Effect of Isoflurane on the Hearing in Mice

Kim, Joung Uk; Ahn, Yun Suk; Suh, Jin Kyung; Chung, Jong Woo

doi:10.7874/kja.2012.16.1.14

Cited by 2 publications

(2 citation statements)

References 19 publications

(15 reference statements)

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“…Our results demonstrate that, in the mid-to high range tones of the cochlea (i.e., 9.2–32 kHz), DPOAE thresholds obtained under isoflurane anesthesia were significantly elevated compared to those obtained under ketamine/xylazine anesthesia. Similarly, Kim and colleagues observed poorer DPOAE thresholds across all frequencies in mice anesthetized with isoflurane (Kim et al, 2012 ). Data obtained by Sheppard colleagues in Sprague-Dawley rats also reported that usage of isoflurane anesthesia could significantly reduce DPOAE amplitudes relative to ketamine/xylazine (Sheppard et al, 2018 ).…”

Section: Discussionmentioning

confidence: 84%

“…A study performed in rats also showed significantly elevated ABR threshold among all frequencies tested (ranging from 8 to 32 kHz) under isoflurane anesthesia relative to ketamine/xylazine anesthesia with the greatest threshold shift found in the region between 12 and 24 kHz (Ruebhausen et al, 2012 ). In contrast, Kim and colleagues found no differences between ABR baseline recordings using BALB/c mice (Kim et al, 2012 ). Our study revealed a significant reduction in wave I amplitude when isoflurane anesthesia was used.…”

Section: Discussionmentioning

confidence: 95%

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Effect of Oral Allylnitrile Administration on Cochlear Functioning in Mice Following Comparison of Different Anesthetics for Hearing Assessment

Verdoodt¹,

Eens²,

Dam³

et al. 2021

Front. Toxicol.

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Background: Allylnitrile is a compound found in cruciferous vegetables and has the same lethality and toxic effects as the other nitriles. In 2013, a viable allylnitrile ototoxicity mouse model was established. The toxicity of allylnitrile was limited through inhibition of CYP2E1 with trans-1,2-dichloroethylene (TDCE). The allylnitrile intoxication model has been extensively tested in the 129S1 mouse strain for vestibular function, which showed significant HC loss in the vestibular organ accompanied by severe behavioral abnormalities. However, the effect of allylnitrile on auditory function remains to be evaluated. Commonly used anesthetics to conduct hearing measurements are isoflurane and ketamine/xylazine anesthesia but the effect of these anesthetics on hearing assessment is still unknown. In this study we will evaluate the otovestibular effects of oral allylnitrile administration in mice. In addition, we will compare the influence of isoflurane and ketamine/xylazine anesthesia on hearing thresholds.Methods and Materials: Fourteen Coch+/– CBACa mice were randomly allocated into an allylnitrile (n = 8) and a control group (n = 6). Baseline measurements were done with isoflurane and 1 week later under ketamine/xylazine anesthesia. After baseline audiovestibular measurements, mice were co-administered with a single dose of allylnitrile and, to reduce systemic toxicity, three intraperitoneal injections of TDCE were given. Hearing loss was evaluated by recordings of auditory brainstem responses (ABR) and distortion product otoacoustic emissions (DPOAE). Specific behavioral test batteries for vestibular function were used to assess alterations in vestibular function.Results: Hearing thresholds were significantly elevated when using isoflurane anesthesia compared to ketamine/xylazine anesthesia for all frequencies of the ABR and the mid-to-high frequencies in DPOAE. Allylnitrile-treated mice lacked detectable ABR thresholds at each frequency tested, while DPOAE thresholds were significantly elevated in the low-frequency region of the cochlea and completely lacking in the mid-to high frequency region. Vestibular function was not affected by allylnitrile administration.Conclusion: Isoflurane anesthesia has a negative confounding effect on the measurement of hearing thresholds in mice. A single oral dose of allylnitrile induced hearing loss but did not significantly alter vestibular function in mice. This is the first study to show that administration of allylnitrile can cause a complete loss of hearing function in mice.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 95%

Effect of Oral Allylnitrile Administration on Cochlear Functioning in Mice Following Comparison of Different Anesthetics for Hearing Assessment

Verdoodt¹,

Eens²,

Dam³

et al. 2021

Front. Toxicol.

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Identifying the Origin of Effects of Contralateral Noise on Transient Evoked Otoacoustic Emissions in Unanesthetized Mice

Xu¹,

Cheatham

Siegel

2017

JARO

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Descending neural pathways in the mammalian auditory system are known to modulate the function of the peripheral auditory system. These pathways include the medial olivocochlear (MOC) efferent innervation to outer hair cells (OHCs) and the acoustic reflex pathways mediating middle ear muscle (MEM) contractions. Based on measurements in humans (Marks and Siegel, companion paper), we applied a sensitive method to attempt to differentiate MEM and MOC reflexes using contralateral acoustic stimulation in mice under different levels of anesthesia. Separation of these effects is based on the knowledge that OHC-generated transient evoked otoacoustic emissions (TEOAE) are delayed relative to the stimulus, and that the MOC reflex affects the emission through its innervation of OHC. In contrast, the MEM-mediated changes in middle ear reflectance alter both the stimulus (with a short delay) and the emission. Using this approach, time averages to transient stimuli were evaluated to determine if thresholds for a contralateral effect on the delayed emission, indicating potential MOC activation, could be observed in the absence of a change in the stimulus pressure. This outcome was not observed in the majority of cases. There were also no statistically significant differences between MEM and putative MOC thresholds, and variability was high for both thresholds regardless of anesthesia level. Since the two reflex pathways could not be differentiated on the basis of activation thresholds, it was concluded that the MEM reflex dominates changes in TEOAEs induced by contralateral noise. This result complicates the identification of purely MOC-induced changes on OAEs in mice unless the MEM reflex is inactivated surgically or pharmacologically.

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Effect of Isoflurane on the Hearing in Mice

Cited by 2 publications

References 19 publications

Effect of Oral Allylnitrile Administration on Cochlear Functioning in Mice Following Comparison of Different Anesthetics for Hearing Assessment

Effect of Oral Allylnitrile Administration on Cochlear Functioning in Mice Following Comparison of Different Anesthetics for Hearing Assessment

Identifying the Origin of Effects of Contralateral Noise on Transient Evoked Otoacoustic Emissions in Unanesthetized Mice

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