Effect of Ionic-Strength and pH Value in Mobile Phase on Enantio- Separation of BSA High Performance Liquid Chromatography Column

Effect of Chromatographic Conditions on Enantioseparation of Bovine Serum Albumin Chiral Stationary Phase in HPLC and Thermodynamic Studies

“…24,25 In the previous work, we reported enantioseparation of 4-phenyloxazolidine-2-thione, tryptophan, and pidotimod on a BSA-immobilized column and the effects of mobile phase pH and ionic strength was studied. 26 However, enantioseparation of amino acid derivatives is rarely reported to date by BSA-CSP on a silica support of full pore and small particle size. 21,22 Herein, enantioseparation of 12 chiral compounds such as N-(2, 4-dinitro phenyl) (DNP)-derived amino acids, liquiritigenin, ofloxacin, and 2, 8-diazabicyclo [4, 3, 0] nonane were carried out on a BSA-CSP column in reverse-phase mode.…”

“…In addition, BSA immobilized on substrates has a relatively dynamic structure and its conformation changes quickly in the range of mobile phase pH, ionic strength, organic modifiers, and temperatures without denaturing, further leading to a change of its recognition capability . In the previous work, we reported enantioseparation of 4‐phenyloxazolidine‐2‐thione, tryptophan, and pidotimod on a BSA‐immobilized column and the effects of mobile phase pH and ionic strength was studied . However, enantioseparation of amino acid derivatives is rarely reported to date by BSA‐CSP on a silica support of full pore and small particle size …”