Effect of Iron Limitation on Slime Production by Staphylococcus aureus

Baldassarri, Lucilla; Bertuccini, L.; Ammendolia, Maria Grazia; Arciola, Carla Renata; Montanaro, Lucio

doi:10.1007/s100960100495

Cited by 9 publications

(5 citation statements)

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“…The expression of biofilm is also regulated by iron, with maximum expression occurring at low concentrations. 29,30 As compliance with hand hygiene rarely exceeds 50%, 31 Tvedt et al suggested that hand disinfection may reduce the normal bacterial flora but its ability to inactivate potential pathogenic bacteria was not examined. 32 The persistance of S. epidermidis in biomaterial was explained by the theory that some microorganisms may be protected from disinfectant action by creation of localized micro-environments of reduced disinfectant concentration due to organic matter acting as a sink for the antimicrobial agents.…”

Section: Discussionmentioning

confidence: 98%

Detection of icaA and icaD loci by polymerase chain reaction and biofilm formation by Staphylococcus epidermidis isolated from dialysate and needles in a dialysis unit

Chaieb¹,

Mahdouani²,

Bakhrouf³

2005

Journal of Hospital Infection

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Section: Discussionmentioning

confidence: 98%

Detection of icaA and icaD loci by polymerase chain reaction and biofilm formation by Staphylococcus epidermidis isolated from dialysate and needles in a dialysis unit

Chaieb¹,

Mahdouani²,

Bakhrouf³

2005

Journal of Hospital Infection

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“…The expression of the ica locus and biofilm formation is highly variable and can be induced by variations in the culture conditions of CNS, such as media used, minor differences in media composition and source, such as the manufacturer [27], low concentrations of iron [21,28], concentration of sugars, sodium chloride, and ethanol [3,29], or other substances that induce stress conditions [30,31], which, in turn, stimulate or inhibit fermentation, thus increase or depress the production of PIA, with a consequent impact on biofilm production [32]. Therefore, variations in the milk composition during the lactation period could hypothetically induce a significant effect on biofilm formation [33].…”

Section: Discussionmentioning

confidence: 99%

Relevance of biofilm formation and virulence of different species of coagulase-negative staphylococci to public health

Osman

El-Razik²,

Marie³

et al. 2015

Eur J Clin Microbiol Infect Dis

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The ability of biofilm formation seems to play an essential role in the virulence of coagulase-negative staphylococci (CNS). The present work aimed to: (a) evaluate the biofilm-forming ability of different strains of CNS field isolates; (b) evaluate their virulence potential through the assessment of the Madin-Darby canine kidney (MDCK) cytotoxicity assay; (c) determine the frequency of biofilm-associated genes among these CNS isolates. Biofilm markers associated with biofilm formation and MDCK cells cytotoxicity were compared to find possible associations with pathogenicity. CNS isolates (n = 94) belonging to 11 different species were tested for slime production using the tube test (TA) and the Congo red agar plate test (CRA), while the presence of icaA and icaD genes were evaluated by d-PCR. Two points were addressed for the first time: (1) the specific relationship between slime phenotype and icaD gene expression; (2) the specific relationship between slime phenotype, icaAD genes, and MDCK cytotoxicity. The proportion of biofilm-positive/icaD-positive versus biofilm-positive/icaD-negative strains was 9:0 and 9:0 (81.8%) by the TA and CRA, which clearly indicates that icaD was a more reliable gene to be accounted for in the biofilm formation. MDCK recorded a higher proportion than that recorded by the CRA and TA results (MDCK-positive/icaD-positive versus MDCK-positive/icaD-negative 10:0, 90.9%). Evaluation of the ica operon, CRA plate test, TA, and MDCK can contribute to the high clinical impact in the management of antibiotic therapy, in infections associated with devices in veterinary medicine, the dairy industry, and food processing.

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“…Because the expression of adherence factors by several bacterial species is influenced by the iron supply in the environment, the present study demonstrated the effects of the presence of the iron chelator dipyridyl on the ability of EAEC strains to form biofilms in polystyrene plates and their ability to attach to HEp-2 cells. Although iron chelating compounds are not completely specific for iron and may also disrupt the homeostasis of other essential metals, 2,2-dipyridyl is a potent chelator of iron that has been currently used in studies dealing with the influence of iron limiting conditions on virulence of micro-organisms (Deighton and Borland 1993;Baldassarri et al 2001;Morrissey et al 2002;Moreira et al 2003;Wu and Outten 2009), and in determination of the iron content in intact cells (Petrat et al 2000).…”

Section: Discussionmentioning

confidence: 99%

“…The expression of adherence factors by several bacterial species is influenced by the iron supply in the environment (Deighton and Borland 1993;Baldassarri et al 2001;Morrissey et al 2002;Moreira et al 2003). Okeke et al (2004) have reported the association of iron utilization genes with disease in EAEC.…”

Section: Introductionmentioning

confidence: 99%

Iron-limited condition modulates biofilm formation and interaction with human epithelial cells of enteroaggregative Escherichia coli (EAEC)

Alves

Pereira

Souza

et al. 2010

Journal of Applied Microbiology

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Aims: The aim of this study was to investigate the influence of low iron availability on biofilm formation and adherence to HEp‐2 cells of enteroaggregative Escherichia coli (EAEC) strains isolated from diarrhoea cases. Methods and Results: The ability of EAEC to form biofilm on a plastic surface was evaluated quantitatively and qualitatively after 3 and 18 h of incubation of strains with or without the iron chelator 2,2‐dipyridyl. When submitted to low iron conditions, prototype EAEC 042 strain showed a decrease in biofilm formation. Conversely, an increase in biofilm formation was observed for the clinical EAEC strains cultured in restricted iron condition. Moreover, the reduction of iron concentration inhibited the aggregative adherence to HEp‐2 cells of all EAEC strains tested. However, all effects promoted by iron chelation were suppressed by thiourea. Conclusions: Low iron availability may modulate biofilm formation and adhesive properties of EAEC strains to HEp‐2 cells. Significance and Impact of the Study: The data obtained in this study provide useful insights on the influence of low iron conditions possibly associated with redox stress on the pathogenesis of EAEC strains.

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Effect of Iron Limitation on Slime Production by Staphylococcus aureus

Cited by 9 publications

References 0 publications

Detection of icaA and icaD loci by polymerase chain reaction and biofilm formation by Staphylococcus epidermidis isolated from dialysate and needles in a dialysis unit

Detection of icaA and icaD loci by polymerase chain reaction and biofilm formation by Staphylococcus epidermidis isolated from dialysate and needles in a dialysis unit

Relevance of biofilm formation and virulence of different species of coagulase-negative staphylococci to public health

Iron-limited condition modulates biofilm formation and interaction with human epithelial cells of enteroaggregative Escherichia coli (EAEC)

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