Effect of intraovarian factors on porcine follicular cells: cumulus expansion, granulosa and cumulus cell progesterone production

Ježová, M; Scsuková, Soňa; Nagyová, Eva; Vránová, J; Procházka, Radek; Kolena, J

doi:10.1016/s0378-4320(00)00219-0

Cited by 26 publications

(10 citation statements)

References 38 publications

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“…Therefore progesterone has been suggested to be the trigger of AR in the cumulus layer in vivo. Unfortunately, this process still remains elusive since it is not clear how spermatozoa interact with cumulus cells and trigger the production/release of progesterone, although there have been reports that calcium mobilization is responsible for progesterone production (Jezová et al, 2001;O'Donnell et al, 2004).…”

Section: Involvement Of a Sperm-released Factor In Induction Of Ar Bymentioning

confidence: 99%

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

2011

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Fertilization is a process involving multiple steps that lead to the final fusion of one sperm and the oocyte to form the zygote. One of the steps, acrosome reaction (AR), is an exocytosis process, during which the outer acrosome membrane fuses with the inner sperm membrane, leading to the release of acrosome enzymes that facilitate sperm penetration of the egg investments. Though AR has been investigated for decades, the initial steps of AR in vivo, however, remain largely unknown. A well elucidated model holds the view that AR occurs on the surface of the zona pellucida (ZP), which is triggered by binding of sperm with one of the ZP glycosylated protein, ZP3. However, this model fails to explain the large number of 'falsely' acrosome-reacted sperms found within the cumulus layer in many species examined. With the emerging evidence of cross-talk between sperm and cumulus cells, the potential significance of AR in the cumulus oophorus, the outer layer of the egg, has been gradually revealed. Here we review the acrosome status within the cumulus layer, the cross-talk between sperm and cumulus cells with the involvement of a novel spermreleased factor, NYD-SP8, and re-evaluate the importance and physiological significance of the AR in the cumulus in fertilization.

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Section: Involvement Of a Sperm-released Factor In Induction Of Ar Bymentioning

confidence: 99%

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

2011

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“…In the pig, the receptor for EGF has been localised on the cumulus cells of both small and large antral follicles (Prochazka et al 2003). Treatment with EGF has been shown to induce expansion of porcine cumulus cells from small follicles (following treatment with FSH) and large follicles (Prochazka et al 2003), and stimulate progesterone production in porcine cumulus cells from large follicles, but not small follicles (Ježová et al 2001). Although treatment with db-cAMP has also been found to stimulate progesterone secretion by cultured porcine granulosa cells of small to medium-sized follicles (3-5 mm), this response was negated by the presence of EGF (Pescador et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Effect of follicle size and dibutyryl cAMP on the cAMP content and gap junctional communication of porcine prepubertal cumulus - oocyte complexes during IVM

Bagg

Nottle

Armstrong

et al. 2009

Reprod. Fertil. Dev.

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The lower ability of oocytes from prepubertal pigs to yield viable embryos than those from adult pigs appears, in part, a consequence of their reduced ability to accumulate cAMP during IVM. The present study examined the cAMP content of oocytes and cumulus-oocyte complexes (COCs), cumulus expansion and gap junctional communication (GJC) in COCs from 3- and 5-8-mm follicles during IVM. The effect of 1 mm dibutyryl cAMP (db-cAMP) treatment for the first 22 h of IVM was also examined for both follicle size classes. The cAMP concentration of oocytes from 5-8-mm follicles was threefold greater than that in oocytes from 3-mm follicles following 11 h of IVM (11.9 +/- 5.9 v. 3.6 +/- 1.8 fmol, respectively; P < 0.05). In the presence of db-cAMP, the cAMP content of oocytes from 3- and 5-8-mm follicles was no longer significantly different at 11 h IVM. The cAMP concentration of intact COCs from 5-8-mm follicles was significantly higher than that in COCs from 3-mm follicles at 11 h (1110.6 +/- 318.0 v. 116.9 +/- 55.7 fmol, respectively; P < 0.05). Despite maturation with db-cAMP, the cAMP content in COCs from 3- and 5-8-mm follicles at 11 h of IVM remained significantly different (15.1 +/- 4.9 v. 196.2 +/- 33.3 fmol, respectively; P < 0.05). The COCs from 3-mm follicles displayed lower cumulus expansion than did COCs from 5-8-mm follicles at both 11 h (cumulus expansion index (CEI) 1.0 +/- 0.1 v. 1.8 +/- 0.1, respectively; P < 0.01) and 22 h (CEI 1.9 +/- 0.3 v. 2.9 +/- 0.2, respectively; P < 0.05) of IVM. The level of cumulus cell-oocyte GJC decreased during IVM, with the number of GJC significantly greater in COCs from 3-mm compared with 5-8-mm follicles at both 6 h (613 +/- 55 v. 304 +/- 44 fluorescence intensity (FI), respectively; P < 0.05) and 11 h (644 +/- 99 v. 337 +/- 38 FI, respectively; P < 0.05) of IVM. By 22 h of IVM, the GJC of COCs from 3-mm follicles had decreased (227 +/- 18 FI) and was no longer significantly different to that of COCs from 5-8-mm follicles (139 +/- 15 FI; P > 0.05). Dibutyryl cAMP had no effect on the cAMP content, cumulus expansion or GJC of the whole COC. In conclusion, the results of the present study demonstrate that COCs from 3-mm follicles accumulate less intraoocyte and inter-COC cAMP, display lower cumulus expansion and maintain their cumulus cell-oocyte GJC for longer during IVM than do COCs from 5-8-mm follicles.

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“…Under in vitro conditions, porcine COCs from small antral follicles (2.0–3.0 mm diameter) secreted nearly twice as much P 4 than COCs from large follicles (5.0–8.0 mm diameter) (Ježová et al. ).…”

Section: Introductionmentioning

confidence: 99%

Effect of Follicle Size on In Vitro Maturation of Pre‐Pubertal Porcine Cumulus Oocyte Complexes

Töpfer

Ebeling

Weitzel

et al. 2016

Reprod Domestic Animals

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Very small follicles (<3.0 mm diameter) are over-represented on the surface of ovaries of non-cycling pigs, and the oocytes collected from these follicles generally have reduced developmental competence in vitro. This study examined the effect of follicle size on the nuclear maturation (n = 608), the potential of parthenogenetic activation (n = 243) and the cyclic AMP (cAMP) content of pre-pubertal porcine oocytes (n = 480). In addition, the influence of follicle size on steroid hormone synthesis was analysed. Cumulus oocyte complexes (COCs) flushed from small (2.5-4.0 mm) or large (4.5-6.0 mm) ovarian follicles were cultured for 0, 28 and 46 h. After 46 h of IVM, a greater proportion of oocytes from 4.5- to 6.0-mm follicles reach metaphase II (MII) compared with those from follicles with 2.5-4.0 mm of diameter (96.1 vs 77.0%, respectively; p < 0.001). Parthenogenetic activation of oocytes from large follicles produced higher developmental rates than oocytes from large follicles (p < 0.05). At 28 h, the IVM medium with oocytes from large follicles contained significantly more 17ß-oestradiol (E2 ) than the medium with oocytes from small follicles (5.55 vs 3.45 ng/ml, respectively; p < 0.05) and at 46 h, the medium with oocytes from small follicles contained significantly more progesterone (P4 ) than the medium with oocytes from large follicles (276.7 vs 108.2 ng/ml, respectively, p < 0.05). Porcine oocytes from large follicles have higher nuclear and cytoplasmic maturation capacities, but the differences did not appear to be cAMP-mediated. Our findings also suggest that COCs from small follicles undergo more intensive luteinization than COCs from large follicles. The results show that oocytes from follicles with a diameter greater than 4.0 mm are more suitable for in vitro studies.

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Effect of intraovarian factors on porcine follicular cells: cumulus expansion, granulosa and cumulus cell progesterone production

Cited by 26 publications

References 38 publications

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

Effect of follicle size and dibutyryl cAMP on the cAMP content and gap junctional communication of porcine prepubertal cumulus - oocyte complexes during IVM

Effect of Follicle Size on In Vitro Maturation of Pre‐Pubertal Porcine Cumulus Oocyte Complexes

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