Effect of Iron Chloride on Peroxidase Activity in Kidney and Liver of Labeo rohita

Nawaz, Rimsha

doi:10.17582/journal.pjz/2018.50.1.sc4

Cited by 3 publications

(2 citation statements)

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“…it was observed that though there were reduced enzyme activities after the optimum concentration of ferric chloride was reached, all concentrations of ferric chloride activated the enzyme. The observations in this study are similar to the findings in previous research on the effect of iron chloride on peroxidase enzyme activity in the fish Labeo rohita [12] as the results showed that peroxidase activity was increased significantly in both kidneys and liver after exposure to iron chloride. Fig.…”

Section: Resultssupporting

confidence: 91%

Evaluation of the Effects of Some Metal Chlorides on the Initial Reaction Rate of Crude Peroxidase from Watermelon Peels

Iniaghe,

Ibukun

2023

AJB2T

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Aims: This study evaluates the effect of some chloride on the initial reaction rates of crude peroxidase from watermelon peels. Study Design: In vitro enzyme assay. Place and Duration of Study: Department of Biochemistry, Faculty of Life Sciences, Ambrose Alli University, Ekpoma, Edo State, Nigeria between April 2021 and June 2021. Methodology: The kinetics of crude peroxidase catalyzed oxidation of 3,5,3′,5′-tetramethylbenzidine (TMB) in the presence of varying concentrations of different chloride salts and hydrogen peroxidase was determined spectrophotometrically at 655nm. The assay mixture contained 2.3 mL of sodium phosphate buffer of pH 7.0, 0.1 mL of a crude enzyme from the watermelon peels, 0.2 mL of varying concentration of the respective chloride salts, 0.2 mL of 0.02 mM TMB, and 0.2 mL of 2 mM hydrogen peroxidase added last to start the reaction. Results: Results showed that chloride salts of Pb2+ Hg2+ Fe2+ and Na+ had peroxidase activating effects at low salt concentration (1 mM). The activating effect increased proportionately up to their respective optimum salt concentration. Further increment of salt concentration above their respective optimum value lead to a decrease in initial reaction rate of the crude enzyme. Within a chloride concentration range of 0.025 mM to 3 mM, the highest activating effect recorded was in the order Pb2+ > Fe2+ > Na+ > Hg2+.. Results show that Pb2+ had the highest enzyme activating effect at the lowest concentration (0.5 mM) of the salt when compared with other salts within the same concentration range. Conclusion: These findings are of great significance in research to understanding the mechanism of action of peroxidase from the peels of watermelon, especially as the search for cheap and alternative sources of peroxidases continues.

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Section: Resultssupporting

confidence: 91%

Evaluation of the Effects of Some Metal Chlorides on the Initial Reaction Rate of Crude Peroxidase from Watermelon Peels

Iniaghe,

Ibukun

2023

AJB2T

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show abstract

“…The observations in this study are similar to the findings in previous research on the effect of iron chloride on peroxidase enzyme activity in the fish Labeo rohita [15] as the results showed that peroxidase activity was increased significantly in both kidneys and liver after exposure to iron chloride. Other researchers [16] reported that the levels of antioxidant enzyme "peroxidase" increased with iron concentration.…”

Section: Resultssupporting

confidence: 91%

Effects of Chlorides of Lead and Some Transition Metals on the Kinetics of Crude Peroxidase from Watermelon Seeds

Iniaghe

Adeyemi

2021

JABB

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Aims: This study investigates the effect of chlorides of lead and some transition metals on the kinetics of crude peroxidase from watermelon seeds Study design: In vitro enzyme assay. Place and Duration of Study: Department of Biochemistry, Faculty of Life Sciences, Ambrose Alli University, Ekpoma, Edo State, Nigeria between April 2021 and June 2021 Methodology: The kinetics of crude peroxidase catalyzed oxidation of 3,5,3′,5′-tetramethylbenzidine (TMB) in the presence of varying concentrations of different chloride salts and hydrogen peroxidase was determined spectrophotometrically at 655nm. The assay mixture contained 2.3 mL of sodium phosphate buffer of pH 7.0, 0.1 mL of the crude enzyme from the seeds of watermelon, 0.2 mL of varying concentration of the respective chloride salts, 0.2 mL of 0.02 mM TMB, and 0.2 mL of 2 mM hydrogen peroxidase added last to start the reaction. Results: Results showed that except for nickel chloride, chloride salts of Pb2+, Hg2+, and Fe2+ had peroxidase activating effects. Mercury chloride and lead chloride proportionately increased the enzyme activity within a salt concentration range of 1.5 and 3 mM. In comparison, ferric chloride had an optimum concentration of 2.5 mM for peroxidase activation; mercury chloride had the highest peroxidase activation effect compared with chlorides of Pb, Ni, and Fe. Conclusion: These findings are of great importance to industries in understanding the mechanism of action of peroxidase from the seeds of watermelon, especially as the search for cheap and alternative sources of peroxidases continues.

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Physiological Responses of the Goldfish (Carassius auratus) During Subacute Exposure to Organic Pollutants

Ebrahimzadeh

Heidari

Nazarhaghighi

et al. 2021

Bull Environ Contam Toxicol

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Effect of Iron Chloride on Peroxidase Activity in Kidney and Liver of Labeo rohita

Cited by 3 publications

References 0 publications

Evaluation of the Effects of Some Metal Chlorides on the Initial Reaction Rate of Crude Peroxidase from Watermelon Peels

Evaluation of the Effects of Some Metal Chlorides on the Initial Reaction Rate of Crude Peroxidase from Watermelon Peels

Effects of Chlorides of Lead and Some Transition Metals on the Kinetics of Crude Peroxidase from Watermelon Seeds

Physiological Responses of the Goldfish (Carassius auratus) During Subacute Exposure to Organic Pollutants

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