Effect of ionic strength and protein concentration on the transport of proteins through chitosan/polystyrene sulfonate multilayer membrane

Mathew, Jissy; Aravindakumar, Charuvila T.; Aravind, Usha K.

doi:10.1016/j.memsci.2008.08.026

Cited by 16 publications

(9 citation statements)

References 39 publications

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“…The solution pH is an important parameter for the ultrafiltration of proteins through charged membranes [2,17,22,23,31,32]. The electrical charge on both the protein and the membrane changes with pH due to acidic/basic groups on the protein and the membrane surfaces.…”

Section: Effect Of Phmentioning

confidence: 99%

“…In previous studies, ionic strength was found to be an important factor that may influence the transport behavior of proteins through the membranes [2,23,31,32]. Based on this fact, the ultrafiltration experiments were repeated with protein solutions containing 0.1 M NaCl.…”

Section: Effect Of Ionic Strength Of the Protein Solution On Protein mentioning

confidence: 99%

“…The layer-by-layer (LBL) assembly technique [20] was also used as an alternative method for creating either positively or negatively charged surfaces [21][22][23]. In this technique, a membrane with a charged surface is immersed in a solution of a macromolecule carrying opposite charges to those of the membrane surface.…”

Section: Introductionmentioning

confidence: 99%

“…Miller and Bruening (2005) prepared nanofiltration membranes with layer by layer deposition of hyaluronic acid-chitosan and reported the permeation of BSA and myoglobin through these membranes [21]. Aravind et al (2007) and Mathew et al (2008) modified commercial polyether sulfone microfiltration membrane with a uniform coating of chitosan/polystyrene sulfonate polyelectrolyte multilayer. They studied the transport of bovine serum albumin (BSA), ovalbumin and lysozyme through these membranes at their isoelectric points, as well as at pH levels below and above their isoelectric points.…”

Section: Introductionmentioning

confidence: 99%

“…They studied the transport of bovine serum albumin (BSA), ovalbumin and lysozyme through these membranes at their isoelectric points, as well as at pH levels below and above their isoelectric points. It was observed that the permeation characteristics of the proteins through the PEM-modified membranes depended on the number of deposited layers, as well as the pH, concentration and ionic strength of the protein solution [22][23].…”

Section: Introductionmentioning

confidence: 99%

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Preparation and characterization of polyacrylonitrile membranes modified with polyelectrolyte deposition for separating similar sized proteins

Mahlicli

Altınkaya

Yürekli

2012

Journal of Membrane Science

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a b s t r a c tOne of the challenges faced by ultrafiltration membranes is to separate proteins with a small difference in their molecular weights. Recently, some researchers tried to overcome this problem by using charged membranes. This study examined the use of layer by layer deposition of polyelectrolytes on the chemically-modified polyacyronitrile membrane to increase the selectivity of the ultrafiltration. The membranes were prepared by wet-phase inversion technique and polyethylenimine (PEI) and alginate (ALG) were chosen as cationic and anionic polyelectrolytes for the modification of the surfaces. Sieving coefficient data were obtained with myoglobin and lysozyme as model proteins. The influences of solution pH, ionic strengths of the protein and polyelectrolyte solution and the number of polyelectrolyte bilayers on both selectivity and throughput were investigated. The highest selectivity and throughput were achieved with the 1-bilayer PEI-ALG coated polyacrylonitrile (PAN) membrane. Increasing the number of coating bilayers or the ionic strength of the protein solution or adding salt into the polyelectrolyte coating solution decreased both the maximum selectivity and throughput of the modified membranes.

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