Effect of ionizing radiation after-therapy interval on bone: histomorphometric and biomechanical characteristics

Soares, Priscilla Barbosa Ferreira; Soares, Carlos José; Limirio, Pedro Henrique Justino Oliveira; Jesus, Rainde Naiara Rezende de; Dechichi, Paula; Spin‐Neto, Rubens; Zanetta‐Barbosa, Darceny

doi:10.1007/s00784-018-2724-3

Cited by 19 publications

(22 citation statements)

References 35 publications

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“…This higher dose increases the risk of ORN (Dieleman et al, 2017) when compared with doses of 50-70 Gy, indicated for cancer treatment by multifractionated delivery (Ohrnell et al, 1997;Lerouxel et al, 2009). Thus, the single dose of 30 Gy was sufficient to cause bone damage, allowing the repair of bone in poor conditions to be evaluated (Batista et al 2014, Rocha et al, 2017Soares et al, 2018;Limirio et al, 2019). Although higher radiation doses would increase the probability of ORN occurring (Lerouxel et al, 2009), surprisingly, in our study no animals developed osteonecrosis.…”

Section: Discussionmentioning

confidence: 46%

“…Deleterious effects of ionizing radiation on bone tissue can persist for long periods and the complications in irradiated bone appear to be dose dependent (Lerouxel et al, 2009;Limírio et al, 2019). The single dose of 30 Gy radiation has previously been used in other studies of our group (Batista et al 2014, Rocha et al, 2017Soares et al, 2018;Limirio et al, 2019). This higher dose increases the risk of ORN (Dieleman et al, 2017) when compared with doses of 50-70 Gy, indicated for cancer treatment by multifractionated delivery (Ohrnell et al, 1997;Lerouxel et al, 2009).…”

Section: Discussionmentioning

confidence: 94%

“…These results are conflicting with others who have demonstrated improved bone regeneration (Park et al, 2019) and angiogenesis (An et al, 2019) in irradiated (12Gy single dose) calvaria defects after HBO treatment. This most probably occurred due to different experimental models, considering that damage by radiation occurs in a dose-dependent manner (Lerouxel et al, 2009;Limirio et al, 2019;Bai et al, 2020). To our knowledge, the main target for the physiological action of HBO is tissue hypoxia (Choudhury et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

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Influência da oxigenação hiperbárica no reparo ósseo e organização do colágeno após radioterapia

Rocha¹,

Rezende²,

Reis³

et al. 2020

RSD

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O presente estudo teve como objetivo avaliar os efeitos da HBO no reparo ósseo após radiação ionizante, por meio da histomorfometria, tomografia computadorizada e microscopia de polarização. Vinte ratos Wistar machos foram usados. Uma dose de radiação (30 Gy) foi administrada na perna esquerda em todos os animais, e após 30 dias, defeitos ósseos foram criados em ambos os fêmures. Em seguida, 10 animais receberam sessões diárias de HBO (2,5 ATA por 90 minutos), e todos os animais foram sacrificados 5 ou 7 dias após a cirurgia. Os fêmures foram separados em 4 grupos (n = 5) para cada intervalo de tempo de eutanásia: Controle (fêmur direito: não irradiado e não HBO), RXT (fêmur esquerdo: irradiado e não HBO), HBO (fêmur direito: não-irradiado com HBO), RXT + HBO (fémur esquerdo: irradiado com HBO). A cortical óssea e os defeitos foram avaliados. Os dados foram analisados por meio dos testes de Kolmogorov-Smirnov, teste t não pareado e ANOVA com correção de Bonferroni. Valores maiores de HU e neoformação óssea foram observados nos grupos Controle e HBO, com melhora do reparo. O grupo HBO apresentou birrefringência de colágeno predominantemente amarela/laranja/vermelha na área do defeito. A HBO melhorou a reparação óssea em condições fisiológicas, com aumento dos vasos sanguíneos e formação óssea. No entanto, não foi eficiente em melhorar o reparo e a orientação do colágeno no osso após altas doses de radiação ionizante.

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Section: Discussionmentioning

confidence: 46%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

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Influência da oxigenação hiperbárica no reparo ósseo e organização do colágeno após radioterapia

Rocha¹,

Rezende²,

Reis³

et al. 2020

RSD

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“…BMD is an important choice for osteoporosis assessment; the changes in BMD affect bone strength [ 23 ]. Bone biomechanics is a direct and reliable method for evaluating bone quality by studying the mechanical properties of bone under external force and the biological effects after stress [ 24 ]. mCT, a non-destructive technology that provides three-dimensional (3D) images of bones in vitro, is often used as an aid to assess bone quality from both macroscopic and microscopic perspectives [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

Systemic osteoprotective effects of Epimedii Folium and Ligustri Lucidi Fructus in senile osteoporosis rats by promoting the osteoblastogenesis and osteoclastogenesis based on MLP-ANN model

Tang

Yingying

et al. 2020

Chin Med

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Background: Senile osteoporosis (SOP), which is caused by unbalanced bone remodeling, leads to significant economic and societal burdens globally. The combination of Epimedii Folium (EF) and Ligustri Lucidi Fructus (LLF) serves as a commonly-used prescription for SOP in Traditional Chinese Medicine (TCM). This study aimed to evaluate the osteoprotective effects of EF and LLF in combination on SOP rats based on the constructed multilayer perception (MLP)-artificial neural network (ANN) model. Methods: 15 month old male Sprague-Dawley rats were administrated with EF, LLF or the combination of EF and LLF (EF&LLF) for 2 months, while 17 month old rats were used as the aging control group. All the rats were anesthetized with 25% ethyl carbamate, then their serum liver and bone tissues were taken. We detected bone mass, bone mineral density (BMD), biomechanics and the microstructure of bone trabecula by micro-CT and H&E staining to evaluate the degree of osteoporosis. Blood lipids and serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ-glutamyl transferase (GGT) and liver pathology were use to assess the side effects of drugs. Levels of alkaline phosphatase (ALP) and Tartrate-resistant acid phosphatase (TRACP) and the ratio of ALP to TRACP both in serum and bone were measured for the evaluation of bone turnover rate. The bone mRNA and protein expression of osteoprotegerin (OPG), nuclear factor-kappa B ligand (RANKL), macrophage colony-stimulating factor (M-CSF), d2 isoform of vacuolar (H +) ATPase (ATP6V0d2), insulin-like growth factor (IGF-1), bone morphogenetic protein-2 (BMP2), M-CSF, Wnt5a, transforming growth factor-β1 (TGF-β1) were detected for evaluating bone metabolism. Results: The results showed that EF&LLF improved bone mass and bone quality by preventing bone loss, increasing maximal load as well as protecting the micro-structural retrogressive change of trabecular bone in SOP rats; ameliorated the steatosis in the liver and decreased blood lipids and serum ALT, AST and GGT; enhanced bone remodeling by stimulating the expression of ALP and TRACP. At the molecular levels, EF&LLF stimulated the osteoclastogenesis by upregulating the protein and mRNA expression of OPG, RANKL, M-CSF and ATP6V0d2; meanwhile, EF&LLF stimulated

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“…Both hind legs of each rabbit were subjected to irradiation using a single dose of 30 Gy. 11 , 12 , 17 A 5-mm bolus was given to ensure full build-up. The tibia metaphysis region of the hind leg was the designated zone for irradiation.…”

Section: Methodsmentioning

confidence: 99%

Biomechanical and morphological changes produced by ionizing radiation on bone tissue surrounding dental implant

Soares

Limirio

et al. 2020

J. Appl. Oral Sci.

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Biomechanical and morphological changes produced by ionizing radiation on bone tissue surrounding dental implant Objective: This study analyzed the effect of ionizing radiation on bone microarchitecture and biomechanical properties in the bone tissue surrounding a dental implant. Methodology: Twenty rabbits received three dental morse taper junction implants: one in the left tibia and two in the right tibia. The animals were randomized into two groups: the nonirradiated group (control group) and the irradiated group, which received 30 Gy in a single dose 2 weeks after the implant procedure. Four weeks after the implant procedure, the animals were sacrificed, and the implant/bone specimens were used for each experiment. The specimens (n=10) of the right tibia were examined by microcomputed tomography to measure the cortical volume (CtV, mm 3), cortical thickness (CtTh, mm) and porosity (CtPo, %). The other specimens (n=10) were examined by dynamic indentation to measure the elastic modulus (E, GPa) and Vickers hardness (VHN, N/mm 2) in the bone. The specimens of the left tibia (n=10) were subjected to pull-out tests to calculate the failure load (N), displacement (mm) up to the failure point and interface stiffness (N/mm). In the irradiated group, two measurements were performed: close, at 1 mm surrounding the implant surface, and distant, at 2.5 mm from the external limit of the first measurement. Data were analyzed using one-way ANOVA, Tukey's test and Student's t-test (α=0.05). Results: The irradiated bone closer to the implant surface had lower elastic modulus (E), Vickers hardness (VHN), Ct.Th, and Ct.V values and a higher Ct.Po value than the bone distant to the implant (P<0.04). The irradiated bone that was distant from the implant surface had lower E, VHN, and Ct.Th values and a higher Ct.Po value than the nonirradiated bone (P<0.04). The nonirradiated bone had higher failure loads, displacements and stiffness values than the irradiated bone (P<0.02). Conclusion: Ionizing radiation in dental implants resulted in negative effects on the microarchitecture and biomechanical properties of bone tissue, mainly near the surface of the implant.

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Effect of ionizing radiation after-therapy interval on bone: histomorphometric and biomechanical characteristics

Cited by 19 publications

References 35 publications

Influência da oxigenação hiperbárica no reparo ósseo e organização do colágeno após radioterapia

Influência da oxigenação hiperbárica no reparo ósseo e organização do colágeno após radioterapia

Systemic osteoprotective effects of Epimedii Folium and Ligustri Lucidi Fructus in senile osteoporosis rats by promoting the osteoblastogenesis and osteoclastogenesis based on MLP-ANN model

Biomechanical and morphological changes produced by ionizing radiation on bone tissue surrounding dental implant

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