This study provides basic data on the growth and production of seedlings produced in plant factories with artificial lighting by comparing seedling quality, growth and fruit characteristics, and production after transplanting cucumber seedlings according to environmental differences between plant factories with artificial lighting and conventional nurseries in greenhouse. The control group consisted of greenhouse seedlings (GH) grown in the conventional nursery before transplanting. Plant factory to greenhouse seedlings (PG) were grown for 9 days in a plant factory with artificial lighting and for 13 days in an conventional nursery. Plant factory seedlings (PF) were grown in a plant factory with artificial lighting for 22 days until planting. In terms of seedling quality, PFs had the highest relative growth rate and compactness and the best root zone development. After transplanting PFs tended to grow faster, the first harvest date was 2 days earlier than that of GHs, and the growing season ended 1 day earlier. The female flower flowering rate of the PFs was high, and the fruit set rate was of PF the lowest. The production per unit area was highest for PFs at 10.23kg Performance index on the absorption basis, the most sensitive chlorophyll fluorescence parameter, was highest at 4.14 for PFs at 4 weeks after transplantation. By comparing the maximum quantum yield of primary PS Ⅱ photochemistry and dissipated energy flux per PS Ⅱ reaction center electron at 4 weeks after transplantation, PFs tended to be the least stressed. PFs had the best seedling quality, growth, and production after planting, and fruit quality was consistent with that of greenhouse seedlings. Therefore, plant factory seedlings can be used in the field.