Effect of iron and growth inhibitors on siderophores production by Pseudomonas fluorescens

Rachid, Djibaoui; Bensoltane, Ahmed

doi:10.5897/ajb2005.000-3129

Cited by 65 publications

(21 citation statements)

References 24 publications

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“…King B was the best medium for obtaining the highest value of siderophore. Similarly Rachid and Ahmed (2005) found that king B medium is better than several other media. Many studies indicated siderophore production by several fungi such as Aspergillus terreus (Waqas et al, 2015), Aspergillus fumigatus (Haas, 2014), and Chaetostylum fresenii (Thieken and Winkelmann, 1992).…”

Section: Discussionmentioning

confidence: 91%

“…Four media were prepared to detect siderophore production by 65P and 9F strains including: King B medium consisting of glycerine 10 g/L, proteose-peptone 20 g/L, and MgSO4 1.5 g/L (de Villegas et al, 2002), glucose medium consisting of K 2 HPO 4 0.56 g/L, Glucose 10 g/L, urea 0.85 g/L, and Glutamic acid 1 g/L (de Villegas et al, 2002), citrate medium consisting of K 2 HP0 4 6.0 g/L, KH2P04 3.0 g/L, (NH 4 )2S0 4 1.0 g/L, MgS0 4 .7H 2 0 0.2 g/L, and citric acid 4.0 g/L (Meyer and Abdallah, 1978) and asparagine medium consisting of Asparagine 5 g/L, MgSO 4 0.1 g/L, and K 2 HPO 4 0.5 g/L (Rachid and Ahmed, 2005). The pH for all the media was adjusted to 7.0.…”

Section: Methodsmentioning

confidence: 99%

“…The concentration was calculated according to method of Meyer and Abdallah (1978) using absorption maximum (λ = 400 nm) and molar extinction coefficient ε = 20000 (Rachid and Ahmed, 2005). …”

Section: Methodsmentioning

confidence: 99%

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Aspergillus terreus Inhibits Growth and Induces Morphological Abnormalities in Pythium aphanidermatum and Suppresses Pythium-Induced Damping-Off of Cucumber

2018

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The study investigated the efficacy of two isolates of Aspergillus terreus (65P and 9F) on the growth, morphology and pathogenicity of Pythium aphanidermatum on cucumber. In vitro tests showed that the two isolates inhibited the growth of P. aphanidermatum in culture. Investigating P. aphanidermatum hyphae close to the inhibition zone showed that the hyphae showed abnormal growth and loss of internal content. Treating P. aphanidermatum with the culture filtrate (CF) of A. terreus resulted in significant rise in cellular leakage of P. aphanidermatum mycelium. Testing glucanase enzyme activity by both A. terreus isolates showed a significant increase in glucanase activity. This suggests that the cell walls of Pythium, which consist of glucan, are affected by the glucanase enzyme produced by A. terreus. In addition, Aspergillus isolates produced siderephore, which is suggested to be involved in inhibition of Pythium growth. Also, the CFs of 65P and 9F isolates significantly reduced spore production by P. aphanidermatum compared to the control (P < 0.05). In bioassay tests, the two isolates of A. terreus increased the survival rate of cucumber seedlings from 10 to 20% in the control seedlings treated with P. aphanidermatum to 38–39% when the biocontrol agents were used. No disease symptoms were observed on cucumber seedlings only treated with the isolates 65P and 9F of A. terreus. In addition, the A. terreus isolates did not have any negative effects on the growth of cucumber seedlings. This study shows that isolates of A. terreus can help suppress Pythium-induced damping-off of cucumber, which is suggested to be through the effect of A. terreus and its glucanase enzyme on P. aphanidermatum mycelium.

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Section: Discussionmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

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Aspergillus terreus Inhibits Growth and Induces Morphological Abnormalities in Pythium aphanidermatum and Suppresses Pythium-Induced Damping-Off of Cucumber

2018

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“…Similar observations have been made for Pseudomonas sp. strains by Sharma and Johri (2003) and for P. fluorescens by Djibaoui and Bensoltane (2005). In addition, cell growth and siderophore production were inversely proportional responses.…”

Section: Discussionmentioning

confidence: 96%

Evaluation of rhizospheric Pseudomonas and Bacillus as biocontrol tool for Xanthomonas campestris pv campestris

Mishra

Arora

2011

World J Microbiol Biotechnol

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Xanthomonas campestris pv campestris (Xcc), causing black rot, is one of the most yield-limiting and destructive pathogens of cruciferous crops. The intention of this study was to evaluate the potential of rhizobacteria in black rot management. Fifty-four isolates from rhizosphere soil of Brassica campestris were screened against Xcc. Two isolates namely, KA19 and SE, with inhibition radius >11 mm were selected. The combined use of them produced an average inhibition zone of 18.1 ± 1.4 mm radius (P < 0.05). 16S rRNA gene sequencing and phylogenetic analysis identified KA19 and SE as the nearest homologs (>99.4%) of Pseudomonas aeruginosa and Bacillus thuringiensis, respectively. In greenhouse study, both isolates were effective (P < 0.05) in reducing black rot lesions compared to untreated control involving either a foliar spray or the combined seed soak and soil drench. However, the combined strains (KA19 + SE) were significantly more effective (P < 0.05) when the mode of application was combined seed and soil drench. The lipid content of seeds increased significantly with the application of these strains, especially with SE alone and in combination. After 9 weeks, the Xcc population was significantly lower in soil treated with combined strains (P < 0.05). KA19 produced extracellular siderophores, influenced by various carbon sources and identified as 4-hydroxy-2-nonyl-quinoline by NMR. In Bacillus SE, two antibacterial factors corresponding to autolysins (β-N-acetylglucosaminidase) and AHL-lactonases were established. This study would strengthen our understanding for application of different rhizobacteria with various active principles like Pseudomonas and Bacillus as ingredients of a biocontrol mixture.

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“…Results show that pyoverdine production was inversely related to Fe 3+ concentration, whereas growth was directly proportional to iron concentration (Meyer et al, 1978;Visca et al, 1992;Villegas et al, 2002;Manwar et al, 2004). Djibaoui and Bensoltane (2005) observed a complete decline of siderophore production with 200 µg/L of iron as threshold level. This fact reflects the iron requirement for microbiological cellular processes (Sayyed et al, 2005).…”

Section: +mentioning

confidence: 95%

Effect of Dose-Response of Zinc and Manganese on Siderophores Production

Mehri¹,

Khessairi²,

Turki³

et al. 2012

American Journal of Environmental Sciences

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Problem statement:This study was conducted to find and determine whether the siderophores of the four environmental Pseudomonas spp. isolates possess a sequestering activity towards essential transition metals (Zn and Mn) other than iron. Approach: Four fluorescent Pseudomonads isolated from various environments, were characterized analytically (Isoelectric focusing), biologically (pyoverdine-mediated uptake) and genetically (16S rDNA sequencing). By means of spectrophotometric measurements, it was possible to establish and compare the levels of pyoverdine production, in two different nutrient-poor media. Results: The strains were assigned, by sequencing, to P. fluorescens, P. aeruginosa, P. putida and P. mosselii isolated, respectively from soil, compost, sea water and waste water treatment plant. These bacterial strains were recognized as producing diver's yellow-green siderophores types, when grown under conditions of iron starvation. The highest metabolite concentration was obtained with PsC132 and PsTp171 strains isolated respectively from compost and waste water treatment plant, in CAA medium. Strains grown in CAA medium exhibit a higher PVD level compared to SM medium. Mn (II) was found to promote pyoverdine biosynthesis, but rather, Zn (II) had no significant effect on siderophore production when compared to control medium. For both strains PsS29 and PsC132, the increase of iron concentration quenched siderophore production especially above 20 µM. Pyoverdine level declined with the high concentration of zinc but increased with Manganese concentration ranging up to 70 µM (in case of PsC132) and 300 µM (in case of PsS29). Conclusion/Recommendations: The ability of fluorescent Pseudomonas, isolated from wastewater treatment plant and from compost, to sequester zinc, point to a unique advantage of these species for divers bioremediation applications.

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Effect of iron and growth inhibitors on siderophores production by Pseudomonas fluorescens

Cited by 65 publications

References 24 publications

Aspergillus terreus Inhibits Growth and Induces Morphological Abnormalities in Pythium aphanidermatum and Suppresses Pythium-Induced Damping-Off of Cucumber

Aspergillus terreus Inhibits Growth and Induces Morphological Abnormalities in Pythium aphanidermatum and Suppresses Pythium-Induced Damping-Off of Cucumber

Evaluation of rhizospheric Pseudomonas and Bacillus as biocontrol tool for Xanthomonas campestris pv campestris

Effect of Dose-Response of Zinc and Manganese on Siderophores Production

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