Effect of Ionic Strength on the Self-Assembly in Mixtures of Phosphatidylcholine and Sodium Cholate

Meyuhas, Dina; Bor, A.; Pinchuk, Ilya; Kaplun, Alon; Talmon, Yeshayahu; Kozlov, Michael M.; Lichtenberg, Dov

doi:10.1006/jcis.1997.4764

Cited by 31 publications

(32 citation statements)

References 41 publications

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“…A similar lack of dependence on ionic strength was recently reported by Meyuas et al for micellar solutions of sodium cholate and lecithin (23). In line with their findings our phase studies suggest that steric interactions also dominate electrostatic interactions in mixed micelles of NaC and GMO.…”

Section: Resultssupporting

confidence: 89%

Phase Behavior and Aggregate Structure in Aqueous Mixtures of Sodium Cholate and Glycerol Monooleate

Gústafsson

Nylander

Almgren

et al. 1999

Journal of Colloid and Interface Science

102

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Section: Resultssupporting

confidence: 89%

Phase Behavior and Aggregate Structure in Aqueous Mixtures of Sodium Cholate and Glycerol Monooleate

Gústafsson

Nylander

Almgren

et al. 1999

Journal of Colloid and Interface Science

102

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“…This can be explained by a reduction of the surface cross-sectional area (of both the phospholipids and the detergent) upon increasing salt concentration by screening of the electrostatic effect, which will lead to an increased packing parameter. In stage II of the solubilization this will give at low salt worm-like micelles whereas at high salt open bilayers are formed as was reported before [35]. An increase of the overall packing parameter could in this case also be put into effect by an increase of the amount of DOPE, which has a packing parameter larger than 1.…”

Section: Discussionmentioning

confidence: 56%

Two distinct mechanisms of vesicle-to-micelle and micelle-to-vesicle transition are mediated by the packing parameter of phospholipid–detergent systems

Stuart

Boekema

2007

Biochimica et Biophysica Acta (BBA) - Biomembranes

104

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The detergent solubilization and reformation of phospholipid vesicles was studied for various detergents. Two distinct mechanisms of vesicle-to-micelle and micelle-to-vesicle transition were observed by turbidimetry and cryo-electron microscopy. The first mechanism involves fast solubilization of phospholipids and occurs via open vesicular intermediates. The reverse process, micelle-to-vesicle transition, mimics the vesicle-to-micelle transition. In the second mechanism the solubilization is a slow process that proceeds via micelles that pinch off from closed vesicles. During vesicle reformation, the micelle-to-vesicle transition, a large number of densely packed multilamellar vesicles are formed. The route used, for solubilization and reformation, by a given detergent-phospholipid combination is critically dependent on the overall packing parameter of the detergent-saturated phospholipid membranes. By a change of the overall packing parameter the solubilization and or reformation mechanism could be changed. All five detergents tested fit within the proposed model. With two detergents the mechanism could be changed by changing the phospholipid composition or the medium conditions.

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“…Various methods are applied to study the aggregation behaviour of mixed lipid-detergent systems and MVT processes: time-resolved fluorescence (Das, 1996;Seras 1994), cryotransmission electron microscopy (Meyuhas, 1997;Silvanter, 1996;Seras, 1996), static and dynamic light scattering (Miguel, 1989;Polozova, 1995;Forte, 1998;Egelhaaf 1994), gel exclusion chromatography (Lesieur, 1990), differential scanning calorimetry (Otten, 1995;Forte 1998), NMR (Otten, 1995;Polozova, 1993). Compared to light or neutron scattering (Long, 1994;Pedersen, 1995;Hjelm, 1992;Kiselev, 1999) small-angle X-ray scattering (SAXS) is generally not considered as a convenient technique for phospholipid vesicle characterisation (Van Zanten, 1996).…”

Section: Introductionmentioning

confidence: 99%

Temperature-induced micelle to vesicle transition: kinetic effects in the DMPC/NaC system

Lesieur¹,

Киселев²,

Barsukov³

et al. 2000

J Appl Cryst

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The formation of spherical and monodisperse small unilamellar vesicles is observed upon temperature jumps in a mixed system composed of a phospholipid (dimyristoylphosphatidylcholine) and a bile salt (sodium cholate) in an aqueous buffer. In order to enhance the X-ray contrast of the system a mixed water/sucrose buffer is used. The spontaneous formation of unilamellar structures from mixed micelles upon temperature increase and the reverse solubilisation of membranes upon cooling is studied by means of synchrotron radiation small-angle X-ray scattering. The variations of the vesicle size are presented when the temperature and the surfactant / lipid ratio are modified. The kinetic conditions leading to monodisperse vesicles as well as the reversibility of the micelle to vesicle transition are investigated.

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Effect of Ionic Strength on the Self-Assembly in Mixtures of Phosphatidylcholine and Sodium Cholate

Cited by 31 publications

References 41 publications

Phase Behavior and Aggregate Structure in Aqueous Mixtures of Sodium Cholate and Glycerol Monooleate

Phase Behavior and Aggregate Structure in Aqueous Mixtures of Sodium Cholate and Glycerol Monooleate

Two distinct mechanisms of vesicle-to-micelle and micelle-to-vesicle transition are mediated by the packing parameter of phospholipid–detergent systems

Temperature-induced micelle to vesicle transition: kinetic effects in the DMPC/NaC system

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