Effect of iron nanoparticles on free radical oxidation process in blood of rats with Pliss lymphosarcoma

Abstract

The use of metal nanoparticles (NPs) for cancer treatment requires careful examination of their biological effects. The aim of this study was to determine parameters of oxidative processes in the blood of tumor-bearing animals treated with metallic iron NPs only. The markers of antioxidant status and accumulation of lipid peroxidation products were measured in erythrocytes and blood plasma of rats with Pliss lymphosarcoma (PLS) and intact rats. PLS animals were treated eight times with iron NPs (at a dose of 1.25 mg/kg bw (main group), rats of the control group received saline (0.3 ml). In control animals, an increase in malondialdehyde (MDA) was observed in red blood cells (RBC) by 45%; this was accompanied by compensatory increase in reduced glutathione (GSH) and catalase by 24% and 14.3%, respectively (p<0.05). In plasma an increase in MDA by 167.4% (p<0.01) and a decrease in oxidase activity of ceruloplasmin (CP) by 36.8% (p<0.001) were found. In the main group there was a decrease of accumulation of lipid peroxidation products in the blood. Intensity of detected changes depended on the antitumor effect: rats with growing LSP showed a tendency to the decrease in the RBC MDA level and normalization of plasma MDA; in animals with LSP regression this marker did not differ from normal values. In all animals of the main group the CP content was basically the same as in intact rats while GSH increased in the group without therapeutic effect (by 218.6%) and in the group with the effect by 69% (versus normal values; p<0.01). SOD activity in the rats with LSP growth significantly increased (by 42%), in the rats with regression decreased (by 30%) with subsequent normalization. Thus, administration of iron NPs caused activation of the antioxidant system in blood and a significant decrease in the manifestations of oxidative stress associated with tumor growth.

Influence of diabetes mellitus on free radical processes in the heart in rats with Guerin’s carcinoma and characteristics of malignant lesions depending on the gender of animals

“…It can be assumed that one of the factors that ensure the greatest tumor growth in the males with DM is the intensification of lipid peroxidation and the activation of SOD in the tumor tissue, which protects tumor cells from the development of oxidative stress. The dependence of the tumor growth on the oxidative status has been also shown in earlier studies conducted by our Research Center [21][22][23][24]. This mechanism of the tumor progression is discussed by the latest reviews published in the reference papers abroad [25,26].…”

To the question of intensification of free radical oxidation of biosubstrates under action of nanosized materials

“…НЧ металів утворюють функціональні групи з біомолекулами, змінюючи їхню конфігурацію [6], а контакт з клітинами призводить до порушень у тканинах і органах. До найважливіших факторів пошкоджуючої дії НЧ металів відносять їхню здатність посилювати продукцію активних форм кисню (АФК) та азоту (АФА), що може стати причиною пошкодження клітин і тканин організму [6][7][8].…”

“…Ендогенний NO після складного каскаду перетворень переходить у стабільні сполуки -нітрати, нітрити, нітрозотіоли та нітротирозин. NO, будучи малим парамагнітним радикалом, що не має електричного заряду, легко проходить крізь клітинні мембрани, добре розчиняється у воді та ліпідах, вступає в реакції з іншими молекулами на значній відстані від місця його утворення та може впливати на метаболічні процеси як у клітинах синтезу, так і рядом розташованих [7]. NO являє собою активний, короткоживучий радикал (період існування від 3 до 50 діб).…”