus aureus [41], and copper-green pseudomonas [42], which adhere to the mucous layer and to each other. Once the biofilm forms, bacteria become resistant to antimicrobials and the body's immune removal.…”

Section: Biofilmmentioning

confidence: 99%

A roadmap to pulmonary delivery strategies for the treatment of infectious lung diseases

Gui

Xiong

et al. 2022

J Nanobiotechnol

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Pulmonary drug delivery is a highly attractive topic for the treatment of infectious lung diseases. Drug delivery via the pulmonary route offers unique advantages of no first-pass effect and high bioavailability, which provides an important means to deliver therapeutics directly to lung lesions. Starting from the structural characteristics of the lungs and the biological barriers for achieving efficient delivery, we aim to review literatures in the past decade regarding the pulmonary delivery strategies used to treat infectious lung diseases. Hopefully, this review article offers new insights into the future development of therapeutic strategies against pulmonary infectious diseases from a delivery point of view. Graphical Abstract

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Effect of itraconazole on Staphylococcus aureus biofilm and extracellular vesicles formation

Cited by 17 publications

References 20 publications

Thermodynamics driving phytochemical self-assembly morphological change and efficacy enhancement originated from single and co-decoction of traditional chinese medicine

Thermodynamics driving phytochemical self-assembly morphological change and efficacy enhancement originated from single and co-decoction of traditional chinese medicine

Antibacterial and anti-biofilm activities of Disaspidin BB against Staphylococcus epidermidis

A roadmap to pulmonary delivery strategies for the treatment of infectious lung diseases

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