Effect of Intramammary Injection of Nigella Sativa on Somatic Cell Count and Staphylococcus Aureus Count in Holstein Cows with S. aureus Subclinical Mastitis

Abstract

<b>Problem statement: </b>The seeds of <i>Nigella sativa</i> Linn. (Ranunculaceae) known as black&#13; seed or black cumin, are used in herbal medicine all over the world for the treatment and prevention of&#13; a number of diseases and conditions that include asthma, diarrhea and dyslipidaemia. <b>Approach:</b> In&#13; this study the effect of intramammary injection of <i>Nigella Sativa</i> Extract (NSE) in paraffin on quarter&#13; milk, quality and Somatic Cell Count (SCC) and the shedding pattern of <i>Staphylococcus aureus</i> were&#13; investigated. Thirty Holstein cows, naturally infected with <i>S. aureus </i>subclinical mastitis, were&#13; subjected to treatment with the NSE at a dose of 10 mL in paraffin (200 mg mL^&#8722;1) per day for 3 days,&#13; or with 10 mL paraffin as control. The injection areas were checked for adverse reactions. The daily&#13; milk production was measured before and after treatment. Intramammary injection of NSE caused a&#13; remarkable healing. Quarter milk samples were collected for bacteriological analysis and Somatic Cell&#13; Counts (SCC). The bacterial count moderately decreased in the treatment group. After the end of the&#13; treatment, the numbers of <i>S. aureus</i>-infected quarters and milk SCC tended to decrease in the NSEtreated&#13; cows. These clarifications were significantly higher one week post-treatment than pretreatment.&#13; Similar changes were not observed in the control group. <b>Results: </b>The results of the present&#13; study showed that the NSE has potential as a therapeutic agent for <i>S. aureus</i> infection causing&#13; subclinical mastitis of dairy cows and may contribute to the cow's recovery from mastitis. <b>Conclusion:</b>&#13; In conclusion, the results indicate that <i>Nigella sativa</i> might act as an antibacterial <i>in vivo</i> in dairy cows

​Effect of Dietary Inclusion of Cumin Seed (Cuminum cyminum) on Voluntary Feed Intake, Milk Yield, Milk Quality and Udder Health of Dairy Cows

“…Increase of SCC indicated inflammatory reaction and might be due to shift of leucocytes to the udder after entry of infection in the mammary gland and as a protective mechanism against infection (Murcus et al, 1994). Current results corroborated well with the findings of other workers (Sharma et al 2014;Singh et al 2019;Kumar et al (2011); Azadi et al (2011) and Preciado et al (2011). In Corroboration, Chandra et al (2017) also observed that the Somatic cell count (SCC) was significantly (P<0.05) lower in poly-herbal mixture and butyric acid supplemented groups as compared to control group in Murrah buffaloes.…”

Evaluation of in vitro antibacterial effect of essential oil and some herbal plant extract used against mastitis pathogens

“…S. aureus , which is known to be highly resistant to antibiotic treatment in mastitis, lives in the host's cells and becomes chronic by forming micro abscesses or granulomas in the mammary gland tissues (Azadi et al., 2011 ). In the bacterial isolation results, S. aureus bacteria were found to be 97.7% and E. coli 53.5% positive.…”

“…It has been observed that peppermint essential oil inhibits the growth of Gram‐negative microorganisms, especially Gram‐positive bacteria. It has been reported that the biological activity of the oil is due to its phenolic content (Azadi et al., 2011 ).…”

“…Gram-positive bacteria. It has been reported that the biological activity of the oil is due to its phenolic content (Azadi et al, 2011).…”

Evaluation of antimicrobial effect of Acacia nilotica plant extract and selected commercial disinfectants against some pathogens causing mastitis

“…The continuous and misuse of antibiotics in the treatment of mastitis pathogens and other diseases in both humans and animals has led to developing multidrug resistant organisms, so there is a great need for an alternative. The alternative must be cheap, sustainable and friendly to the environment [40]. Antimicrobials obtained from plants have much therapeutic potential and are effective in the treatment of infectious diseases in animals as well as humans.…”